STUDIES ON ASSOCIATION OF CAPSAICIN AND OLEORESIN CONTENTS ON INCIDENCE OF ANTHRACNOSE FRUIT ROT AND SEED QUALITY, AND VALIDATION OF DNA MARKERS LINKED TO PUNGENCY IN HOT PEPPER (Capsicum spp.)

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Date
2015-08-06
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Chilli (Capsicum annuum) in a highly valued spice cum cash crop. India is the largest producer of chilli in world. Anthracnose fruit rot caused by Colletotrichum Spp. is most serious disease reducing crop yield and quality. The present studies were carried out at the College of Agriculture, UAS, GKVK, Bengaluru. The results revealed that, days to fruiting ranged from (22-92 days), test weight (0.2972-9.74 g), germination (42-96 %), SVI-I (283-1489), seed protein (6.4-17.4 μg g-1), capsaicin (27-417 μg g-1), oleoresin 3- 23 %, ASTA colour value (2-16) and seed infection (0-82 %). The capsaicin content was negatively correlated with anthracnose disease and oleoresin was positively correlated with seed quality. Seed treatment with iprodine+ carbendizim @ 0.4 (%) eliminated anthracnose seed infection and improved seed quality. Storing dry chilli fruits in aluminium foils retained maximum seed germination (%) compared to vapour pervious containers. seed germination, test weight, TDH activity, seed protein, capsaicin and oleoresin decreased and seedling vigour and EC increased with storage period. Genotypes amplified single dominant band at 100 Kb with marker 7F/15R except AR-75, Byadagi Kaddi, Dyavanur Delux, G4, Gouribidanur Local, HMT-1 for pungency. A low pungent parent Paprika from C. annuum was hybridized to highly pungent parent C. chinense Assam Bhut Jolokia to produce inter specific hybrid and the six generation mean analysis revealed that, higher seedling length and the higher seedling emergence were predominantly under the control of genes with dominance effect and the dominance effect were unidirectional as evidenced by higher magnitudes of dominance gene effects.
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