Adaptation of Classical Swine Fever Virus in Cell Culture System and Confirmation by Electron Microscopy
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Date
2017-03
Journal Title
Journal ISSN
Volume Title
Publisher
Indian Veterinary Journal
Abstract
Adaptation studies of field isolates of Classical
Swine Fever (CSF) virus was carried out in
Porcine Kidney (PK 15) cell line. Bulk culture of
the virus at 25th passage was subjected to purifi -
cation and concentration by ultracentrifugation
and also con rmation by RT-PCR using NS5B
primers. The Transmission Electron Microscopy
(TEM) studies of CSF virus revealed 40-50 nm
virus like particles which were surrounded by
an asymmetrically arranged poorly defined sac
like membrane. This was taken as confirmation
for the adaptation of the CSF virus in PK 15 cell
line.
Description
Keywords
Veterinary Science, Animal Biotechnology