NUTRITIVE EVALUATION OF UNCONVENTIONAL FEEDS USING IN VITRO GAS PRODUCTION TECHNIQUE
Ally K
2016
M.V.Sc.
A study was conducted to evaluate the sixteen locally available unconventional feeds such as mulberry leaves (Morus sp), brewery waste, rain tree pod (Samanea saman), rapeseed cake (Brassica napus), glyricidia leaves (Glyricidia sepium), azolla (Azolla pinnata), bamboo leaves (Bambusoideae), turmeric waste (Curcuma longa), spent cumin (Cuminum cyminum), cooked barley residue (Hordeum vulgare), water hyacinth (Eichhornia crassipes), soya sauce waste (Glycine max), dhanwantaram thailum residue, pineapple tops (Ananas comosus), spent rosemary (Rosmarinus officinalis) and spent grapes (Vitis vinifera) using in vitro gas production technique. Feed samples were analysed for their proximate composition. Based on the crude protein (CP) levels, these sixteen samples were grouped into three. Group I (G1) - Unconventional feeds with more than 20 per cent CP (Mulberry leaves, brewery waste, rain tree pod meal, rapeseed cake, glyricidia leaves and azolla). Group II (G2) - Unconventional feeds with more than 10 per cent CP (Bamboo leaves, turmeric waste, spent cumin, cooked barley residue, and water hyacinth). Group III (G3) - Unconventional feeds with less than 10 per cent CP (Dhanwantaram thailum residue, pineapple tops, spent rosemary and spent grapes). The samples were subjected to in vitro trials according to the procedure described by Menke and Steingass (1988) to estimate various rumen fermentation parameters such as total in vitro gas production (IVGP), in vitro true dry matter/organic matter digestibility (TDMD, TOMD), rumen ammonia nitrogen concentration, and individual VFAs and to calculated parameters like partitioning factor (PF), microbial biomass production (MBP), metabolisable energy (ME). The level of various nutrients ranged from 16 to 83 per cent for moisture, crude protein (CP) ranged from 5.61 to 34.21 per cent, and other nutrients such as ether extract (EE), crude fibre (CF), total ash (TA) and neutral detergent fibre (NDF) ranged from 0.41 to 17.7, 6.28 to 31.33, 2.14 to 26.66 and 34.31 to 60.41 per cent respectively. The average gas production (ml/200mg DM) ranged from 9.85 to 56.75. Highest gas production was recorded in cooked barley residue and lowest in azolla. The average true matter digestibility ranged from 49.18 to 87.05 and true organic matter digestibility ranged from 49.10 to 86.94 per cent. The cumulative gas production values (up to 24 h) and values of other chemical constituents (CP, EE, and TA) were used to arrive at the metabolisable energy content of the feedstuffs evaluated, followed by partitioning factor and microbial biomass production. Individual volatile fatty acids (mmol/dL) such as acetate, propionate, butyrate and valerate were estimated using gas chromatography. Acetate production ranged from 22.54 to 47.62, propionate was lowest in soya sauce waste (7.91) and highest in cooked barley residue (22.78). Butyrate ranged from 2.15 to 5.47 and acetate: propionate ranged from 1.6 to 2.97. Ammonia nitrogen (mg/dL) was highest in rapeseed cake (39.69) and lowest in mulberry leaves (24.10).
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSUR
NUTRITIVE EVALUATION OF UNCONVENTIONAL FEEDS USING IN VITRO GAS PRODUCTION TECHNIQUE
Animal Nutrition
NUTRITIVE EVALUATION OF UNCONVENTIONAL FEEDS USING IN VITRO GAS PRODUCTION TECHNIQUE
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