MICROPROPAGATION STUDIES IN SELECTED GENOTYPES OF TOMATO (LYCOPERSICON ESCULENTUM MILL.)
Loading...
Files
Date
2001
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu
Abstract
The present investigation was carried out at Centre for Biotechnology, FOA, Campus Udeywalla, SKUAST-J with objectives to :- 1) refine micropropagation protocol in selected genotypes / hybrids; (2) assess feasibility of alternative solidifying agents: and (3) standardise hardening requirements.
The seeds of tomato hybrids TH802 and TH2312 and their parents after treatment with 70% C2H5OH followed by 3 mindip in 4% NaoCL were put on germination on MS medium. For studies on refinement of micropropagation protocol the explants (shoot tip, cotyledon and hypocotyl) taken from 14 days old seedlings (grown on 1/2 MS basal medium), were cultured on MS medium supplemented with different concentrations of BAP (2.22, 4.40, 6.62, 8.90, 11.10 μM). It was observed that cotyledon explants gave best response (100%) in four genotypes namely TH802, Haelani, VFN8 and Punjab Chuhara. The other genotypes Accession-2 and TH2312 showed 80% and 83.3% response, respectively. In all the genotypes shoot primodia emerged after four weeks of subculturing. These shoot primordia enlarged into microshoots. The microshoots thus obtained were subcultured on MS medium supplemented with different concentrations of BAP (2.22, 4.40, 6.62, 8.90, 11.10 μM) for multiple shoot formation studies. Genotypic differences were observed for shoot multiplication. The maximum number of shoots/ explant were observed in TH802 (5.17), a pattern similar to better parent (Accession-2) (5.05). Likewise hybrid TH2312 gave response similar to one of its parent VFN8. Feasibility of different gelling agents i.e. Agar, Isubgol and Sago was also tested. The MS medium with Isubgol gelling agent gave highest number of shoots/ explant in all the genotypes i.e. TH 802 (5.61) and TH 2312 (5.40) as well as their parents i.e. Haelani (4.95) and Accession-2 (5.44) and VFN8 (5.33) and Punjab Chuhara (5.06) respectively.
For rooting studies plantlets of hybrid TH802 were put on MS medium supplemented with different concentrations of IBA (0.00, 2.46, 4.90, 7.36, 9.80 μM). Genotypic differences were also observed for rooting. The maximum average number of roots was (17.55) with maximum average root length (8.62)cm after 2 weeks interval were observed in TH802. The plantlets were hardened by putting under high light intensity for 2-3 hrs. followed by treatment with 0.5% glycerol for 1 m. And then the plantlets were kept in green house for 3 weeks and subsequently transferred to the field.
Description
Keywords
Citation
No. of references 72