“EVALUATION OF MOTH BEAN GENOTYPES FOR PROTEIN FRACTIONS AND SOME ANTINUTRITIONAL FACTORS”
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Date
2007-05-15
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MAHATMA PHULE KRISHI VIDYAPEETH, RAHURI- 413 722, DIST.AHMEDNAGAR, MAHARASHTRA, INDIA
Abstract
The present investigation entitled, " Evaluation of moth bean genotypes for protein fractions and some antinutritional factors" aims at evaluating the grains of twenty moth bean genotypes obtained from the Principal Scientist and Project Co-ordinator, AICRP on Arid Legumes, CAZRI, Jodhpur and Senior Pulses Breeder, Pulses Improvement Project o this University for crude protein content and some tinutritional factors. In addition, the proteins of the three high- and three low- proteins genotypes were separated into various solubility fractions. All the genotypes under study varied considerably in their crude protein content varying from 18.46 per cent in RMO-257 to 25.74 per cent in CZM-45 with a mean value of 2.53 per cent. The grain proteins of three high- (with protein content from 24.53 -25.74 per cent) and three low- (with xii Abstract Contd.... Miss. Shelar R. A. protein content from 18.46-21.13 per cent) protein enotypes on fractionation revealed that the albumin (water soluble fraction) was the major fraction comprising of 42.79-51.59 per cent of total proteins, followed by globulin, prolamine and glutelin. uther, the results revealed that genotypes with high crude protein content in grains exhibited low per cent protein recovery as compared to low protein genotypes which showed high protein recovery. Among the antinutritional factors, the polyphenol content in the grains of these moth bean genotypes varied considerably ranging from 0.24 to 0.46 per cent, which appears to be appreciably low as compared to the values reported by other researchers in the literature. On account of low polyphenol content, the moth bean seeds are thus anticipated to have better nutritive value than other pulses. The phytate phosphorus ranged from176.49-218.91 mg 100"'g with a mean value of 198.26 mg 100"'g and constituted approximately 59 per cent of the total phosphorus. The trypsin and a-amylase inhibitor activities also varied from 162.41-201.48 units g'1. and 80.46-97.67 units g"1 with a mean value of 182.88 units g"1 and 89.56 units g"1, respectively. Based on the results obtained in the present investigation, the genotype CZM-45 appeared to have comparatively low level of antinutritional factors than CAZRI MOTH-1 besides having high
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Agricultural Biochemistry