INVESTIGATION ON STONY PIT DISEASE OF PEAR
Loading...
Date
2014
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
UHF,NAUNI
Abstract
ABSTRACT
Surveys conducted during 2013 in pear orchards of Kullu, Mandi and Shimla districts of
Himachal Pradesh revealed the incidence of stony pit disease ranging from 10 to 65 per cent.
Prominent symptoms associated with different cultivars were leaf vein yellowing, chlorotic leaf spots,
fruit deformities with formation of pits, corky tissue beneath the pits and misshapen fruits alongwith
russeting on the surface of fruits. Serological detection through DAC and DAS-ELISA resulted in the
detection of apple stem pitting virus (ASPV) and apple chlorotic leaf spot virus (ACLSV) either alone
or in combination in all samples of eight cultivarsmarked during survey. Biological detection of one
of the isolate (Red Bartlett) containing infection of ASPV and ACLSV on herbaceous hosts resulted
in the production of symptoms on Chenopodium amaranticolor, C. quinoa, Nicotiana glutinosa, N.
tabacum and a few other hosts. Biological detection on woody indicators (Quince A, Quince B-29 and
Quince C) resulted in the development of typical viral symptoms in all the indicators by double
grafting method. Leaf and petal samples drawn during April-May and fruits during August were
found suitable for the detection of ASPV and ACLSV in DAS-ELISA. Association of ASPV with
viral symptoms in pear was also confirmed by RT-PCR. DAS-ELISA based serological screening
resulted in the selection of 16 plants of seven cultivars free from ASPV infection.
Description
Keywords
null