" STUDIES ON MICROPROPAGATION IN AONLA {Emblica officinalis Gaertn.) "
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Date
2004-05-05
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MAHATMA PHULE KRISHI VIDYAPEETH RAHURI - 413722, AHMEDNAGAR, MAHARASHTRA, INDIA
Abstract
The investigation "Studies on micropropagation in aonla {Emblica officinalis Gaertn.)" was carried out to tandardize explant and composition of nutrient media for shoot regeneration and callus growth of aonla. The investigation comprised of various explants from two different sources viz., in vivo grown seedling and mature tree. The explants such as shoot tip, nodal segment, internodal segment, leaf, cotyledonary leaf, hypostem and root tips were evaluated from in vivo grown seedlings while nodal egments from mature trees of cv. Krishna were used. The plant culture media like Murashige and Skoog (MS) and Woody Plants Medium (WPM) were fortified with growth regulators (BAP, NAA, IAA, IBA and TDZ) in different concentrations and combinations. The results were analysed by adopting completely randomised block design. The results revealed that, maximum (75.00 %) bud break with determinate shoots from the nodal segments was observed in culture medium, MS + BAP 5 mg/1 + IAA 0.5 mg/1 + AC 2 g/1; while both i.e. determinate as well as indeterminate shoots were observed in WPM + BAP 5 mg/1 + IAA 0.5 mg/1 + AC 2 g/1. The nodal segment explants IV Abstract contd... Ph.D. J.H. Kadam excised from middle portion i. e. 11 to 15th node of indeterminate shoot and inoculated in the month of October showed maximum (76.66 %) bud break followed by those inoculated in the month of May (66.66 %). To reduce the phenolic browning, incorporation of AC 2 g/1 in culture media was the best treatment. The inborn contamination observed after the inoculation was reduced by treating the nodal segment explants with 0.1 per cent HgCl2 for five minutes. However, the shoots obtained upon subculture failed o establish roots. In the micropropagation studies of in vivo grown seedling, the nodal segment explants showed better response than shoot tip. The maximum shoot regeneration (88.33 %), earliest initiation (9.00 days), maximum height (4.00 cm) and maximum total number of leaves (7.67) were recorded in the treatment MS + BAP 0.25 mg/1 + NAA 0.1 mg/1 while more number of multiple shoots per explant (13.67) was observed with addition of GA3 2 mg/1. The nodal segment explants recorded 4.1 times more multiple shoots than shoot tip explants. The abnormal (spongy and thick) roots were observed in half strength MS medium supplemented with IBA and NAA, 2 mg/1, each. These plants when transferred for hardening failed to establish. In an attempt to propagate plantlets of aonla by intervention of callus, among the different explants tried the leaf explant showed profuse callus growth. The green and compact callus was obtained in the medium MS + NAA 2.5 mg/1. The proliferated callus when subcultured on regeneration medium, it proved recalcitrant.
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economics, biological phenomena, resins, productivity, technological changes, exhibitions, developmental stages, marketing, biological development, selection