"EFFECT OF DIFFERENT SEED BULKS ON GENETIC POTENTIAL OF PARENTAL LINES OF COTTON HYBRIDS"

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Date
2004-06-30
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MAHATMA PHULE KRISHI VIDYAPEETH, RAHURI-413 722
Abstract
With a view to study the effect of methods of bulks of seeds of selected plants on maintaining genetic purity, present investigation "Effect of different seed bulks on genetic potential of parental lines of cotton hybrids" was carried out at Cotton Improvement Project and Seed Technology Research Unit, M.P.K.V., For this investigation four parental lines of cotton viz., RHC-003, RHC-004, RHC-006 and RHCb-001 and their bulks viz., superior, model and ordinary were sown during kharif 2003. The trial was conducted in factorial randomized block design with three replications. The morphological characters of seeds, seedlings, plant, flower, boll and fibre were studied for varietal identification. Some parental lines showed common characters and could be differentiated by other characters. For identification of parental lines these different morphological characters were used. The observations were recorded for important characters viz., initial plant count/plot, final plant count/plot, days to 50 % flowering, days to maturity, plant height (cm), number of monopodia per plant, number of sympodia per plant, number of bolls per plant, boll weight (g), seed cotton yield/ plant, seed cotton yield (kg/ha), lint yield (kg/ha), seed yield (kg/ha), ginning percentage, 2.5 % span length (mm), micronarie value (ug/inch), fibre strength (g/t) and uniformity ratio (%). All the four parental lines viz., RHC-003, RHC-004, RHC-006 and RHCb-001 differed significantly for above characters. All the three bulks did not differ significantly due to above characters except 2.5 % span length (mm) and interaction effect of parental lines and hybrids was also found to be non significant except 2.5 % span length (mm). From this study it can be concluded that there is no need of differentiating seeds of parental lines into superior, model and ordinary bulks for maintaining genetic purity. The electrophoretic patterns of seed proteins revealed a total of 38 bands for 4 parental lines and their 3 bulks. Out of which 2 bands (Rm values 0.38 and 0.62) were common to all genotypes except RHCb-001. The number of bands in each parental line ranged from 14 to 18 for seed proteins. Each parental line can be differentiated from other with respect to presence or absence of particular band.
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