CHARACTERIZATION OF KINNOW MANDARIN CLONES AND MUTANTS
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Date
2014
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Division of Fruits and Horticultural Technology Indian Agricultural Research Institute New Delhi
Abstract
Present investigation was carried out to characterize Kinnow mandarin clones,
induced mutants and parental genotype at the Division of Fruits and Horticultural
Technology, I.A.R.I., New Delhi during 2013-14. Four clonal selections, plants
developed from bud woods treated with different concentration of EMS (0.05%, 0.1%,
0.2% and 0.5%) and gamma irradiation (5, 10, 15 and 20 Gray) along with parent
Kinnow (control) were used for studying the morphological, physiological, biochemical
and molecular variations.
Stimulated growth was recorded at lower doses of mutagenic treatment. i.e., 5
Gray (42.05%) and 0.05% EMS (31.43%) over control while inhibition in the growth
parameters were recorded at 0.5% EMS and 20 Gray. Among clonal selections maximum
increase in plant height was recorded in 12EEA 1/4 (20.57%). Significant reduction in
leaf area, leaf fresh mass, leaf dry matter content, number of stomata and total
chlorophyll content was observed in 0.5% EMS and 20 Gray treatments. Reduction was
also observed in the above treatment with respect to leaf gas exchange parameters such as
photosynthetic rate (A), stomatal conductance (gs) and intrinsic water use efficiency
(WUEi). Membrane Injury Index (MII) was significantly reduced at 20 and 15 Gray
(0.11, 0.21). Two fold increase and 0.95 fold decrease in proline content was recorded at
0.2% EMS and 20 Gray. Phenol content was detected maximum at 20 (19.47 mg g-1
FW)
and 15 Gray (18.37 mg g-1
FW). Irradiation and EMS-induced mutagenesis had
significant impact on the catalase and peroxidase activity of Kinnow leaves recording
1.85 fold increase in catalase activity at 20 Gray and 1.36 fold increase at 0.5% EMS
over control. Irradiation treatment up regulated superoxide dismutase (SOD) activity by
23 and 19.66 fold at 20 and 15 Gray respectively.
Genetic diversity among Kinnow clones and mutants including the parent was
also studied using SSR markers. Out of 87 primers tested, 11 primers showed
polymorphism and amplified 14 alleles. These polymorphic primer pairs could amplify
1–2 alleles /primer pair giving average 1.27 amplicons/primer pair. The PIC value ranged
from 0.14 to 0.99 and indicated that markers used were quite informative. Dendrogram
based on Jaccard’s similarity coefficients showed that genetic similarity between lines
ranged from 0 to 0.9. Genotypes were grouped into two clusters based on genetic
distances and the UPGMA grouping could clearly discriminate the genotypes effectively.
All the mutants and clones distinctly grouped different from the parent indicating that
there were distinct molecular changes in these mutants and clones.
Description
t-8999
Keywords
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