IMPROVING BIOMASS AND YIELD POTENTIAL IN Ganoderma lucidum STRAINS FOR THE PRODUCTION OF TERPENOIDS
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Date
2011
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PAU Ludhiana
Abstract
Three strains of Ganoderma lucidum (GL-1, GL-2 and GL-3) were used in present studies for biomass and fruit body production and extraction of triterpenes. Mycelial biomass was raised in Mushroom Complete Medium according to central composite rotatable design using design expert statistical software. The conditions were optimized and were found to be 29.50C to 34.30C temperature, 6.1 to 7.0 pH, 30.5 to 32 days of incubation period and 50.9 to 52 rpm agitation for getting maximum mycelial biomass. Fruit bodies were raised on wheat straw supplemented with wheat bran and rice bran. Maximum biological efficiency of 16 per cent was obtained in GL-1 at 5 per cent level of supplementation of wheat bran. Triterpenes were extracted from biomass as well as powdered fruit bodies and separated in hexane, dichloromethane and ethylacetate in order of increasing polarity. Fruit bodies contained maximum amount of triterpenes and yielded about 7.8 to 7.9 per cent from GL-1 and GL-2 respectively. The colours of the obtained masses were ranged from yellow to dark brown. Thin layer chromatography (TLC) and mass spectroscopy (MS) was carried out for further resolution of extracted triterpenes in different solvents using benzene: ethylacetate (5: 1 v/v) and chloroform: benzene (7:1 v/v) by TLC. There is resolution of a no. of compounds in TLC. Their Rf values ranged from 0.11 to 0.96 in two different solvent systems. Lower Rf values represented bond forms of triterpenoid like compounds and higher Rf values showed the presence of released forms of triterpenes. MS of the samples reveals the presence of Ganoderic acid A, AM- 1, B, J, H, Lucidenic acid N & A, Ganolucidic acid A & D and two new triterpenes 3β- hydroxyl-4,4,14- trimethyl- 7,11,15- trioxochol- 8-en-24-oic acid and 7,15-dihydroxy- 4,4,14- trimethyl-3,11-dioxochol-8-en-24-oic acid.
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