Biochemical and Molecular Characterization of Castor (Ricinus communis L.) Genotypes
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Date
2013
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Publisher
AAU, Anand
Abstract
Castor (Ricinus communis L.) is an important non-edible oil seed crop of Euphorbiaceae family. Castor is mainly grown in arid and semiarid regions. India, Brazil and China are major castor growing countries in the world. India ranks first in castor production. Gujarat is the largest castor producing state of our country. Mehsana and Banaskatha are the largest castor producing districts of Gujarat. Castor is grown for dry land farming. Castor is non-edible crop because of the presence of toxin named ricin.
The present study focuses on the approach to characterize castor genotypes using morphological, biochemical and molecular markers. In the study, seventeen castor genotypes were evaluated. Sixteen morphological traits, thirteen SSR primers and 8 ISSR primers were used to characterize the genotypes. Proximate composition was also studied for the different castor genotypes. SDS-PAGE analysis was done to study the protein banding. For SDS-PAGE (Sodium dodecyl sulphate polyacryalamide gel electrophoresis) analysis, 7DAG (Days after germination) seedlings were used to extract protein. For molecular analysis, DNA was extracted from young leaves of the standing crop.
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Keywords
agriculture, biotechnology, CHARACTERIZATION