ISOLATION AND CHARACTERIZATION OF HEAVY METAL RESISTANT BACTERIA FROM INDUSTRIAL AND MINING AFFECTED AREA OF CHHATTISGARH

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Date
2017
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Indira Gandhi Krishi Vishwavidhyalaya, Raipur
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The present investigation was carried out to assess the effect of heavy metal (HM) resistant bacteria from industrial and mining affected area of Chhattisgarh. Sixty-five HM resistant bacteria, isolated from soil and water, were screened for their ability to use arsenic (As), nickel (Ni) and cadmium (Cd). Out of 18 selected isolates, 9 As, 5Ni and 4 Cd resistant bacteria were characterized morphologically and biochemically. Most of the isolates were gram negative except three isolates i.e., MCdC3, ICdC2 and MCdC6. Out of nine isolates, RSN4 was able to tolerate upto 700mM of sodium arsenate. In case of isolate INi4 and MCdC3 can survive in 10 mM concentration of nickel chloride and cadmium chloride respectively. Therefore, three isolates i.e., RSN4, INi4 and MCdC3 were finally selected for bioremediation assay of As, Ni and Cd. In all three cases, growth of bacteria was affected in presence of HM. Growth of RSN4 in absence of sodium arsenate increased rapidly up to 0.854 OD at 600nm on 2nd day of inoculation but decline after whereas in case of RSN4 with sod. Arsenate, growth was slow but extended upto 8th day of inoculation. In case of Ni and Cd bacterial growth increases rapidly in presence of HM. Surprisingly, extended stationary phase of INi4 was observed in presence of nickel chloride. Sequencing of 16S rRNA gene of RSN4 revealed that sequence has similarity (92%) with genus Klebsiella sp.
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