EVALUATION OF OXIDATIVE STRESS IN TRANSITION COWS AND POST PARTUM ENDOMETRIAL CYTOLOGY
Loading...
Date
2013-03
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
SRI VENKATESWARA VETERINARY UNIVERSITY , TIRUPATI – 517502. (A.P.) INDIA
Abstract
ABSTRACT :
The transition period is physiologically the most important, highly sensitive and venerable in the reproductive life of dairy cows. The physiological and metabolic changes occurring in peripartum period imposes oxidative stress and directs the subsequent reproductive events in dairy cows. The objective of the study was to evaluate oxidative stress, antioxidant status and endometrial cytology for predicting the uterine involution in cows. A total of 14 multiparous crossbred cows maintained in a commercial farm were assigned to group I (8 Healthy) & II (6 Infected). Blood samples were collected periodically on day -10, 0, 10, 25 and CUI. Spectrophoto-metric procedures were adopted to determine concentrations of MDA, Vit.E, GSH, glucose, total proteins and SOD and Catalase activity, in addition to endometrial cytological smears on day 25 and CUI. Neutrophils counts were decreasing after day 25 and in infected group II the counts were significantly higher. Values of MDA, Vit.E, GSH and Catalase activity were significantly different (p≤0.05) between collections in group I and II while, glucose and total proteins remained within physiological ranges. Progesterone concentration significantly different (p≤0.05) between collections in group I and II. The cows in group II had high lipid peroxidese and low antioxidant status during early part of the experiment and was affected with uterine infections.
It was concluded that there were significant differences between two groups of cows investigated. The high MDA profiles recorded on day 0 and low antioxidant status observed in group II animals might have compromised host`s immune defense and suffered them from uterine infections. Further, the presence of neutrophils significantly in high percentages in uterine cytological smears obtained at CUI (after morphological completion of uterine involution) indicates the persistence of infection after declaring completion of involution. From the present study, it was suggested that the routine rectal examination of the tract is insufficient to conclude completion of involution. It was felt that in routine practice the endometrial cytology may be adopted in clinical diagnosis of uterine involution.
Description
THESES
Keywords
null