CGMS BASED MALE STERILE LINES: MOLECULAR CHARACTERIZATION, STABILITY AND UTILIZATION FOR HYBRID DEVELOPMENT IN PIGEONPEA [Cajanus cajan (L.)Millsp.]
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Date
2014-06-30
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Abstract
The present investigation was carried out using 17 male sterile and their maintainer lines, 22 restorer lines and 141 CGMS based pigeonpea hybrids along with check varieties to derive information on phenotypic and molecular characterization of A, B and R lines, stability of male sterility, yield and yield contributing traits over the locations, estimation of heterosis and combining ability in hybrids, screening of genotypes for Fusarium wilt and Sterility Mosaic Disease (SMD) in hybrids and parents and identification of new restorers.
Genotypes exhibited enormous phenotypic variability, characterisation successfully differentiated anthocynin colour on hypocotyls present in 50 but absent in five genotypes such as ICPA-2043, ICPB-2043, GT-307A, GT-307B & RAJA.
The CMS lines ICPA-2092 and GT-308A were highly stable and recorded mean pollen sterility of 100% across the locations and seasons, these can be utilized in hybrid breeding programme. Stability parameters for seed yield/plant indicated that ICPA-2048-4 had stable performance and well adapted to all the environments.
The parents ICPA-2078, GT-288A, GT-308A, WRP-1A, GPHR-8-11, AKT-9913 and JKM-197 were good general combiners for seed yield per plant. When these parents were used for crossing, their cross combination likely to give desirable transgressive segregants in advance generations and may be utilized for selecting better and high yielding genotypes.
Female parent ICPA-2047 had resistance for both SMD and Fusarium wilt. Male parents ICP-7035 and RAJA had resistant reaction for SMD, while GRG-811 showed resistant and moderately resistant for wilt and SMD respectively, hence; these can be used as donor parents for resistant hybrid development. Two hybrids ICPA-2047 x GRG-811 (1683 kg/ha) and ICPA-2047 x BDN-2 (2288 kg/ha) were resistant for both the diseases with percent disease incidence value of 8.06 & 9.38 for fusarium wilt and 5.88 & 6.25 for SMD respectively.
Male parents PKV-TARA,VIPULA, BSMR-853, GPHR-8-11, ICP-7035, RVKP-260, GRG-811, ASHA, JKM-197 and GC-11-39 identified as good restorers since they restored fertility on male sterile lines and exhibits 100 percent fertility in hybrids.
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