Association analysis for yield related traits and foliar disease resistance using transposon specific markers in a mutant population of groundnut
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Abstract
An effort was made to identify association of transposable element (TE)-specific markers with
productivity and nutritional traits in a mutant population consisting of thirty five mutants and their
parents in groundnut. Field evaluation of the genotypes during kharif 2012 showed significant
genotypic differences and considerable variability for majority of the traits.
Genotyping of mutant population with 47 TE markers showed very high genotypediscriminating
power in terms of polymorphism information content (PIC) for a large proportion of the
markers. The overall polymorphism across mutant-parent pairs was 27.7%. Highest polymorphism of
72.3% was observed between Spanish Improved and TG 1. Three mutants, TG 2, TGM 8 and TGM
58 failed to show any polymorphism for the markers tested when compared to their parent (Spanish
Improved).
Marker-trait association was analysed by single marker analysis. Markers exhibiting
significant association with number of pods/plant, pod yield/plant, shelling percentage, sound mature
kernel weight (SMKW), test weight and disease resistance were identified. AhTE324 (44.41%),
AhTE305 (23.55%), AhTE372 (18.00%), AhTE491 (10.52%), AhTE373 (22.80%), AhTE130 (11.85%)
and AhTE491 (11.85%) showed the highest R2 (phenotypic variance explained) towards number of
pods/plant, test weight, resistance to LLS resistance and rust, pod yield/plant, shelling percentage
and SMKW, respectively. The markers identified as significantly associated with the traits were
validated by employing the independent mutants with phenotypic change in positive and negative
direction, but no evidence in favour of marker-trait association could be observed.
Mutants with unusual phenotypes like disease mimicking leaves, mosaic leaves, fused
leaflets and crinkle leaves, and long and narrow leaves were observed. However, these phenotypes
did not show any significant association with any of the markers tested. Hence, the markers identified
in this study as associated with important yield related traits and resistance to LLS and rust need to
be validated across other genotypes before employed for groundnut breeding.
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