"EVALUATION OF BIOCHEMICAL CONSTITUENTS IN GRAINS, LEAF NITRATE REDUCTASE POTENTIAL AND ELECTROPHORETIC PATTERNS OF SOLUBLE SEED PROTEINS IN MOTHBEAN GENOTYPES"
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Date
2005-06-24
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MAHATMA PHULE KRISHI VIDYAPEETH, RAHURI - 413 722, DIST. AHMEDNAGAR. MAHARASHTRA, INDIA
Abstract
The present investigation aims at evaluating the proximate composition of grains of twenty mothbean genotypes obtained from the Principal Scientist and Project Co-ordinator, AICRP on Arid Legumes, CAZRI, Jodhpur and Principal Scientist, Pulses Improvement Project of this University. The enzyme nitrate reductase was characterized from the trifoliate leaves of 20-dayold mothbean seedlings by an in vivo method. The nitrate reductase potentials in the leaves of some of these mothbean genotypes were also evaluated and correlated with their grain protein contents. Further, the electrophoretic patterns of soluble seed proteins of these genotypes were examined in order to assess divergence or relatedness between these genotypes. The grains of mothbean genotypes were analyzed for various biochemical constituents viz., moisture, ash, crude fat, crude protein, total carbohydrates.ash, minerals, phosphorus and calcium and limiting amino acids, methionine and tryptophan. All the genotypes differed ignificantly in their ( * XV Abstract Contd.. Miss S. S. Kale [(8.573-10.300 %), crude protein (18.660-25.958 %), crude fat (0.813-1.193 %), carbohydrates (57.330-63.557 %), ash (3.907-4.180 %), calcium (200.00-249.00 mg 100"1 g) and phosphorus (320.030- 358.970 mg 100"1 g), methionine (0.212- 0.419 g 16'1 g N) and tryptophan (0.749-1.145 g 16"1 g N). The infiltration media (5.0 ml) components used for the determination of in vivo NR activity for plus and minus KNO3 assays in mothbean trifoliate leaves were standardized in respect of nitrate, 40 mM; n-propanol alone, 3 % v/v; n-propanol, 1 %, v/v and Triton-X-100, 0.05 %, v/v together with sodiumphosphate buffer (100 mM, pH 7.5). Nitrate reductase enzymic potential in some of these genotypes was also assessed and correlated with their grain protein content. A significant positive correlation was observed between grain proteincontent and NR activity in trifoliate leaves of these mothbean genotypes. The seed soluble proteins in twenty mothbean enotypes were resolved by SDS-PAGE which exhibited a maximum of 15 bands with differences in banding pattern as far as intensity and molecular weights are concerned. In the soluble seed proteins of genotype MBS-27, an intense band, band No. 2 seems to be a characteristics of this genotype which could be used as a marker in its identification. A close phylogenetic relationship appears to exist in the genotypes, CZM-105 and PMB-3-2 as is evident from the presence of an uniqueband, band No. 5. This investigation thus, demonstrates genotype specificprotein polymorphism in mothbean grown under different geographical areas.
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sowing, intercropping, wheats, tillage equipment, seed drilling, crops, mustard, fertilizers, land resources, chickpeas