EVALUATION OF PROXIMATE COMPOSITION OF GRAINS AND LEAF NITRATE REDUCTASE ACTIVITY IN BLACK GRAM ( Vigna mungo (L.) Hepper) CULTIVARS
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Date
1999-09-15
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MAHATMA PHULE KRISHI VIBYAPEETH, RAHURI - 413 722, DIST. AHMEDNAGAR, (M.S.)
Abstract
The present investigation aims at evaluating the proximate composition of grams of ten pre-released and released black gram cultivars In addition, the nitrate reductase (NR) activity from the trifoliate eaves of black gram seedlmgs was standardized by an in vivo method The genetic potential of NR enzyme in the leaves of black gram cultivars was also examined and correlated with gram protem contents Further, it was desired to know the distribution of NR activity m different plant parts such as eaves, stems, petioles, roots and root nodules in 45-day-old black gram, cv TPU-4 The black gram seedlings and plants were grown in soil-filled pots under natural daylight conditions during the months from May to July, 1999 XV Abstract Contd.... D.M.R.S. Reddy The proximate analysis of grams of black gram cultivars indicated a range of 17 93 to 29 60 per cent of crude protein, 1 05 to 1 89 per cent of crude fat, 2 55 to 3 39 per cent of crude fibre, 2 52 to 4 12 per cent of total ash and 53 23 to66 65 per cent of carbohydrates The content of limiting amino acids such as methionine and tryptophan varied from 0 69 to 0 93 g/ 16 g N and 0.32 to 0 80 g/ 16 g N, respectively. The phosphorus content of the grams also exhibited a wide variation of 213 71 to 324 79 mg/100 g Iron, manganese and zinc content of the black gram grams vaned from 6 75 to 11 61 mg/100 g, 7 28 to 12.28 mg/100 g and 3 32 to 4.98 mg/100 g, respectively The infiltration media (5 0 ml) for determination of m vivo NR activity for plus KN03 assays m black gram trifoliate leaves was standardized as nitrate 100 mM, n-propanol (1%, v/v) and Tnton-X-100 (0.05%, v/v) together with sodium phosphate buffer (0 1 M, pH 7 5) The diurnal rythms were observed in the mtrate reductase enzyme activity m the trifoliate leaves of black gram The average NR activity which was low at 6.00 am m the morning mcreased rapidly as the day advanced, until it eached a maximum level at 2 00 p m. The NR activity, however, declined during the afternoon hours, reaching to a mimmal level at 6.00 p.m The evaluation of the potential of the NR enzyme from leaves mdicated that the highest NR activity was observed in the cultivar PU-9002-15, while the lowest was m BDU-1 The significant positive correlation between NR activity m leaves and protem content m the grains of the black gram cultivars mdicated that the cultivars XVI Abstract Contd.... D.M.R.S. Reddy with higher protem content and NR activity such as PU-9002-15 and TAU-2 could be selected for breedmg high protem genotypes The highest NR activity m 4 5-day-old black gram plants was observed in petioles, mdicatmg that petioles are the major site of nitrate assimilation m black gram, followed by leaves, stems, roots and root nodules m that order
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