DIVERSITY ANALYSIS IN SUGARCANE USING SOLUBLE PROTEIN PROFILES, ISOZYME PATTERNS AND RAPD MARKERS
Loading...
Files
Date
2004-12-09
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
MAHATMA PHULE KRISHI VIDYAPEETH, RAHURI - 413 722, DIST.AHMEDNAGAR, MAHARASHTRA, INDIA
Abstract
The genus Saccharum comprises of three cultivated species S. officinarwn, S. barberi and S. sinense and two wild species S. spontaneum and S. robustum. The present investigationentitled "Diversity analysis in sugarcane using soluble proteinprofiles, isozyme patterns and RAPD markers" was undertakenwith a view to analyse the diversity within cultivated varieties andamong the four species. The genetic elationship among Saccharumspecies and cultivated varieties was assessed using solubleproteins, two isozyme systems and fourteen RAPD markers. Theelectrophoretic spectra of soluble proteins from 11 species, whenresolved on 10 per cent polyacrylamide gel, exhibited seventy one bands of which sixteen were distinct polypetide bands with theXV11Abstract contd.... S.M. Bansoderelative mobility ranging from 0.1 to 0.9 and exhibiting both theuniformity as well as diversity of pattern between species and varieties. The similarity indices (SI), indicating evolutionary affinitybetween the species, ranged from 0.15 to 0.83. Comparison of thesimilarity indices between the cultivated varieties showedsignificant differences with a minimum similarity index value of0.33 between CO-95020 and CO-86032 and a maximum similarityindex value of 0.80 between CO-740 and CO-86032. Variabilityamong Saccharwn species and cultivated varieties was also examined which revealed detectable variation in the number andfrequency of isoenzymes. Peroxidase diversity among seven commercial hybrids quantified in terms of similarity indices showed variation, which ranged from 0.20 between CO-86032 and CO-94012 to 0.66 between in CO-95020 and CO-95008. The SI valueswhen compared between species showed variation from 0.20between S. officincurum and S. spontaneum to 0.66 between S. offtcinarum and S. sinense. Among the seven cultivated varieties, alate maturing variety, CO-740 exhibited only one band, whereasthe early maturing variety, CO-94012 showed 5 distinct bands foresterase. RAPD analysis exhibited a primer specific amplificationprofile. A minimum of 25 amplicons with the OPE-09 primer across7 cultivated varieties of sugarcane and a maximum of 67 amplification bands with OPE-03 primer were observed. Fourteenprimers showing amplification with almost all the eleven specieswere tested and the reproducibility confirmed. The averagesimilarity index between species, based on average of 14 primers,xviiiAbstract contd.... S.M. Bansoderanged from 0.52 to 0.82. A binary matrix of all the bands presentin each species was generated and the pairwise genetic similaritiesbetween the species under study were determined using Win Bootcomputational analysis and the dendogram was constructed. Theconsensus tree constructed of varieties clearly showed threedistinct groups. The late and midlate maturing varieties, CO-740,CO-86032 and CO-8014, formed a separate cluster; whereas three early maturing and high sugared varieties showed another cluster.Two varieties viz., CoC-671 and CO-94012 with commonpercentage (Q-63 x CO-775) were grouped under one cluster. TheRAPD results in comparison with the protein electrophoresisrevealed more discriminatory power as evident from polymorphicamplification profile among species. The study demonstratedusefulness of both the soluble protein electrophoresis and RAPDanalysis for diversity studies. Use of more isozyme system (> 5) andmore number of random primers further needs to be attempted.Use of more isozymes, RAPD markers and varieties belonging to distinct groups economic characters will help us to know the extentof diversity and identification of trait specific markers.
Description
Keywords
sowing, intercropping, wheats, tillage equipment, seed drilling, crops, mustard, fertilizers, land resources, chickpeas