ASSESSMENT OF GENETIC DIVERSITY AND DIFFERENTIAL REACTION OF LENS SPECIES TO PULSE BEETLE, CALLOSOBRUCHUS CHINENSIS (L.
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Date
2014
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Division of Plant Genetic Resources Indian Agricultural Research Institute New Delhi
Abstract
Lentil (
Lens culinaris
L.), an important
food
legume,
cultivated in
many developing countries.
Indigenous lentil being ‘microsperma’ and ‘pilosae’ type has narrow genetic base and its
broadening is essential for the varietal improvement to increase lentil yield. Different species in
gene pool contains a vast diversity which needs to be assessed and
use in crop improvement
programmes. Also, its full yield potential is not realized due to various constraints including
insect
-
pests. Of these, pulse beetle (
C.
chinensis
) is important having very wide host range and
different strains causes, both quantit
ative and qualitative losses during storage. Adverse effects
of pesticides, there is need for ecofriendly approaches of pest control including host plant
resistance. Hence, different
Lens
spp. were analyzsed for genetic diversity assessment and
evaluated f
or differential reaction of
C. chinensis
to identify the sources of resistance
.
The
genetic diversity analysis was assessed in 50 accessions of different
Lens
species using ISSRs
markers. The collection included accessions of cultivated species
L
. culinari
s
and its six wild
species, viz.,
L.
culinaris
subsp.
odemensis,
L.
culinaris
subsp.
orientalis,
L. orientalis,
L.
nigricans,
L. lamottei
and
L. ervoides.
The selected 23 ISSR primers amplified a total of 368
bands with an average of 16 bands per primer. The maximum number of ISSR bands was
amplified by the primers ISSR
-
34
and ISSR
-
835. The primers ISSR
-
807, ISSR
-
809, ISSR
-
827,
ISSR
-
847, ISSR
-
28 and ISSR
-
37
were found to be very useful for diversity assessment of the
genus
Lens
. UPGMA cluster analysis revealed clear separation of all the species with two main
clusters.
Principal Coordinate analysis of the 368 markers using 23 ISSR primers revealed that
only
11.03% of the total variation was explained by first three Principal Coordinates.
Accessions were also evaluated for their reaction to pulse beetles under no
-
choice artificial
infestation conditions. Both inter and intraspecific variations among the accessions were
observed in terms of various parameters
viz.,
oviposition, development p
eriod, adult emergence,
number of emergence holes, weight loss and growth index (GI). The GI ranged from 0.00 to
3.89, based on GI,
accessions were categorised as highly resistant (15 accessions, GI = 0.00),
resistant (14, GI=0.00 to 1.00), moderately resi
stant (16, GI=1.00 to 2.00) moderately
susceptible (3, GI
=
2.00 to 3.00) susceptible (3, GI
=
>3.00) to attack of
C. chinensis
.
Within a species, the accessions were in different categories viz.,
L. ervoides
as highly resistant
and
resistant;
L.
lamottei
ac
cessions as highly resistant, resistant and moderately resistant;
L.
nigricans
and
L.
orientalis
as resistant and moderately resistant;
L. culinaris
subsp
orientalis
and
L. culinaris
subsp
odemensis
as resistant, moderately resistant and moderately susce
ptible
and
L
.
culinaris
as susceptible. Thus, based on the parameter of GI the species have been
arranged in the order of their resistance to
C. chinensis. L. ervoides
>
L. lamottei
> L. nigricans
= L. orientalis
> L.
culinaris
subsp.
orientalis
= L.
culinaris
subsp.
odemensis
> L. culnaris
i.e.
L. ervoides
was highly resistant and
L. culnaris
was the most susceptible.
While, comparing
molecular with resistant/ highly resistant infestation behavior no clear and unambiguous
clustering was observed in th
e dendrogra
Description
t-9039
Keywords
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