Development and evaluation of Cu- chitosan nanocomposite for its antifungal activity against post flowering stalk rot (PFSR) disease of maize caused by Fusarium verticillioids (Sheldon)”
Loading...
Date
2017
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
MPUAT, Udaipur
Abstract
The purpose of this study was to synthesize Cu-chitosan NCPs and evaluates
its antifungal activities against Fusarium verticillioids causing post flowering stalk
rot (PFSR) disease of maize. Cu-chitosan NCPs were prepared based on the ionic
gelation of chitosan with tripolyphosphate anions and Copper ions. The
physicochemical properties i.e. particle size (295.4 nm), polydispersity index (0.28),
zeta potential (+ 19.6 mV), functional group analysis (chitosan, TPP and Cu
interaction by frequency distribution), shape (sphere), morphology (highly porous),
elemental composition (C, O, P, N and Cu) were determined by DLS, FTIR, TEM,
SEM and SEM-EDS. Further Cu- encapsulation was analyzed by AAS under
different pH, agitation period and rate. Among the treatments, 0.1% Cu-chitosan
NCPs found effective in inhibition of mycelial growth and spore germination of F.
verticillioides and which was further explained by SEM studies. In Surya local
variety, seed vigour index was found higher in almost in all Cu-chitosan NCPs
compare to control in both 4 and 8 hrs of seed treatments in Petri-plate experiments.
In Pratap hybrid-1 seed vigour index was found higher in 4 hrs treatments. Cu
content (mg/gm) in all the treatments increased as the concentration of Cu- chitosan
NCPs increased in both varieties in 4 and 8 hrs seed treatments analyzed by AAS.
Chlorophyll a and b was found higher in Surya local (4 hrs seed treatment) in 0.02%
and 0.14% Cu-chitosan NCPs as compared to Pratap hybrid-1. The enhanced
activity of defense enzymes were found in the order of peroxidase (POX) >
phenylalanine ammonia lyase (PAL) > polyphenoloxidase (PPO) in this study.
Overall the effect of NCPs mainly in 4 hrs seed treatment in Surya local was
effective in enhancing the defense enzymes as compared to 8 hrs treatments. In
Pratap hybrid-1 not much rational effect was noticed in NCPs treatments. Cuchitosan
NCPs at the concentration of 0.1% and 0.14% found effective in controlling
PFSR in pot experiments. In Surya local (4 hrs treated seeds) PEDC was 48.1% at
0.1% Cu-chitosan NCP. In Surya local (8 hrs treated seeds) 49.6% PEDC was
observed at 0.1 and 0.14% Cu-chitosan NCPs. In field experiments, Cu-chitosan
NCP at the concentration of 0.06% found effective in controlling PFSR disease.
Significant disease control of PFSR was observed (33.9%) at 0.06% Cu-chitosan
NCPs in Surya local (4 hrs treated seeds). All together Cu-chitosan NCP has
remarkable potential as antifungal agent against PFSR of maize in pot condition as
well as in field condition.
Description
Development and evaluation of Cu- chitosan nanocomposite for its antifungal activity against post flowering stalk rot (PFSR) disease of maize caused by Fusarium verticillioids (Sheldon)
Keywords
null
Citation
Choudhary and Saharan, 2017