Assessment of Variability and Standardization of Protocol for Micropropagation in Spine gourd (Momordica dioica Roxb.)
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Date
2016
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MPUAT, Udaipur
Abstract
The present investigation entitled “Assessment of variability and standardization of protocol for micropropagation in spine gourd (Momordica dioica)” was carried out in two phases: in-situ variability study and to standardize the protocol for micropropagation in Momordica dioica. For in-situ study the variability was studied among thirty four randomly identified wild growing genotypes around Udaipur (Rajasthan). To standardize the micropropagation protocol, regeneration of plantlets from leaf and nodal explants were accomplished successfully using MS basal media supplemented with different growth regulators.
Analysis of variance for in-situ study revealed that mean squares due to genotypes were significant for all the traits except spine length in both the years, fruit specific gravity in the first year and chlorophyll content in second year thereby, showing considerable amount of variability for most of the characters and were amendable to improvement.
In terms of per se performance, genotype MPK-009 was superior for most of the characters followed by genotype MPK-006. These genotypes were identified as desirable ones in variability studies.
Magnitude of GCV and PCV was high for protein content, fruit weight, fruit volume, ascorbic acid and TSS. High heritability was recorded for TSS only. The high genetic gain was depicted by fruit weight, fruit volume, protein content and ascorbic acid.
When yield data are not available, the character like fruit weight may be considered as target character. In the present investigation it was positively and highly significant correlation with fruit length, fruit diameter, fruit volume and number of seeds per fruit at both levels in first year whereas fruit specific gravity and ascorbic acid at both levels in second year.
The regression analysis revealed that in in-situ observations of all the 8 characters contributed towards fruit weight was significant i.e., 91.8 and 78.7 per cent variability respectively in both the years. The regression analysis revealed that contribution of all the 8 characters towards ascorbic acid was significant in second year only i.e., 68.0 per cent variability.
The establishment of an efficient plant regeneration protocol in spine gourd using two types of explatns viz., in vivo nodal and leaf as well as in vitro nodal and leaf explants, respectively, has been used in the present experiment. Among the different treatments of TDZ and BA, maximum shoot induction was obtained from 1.5 mg/L TDZ containing media which showed a maximum shoot induction of 71.6 per cent. In the second set of media, BA alone and in combinations with Kin and NAA were evaluated. Among all the treatments, the highest shoot induction response of 83.3 per cent was noticed in 1.5 mg/L BA supplemented media. Shoot regeneration rate of 100 per cent was obtained from the in vitro nodal segments in TDZ (0.5-2.5 mg/L) alone, BA (1.0-2.0 mg/L) alone, BA in combination with Kin (2.5+0.5 mg/L) and BA (2.5-3.0mg/L)+0.5 mg/L NAA fortified media. Among the various cold treated in vitro leaves, the leaves which were treated for 24 hr showed maximum bud breakage and highest shoot regeneration response (46.6 per cent) on media containing 3.0 mg/L TDZ. Comparison among all multiplication media, revealed that, 0.5 mg/L BA and in combinations with 1.0 mg/L Kin was found to be most suitable for shoot multiplication i.e., 8.5 number of shoots per explant. Out of the different hardening media tested, cocopeat in combination with vermiculite achieved highest survival rate of plants i.e., 73.3 per cent which was significantly different from others.
Description
Assessment of Variability and Standardization of Protocol for Micropropagation in Spine gourd (Momordica dioica Roxb.)
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Citation
Jamatia and Kaushik, 2016