The present investigation was conducted during the summer season of 1998 at the Instructional-Cum-Research Farm, Department of Horticulture, Mahatma Phule Krishi Vidyapeeth, Rahuri, Dist. Ahmednagar (Maharashtra) with a view to standardise the optimum dose of fertilizers for bitter-gourd RHRBG-3 under Rahuri conditions. The fertilizer treatments consisted of four levels of nitrogen (100,150, 200, 250 kg/ha) and two levels each of phosphorus and potassium (50, 100 kg/ha). Thus, there were 16 treatment combinations replicated three times in Factorial Randomized Block Design. The results obtained in respect of vine growth, yield, fruit quality and plant nutrient status as influenced by various treatments are summarised below. The results obtained in respect of vine growth, fruiting and yield response as influenced by various treatments revealed that the increase in growth with different levels of N was not linear. However, the higher dose (250 kg/ha) of nitrogen increased the growth of vine. The lower lev el (50 kg/ha) of phosphorus and higher level of potassium (100 kg/ha) increased the growth of vine. The yield attributing characters such as number of female and male flowers and fruit weight were significantly increased by the all four levels of nitrogen, the maximum being at 250 kg N/ha. The maximum number of fruits and total yield per plot and per hectare were obtained when 250 kg nitrogen, 50 kg phosphorus and 100 kg potassium per hectare were applied. The maximum fruit yield was recorded when N, P2O5 and K2O were applied at 250:50:100 kg/ha. he plant nutrient status in respect of N, P2O5 and K2O was increased with application of respective element but the bitter gourd hybrid did not show a better response to N, P2O5 and K2O fertilization proportionately. The optimum dose worked out was the combination of 250 kg N, 50 kg P2O5. and 100 kg K2O per hectare under the soil arid climatic conditions of Rahuri for bitter gourd RHRBG-3.