Propagation of Chlorophytum borivilianum Sant . et Fernand in vitro for conservation
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Date
2014
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Birsa Agricultural University, Kanke, Ranchi, Jharkhand
Abstract
An efficient protocol for rapid in vitro propagation of valuable medicinal plant
Chlorophytum borivilianum by using shoot tip as explants were done. This plant is used
in the traditional medicinal system for enhancing male potency, curative for physical
weakness, natal and post natal problems, remedy for diabetes and arthritis. It is an
aphrodisiacs agent used for preparation of general sex tonic, and immunity-improving
drug. The in vitro plantlets production system was investigated on Murashige and Skoog
medium with different concentrations and combinations of different hormones such as
BAP alone and in combination with AdSO4, with and without IAA and KIN for shoot
multiplication. Shoot multiplication was induced on shoot tip. Multiplication was best
(88.83%) in shoot tip explants when grown on MS medium supplemented with BAP (6.0
mg/l). Rooting on excised shootlets was highest (91.31%) in MS medium supplemented
with 2.0 mg/l IBA along with 2.0 mg/l IAA. Rooted plantlets were transferred to the
portray for hardening. The potting mix used was sterile coco peat. After plantation the
portrays were kept under poly tunnel to retain almost 100% humidity. After 15 days, the
new leaves and roots came out and the plantlets were transferred to poly bags containing
soil: sand: FYM in 1:1:1 proportion and kept under the shade in green house for
secondary hardening. The survival percentage in green house was found 95.83% .
Transferred safed musli plantlets established better under net house condition compare to
direct exposure into the field. Micro propagation seems to be the best method compared
with other methods of multiplication. In Safed musli in vitro method increased the rate of
multiplication while combating the problem of long tuber dormancy to facilitate for
growing more than one crop per year.
Description
Propagation of Chlorophytum borivilianum Sant . et Fernand in vitro for conservation
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