Genetic transformation of Banana (Musa sp.) through Agrobacterium tumefaciens
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Date
2013
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Birsa Agricultural University, Kanke, Ranchi, Jharkhand
Abstract
Banana and plantains (Musa spp.) are considered the fourth important staple food crop
in the world after rice, maize and wheat. The crop is the most nutritious of all food
crops and provides food and income to millions of people around the world.
Available techniques for the genetic transformation with important genes could
overcome some of the agronomic and environmental problems limiting conventional
breeding of bananas. Although it is possible to transform bananas, broad application of
the technology is limited because of the low overall efficiency and lack of reliability
of the technique. Despite playing a key role in the economy of many developing
countries, its production and as such export is constrained by many biotic as well as
abiotic factors.
This research reports on the potential of Agrobacterium for the transformation of
Banana. The meristem of the suckers was used as the explants for the aseptic culture
establishment of banana. The banana culture was established using MS medium
supplemented with 6.0 mg/l BAP, 0.2 mg/l IAA and 100.0 mg/l AdSO4 which was
used for the infection with Agrobacterium tumefaciens. The npt II gene was used as
reporter, the genes is under the regulation of CaMV35S promoter, for quantification of
transformants in the binary vector, pCAMBIA 2300. Results obtained indicated that
the 45 min. co-culture was found most suitable for achieving transformants in the
media supplemented with 200.0 mg/l kanamycin. The Kanamycin tolerance test was
performed to check the sensitivity of explants, which was observed best at 200.0 mg/l
and was used for the selection of the transformants. Only about 22.22% explants
showed resistance on kanamycin stressed media upon 45th day at 45 min. co-culture
with bacteria.
Description
Genetic transformation of Banana (Musa sp.) through Agrobacterium tumefaciens
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