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EVALUATION AND UTILIZATION OF SSR MARKERS FOR GENETIC DIVERSITY CHARACTERIZATION IN SEABUCKTHORN.
Sharma, Ram Kumar
2019-07
M.Sc
Seabucktthorn (Hiippophae rhamnoiides,, diiplloiid 2n=24) iis a diioceous,, wiind polllliinatted,, tthorny shrub bellongs tto famiilly Ellaeagnaceae.. In Indiia,, iitt grows natturalllly iin hiigh allttiittude and colld-ariid regiions of Ladakh,, Siikkiim,, Jammu & Kashmiir,, Hiimachall Pradesh,, and Uttttrakhand.. Itt iis economiicalllly and ecollogiicalllly iimporttantt mediiciinall pllantt due tto iitts hiigh iimmense mediiciinall properttiies butt tthere iis unavaiillabiilliitty of superiior variietty// sellecttiion avaiillablle for llarge scalle commerciiall cullttiivattiion iin Indiia.. Furtthermore,, valliidatted miicrosattelllliitte marker resources are allso lliimiitted iin tthiis pllantt speciies.. Therefore,, a ttottall of 882 novell SSR markers were miined from iin-house H.. rhamnoiides ttranscriipttome datta.. Among allll,, abundance of ttrii-repeatts (49%) follllowed by dii-repeatts (46%) was observed.. Furtther,, evalluattiion and uttiilliizattiion of pollymorphiic SSR makers for genettiic diiversiitty assessmentt of 245 accessiions of Seabucktthorn (H.. rhamnoiides) dettectted hiigh average allllelle frequency (5..45 allllelles// priimer) and hiigh hetterozygosiitty (Ho:: 0..77;; He:: 0..75).. Neiighbor-Joiiniing dendrogram,, priinciipall coordiinatte anallysiis (PCoA) and Bayesiian modell based popullattiion sttructture anallysiis grouped allll tthe 245 iindiiviidualls iintto ttwo majjor groups wiitth hiigh wiitthiin popullattiion gene fllow suggesttiing tthatt tthere are ttwo genettiic poolls operattiing iin tthe natturall seabucktthorn popullattiions of ttrans--Hiimallayan Leh and Keyllong regiions of Indiia.. The resulltts of tthiis sttudy wiillll be usefull for iidenttiifiicattiion and sellecttiion of pottenttiiall genottypes tto expediitte tthe conservattiion and genettiic iimprovementt programme iin seabucktthorn.. Genome-wiide miicrosattelllliitte marker resource creatted duriing tthiis sttudy can be valliidatted and uttiilliized for futturiisttiic evolluttiionary and variious genottypiic sttudiies iin seabucktthorn.
SEABUCKTHORN
en
61
CSKHPKV, Palampur
Agricultural Biotechnology
Utilization off SSR markers for genetic diversity characterization in Seabuckthorn.
Thesis

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