BIOCHEMICAL AND MOLECULAR ASPECTS GOVERNING DROUGHT TOLERANCE IN RABI SORGHUM IN COMPARISON WITH EXISTING STAY-GREEN GENOTYPES
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Date
2013
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Mahatma Phule Krishi Vidyapeeth, Rahuri.
Abstract
The present investigations was undertaken to evaluate the levels of osmolytes, activities of antioxidative enzymes, proline metabolizing enzyme, P5CS and nitrate reductase under PEG-6000 –induced osmotic stress and water stress created by withholding irrigation at pre- and post-flowering growth stages in sorghum genotypes. The isozymes of peroxidase and superoxide dismutase and soluble protein profile were resolved on native and SDS-PAGE. The molecular diversity among the selected drought susceptible, tolerant and stay-green sorghum genotypes using RAPD were also carried out. A total of forty eight rabi sorghum genotypes were evaluated for proline and glycine betaine accumulation under PEG6000 –induced osmotic stress (-0.5 Mpa). The genotypes, RSLG-262 (Maulee) and RSV-1366 accumulated the highest proline content of 0.956 and 1.263 µmoles g-1 FW, respectively. The leaves of genotypes, RSV-458 (Anuradha) and Hadgaon local accumulated highest glycine betaine content of 33.48 and 37.14 µmoles g-1 FW, respectively, whereas the susceptible genotypes, CSV-18 and RSV-1045 recorded
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Agricultural Biochemistry