ANALYSIS OF MORPHO-MOLECULAR DIVERSITY, COMBINING ABILITY AND STABILITY IN MAIZE (Zea mays L.)

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Date
2013-11-20
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University of Agricultural Sciences GKVK, Bangalore
Abstract
An investigation was carried out at ZARS, V.C. Farm, Mandya; ARS, Bavikere and Farmer's field, Yatnahalli village, Ranibennur Tq. to analyze the morphological and molecular (SSR) diversity, combining ability of inbreds and stability of hybrids for 16 morphological characters during kharif 2012. Pooled analysis of variance revealed significant differences among 100 inbreds and 225 hybrids for all the 16 characters. One hundred inbreds were grouped into 10 clusters and cluster I (48) accommodated maximum number of inbreds. The character days to brown husk contributed highest for total divergence and the inbred MAI- 706 recorded highest mean values for more number of characters. At the molecular level, inbreds were grouped into seven clusters and cluster III was the largest with 35 entries. A total of 213 SSR alleles were detected and the marker umc2078 sampled highest number of alleles and maximum PIC was observed for umcl542. There was no agreement between morphological and molecular diversity. Predominance of additive genetic variance was noticed for characters grain yield, test weight, grain rows per cob, cob length and cob girth. The inbreds LM-13,V-1712-1, V-1154 and CIMMYT-5 were good general combiners and the crosses MAI-117 x LM 13, Z-62-67 x MAI 105 and Z-62-55 x LM 13 were good specific combiners.The crossesCIMMYT-5 x LM 13, LTP-1 x SKV 50 and CIMMYT-5 x SKV 50 manifested significant standard heterosis for highest number of characters.The test hybrids V-2232 x MAI 105, V-1649 x MAI 105, CIMMYT-5 x LM 13, V-1712-1 x MAI 105, Z-63-30 x LM 13, V-632-112 x LM 13, V-1168 x LM 13, V-291-2 x LM 13, V-632-67 x LM 13, CML-172 x MAI 105, V-1154 x LM 13, V-241-2 x LM 13, CIMMYT-47 x LM 13, Z-51-1 x LM 13, HKI-193-1 x LM 13, V-2232 x LM 13 and V-1649 x LM 13 were stable with high grain yield and could be further evaluated for confirmation of their superiority.
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