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Central Agricultural University, Pasighat

The Central Agricultural University has been established by an act of Parliament, the Central Agricultural University Act, 1992 (No.40 of 1992). The Act came into effect on 26th January, 1993 with the issue of notification by the Department of Agricultural Research and Education (DARE), Govt. of India. The University became functional with the joining of first Vice-Chancellor on 13th September, 1993. The jurisdiction of the University extends to six North-Eastern Hill States viz., Arunachal Pradesh, Manipur, Meghalaya, Mizoram, Sikkim and Tripura, with headquarters at Imphal in Manipur. College of Horticulture & Forestry, a constituent College of the Central Agricultural University, Imphal, Manipur, was established on 7th March 2001 at Pasighat, Arunachal Pradesh on the bank of beautiful river Siang.

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Author: Daryal, Unshani ×
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    ThesisItemOpen Access
    Analysis of variability in morphological and molecular characteristics of Melia dubia Cav.Syn. Melia composita Willd
    (College of Horticulture and Forestry, Central Agricultural University, Pasighat, 2021-03-04) Daryal, Unshani; Dobhal, Shivani; Mehra, Tara Singh
    In the present study, ANOVA using CRD was conducted which revealed significant differences among the selected plus trees for all characters studied. The highest GCV and PCV was observed as 29.19 and 31.31 respectively. The GCV is found to have lower magnitude than the PCV for all the traits. This implies that these traits are influenced by the local environmental factors. Heritability varied from 87% to 98%, the first 4 traits have high heritability values accompanied with high genetic advance which indicates strong additive gene action. High heritability but low genetic advance was observed in pulp thickness which indicate presence of non-additive gene effects and high environmental interaction. Themaximum inter-distance(4943.48) was found between cluster-3 and cluster-4. The minimum inter-cluster distance(501.93) was recorded between cluster-2 and cluster-4 indicating genetic closenesss. The maximum intra-cluster distance(164.21) was found in cluster-1 and the minimum was found in cluster-5 with an intra-cluster distance zero. The maximum intra-cluster distance shown by cluster 1 implies greater genetic diversity than other clusters. The 34 plus trees were grouped into five clusters through cluster analysis. Cluster 1 had a maximum number of CPTs with 26 genotyps, whereas the least number observed for cluster 5 with just 1 genotype. Clusters with only one plus tree can either possess superior or inferior quality among the plus trees. The clustering pattern indicates that plus trees from the same geographic sources were grouped into different clusters and vice-versa. PCA of the morphological characters revealed the first three components have the eigenvalues greater than one and cumulatively accounted for 94.8% of the total variation. The protocol of genomic DNA extraction by Swati et al. (2016) as described by Doyle and Doyle, (1987) was used in the present study.The concentration of genomic DNA from juvenile leaves of Melia dubia with absorbance ratio (A260/A280) ranged from 1.71 to 2.30. The highest average expected genetic diversity (h) (0.47) was obtained with primer C31 and lowest (0.35) with primer MAC69.The highest polymorphism (PIC) was reported with primer C31 (0.48) and lowest (0.35) with primer MAC69. The selected plus trees were amplifiedusing 15 SSR markers, of which 5 primers were found to be polymorphic. The UPGMA dendrogram was generated, which resulted in the formation of 8 clusters, where the biggest cluster was found in cluster 1 with 15 different genotypes. The genetic dissimilarity between pair-wise comparisons of the genotypes using NTSYS. The overall dissimilarity values among the selected plus trees ranged from 0.00 to 0.85. Though overall correlation between matrices was low (r=-0.04426, p=0.2212), both morphological and molecular dendogram clustered various progenies on least similar patterns.
  • Thumbnail Image
    ThesisItemOpen Access
    Analysis of variability in morphological and molecular characteristics of Melia dubia Cav.Syn. Melia composita Willd.”
    (College of Horticulture and Forestry, Central Agricultural University, Pasighat, 2021-03-04) Daryal, Unshani; Dobhal, Shivani; Taying, Tisu
    In the present study, ANOVA using CRD was conducted which revealed significant differences among the selected plus trees for all characters studied. The highest GCV and PCV was observed as 29.19 and 31.31 respectively. The GCV is found to have lower magnitude than the PCV for all the traits. This implies that these traits are influenced by the local environmental factors. Heritability varied from 87% to 98%, the first 4 traits have high heritability values accompanied with high genetic advance which indicates strong additive gene action. High heritability but low genetic advance was observed in pulp thickness which indicate presence of non-additive gene effects and high environmental interaction. Themaximum inter-distance(4943.48) was found between cluster-3 and cluster-4. The minimum inter-cluster distance(501.93) was recorded between cluster-2 and cluster-4 indicating genetic closenesss. The maximum intra-cluster distance(164.21) was found in cluster-1 and the minimum was found in cluster-5 with an intra-cluster distance zero. The maximum intra-cluster distance shown by cluster 1 implies greater genetic diversity than other clusters. The 34 plus trees were grouped into five clusters through cluster analysis. Cluster 1 had a maximum number of CPTs with 26 genotyps, whereas the least number observed for cluster 5 with just 1 genotype. Clusters with only one plus tree can either possess superior or inferior quality among the plus trees. The clustering pattern indicates that plus trees from the same geographic sources were grouped into different clusters and vice-versa. PCA of the morphological characters revealed the first three components have the eigenvalues greater than one and cumulatively accounted for 94.8% of the total variation. The protocol of genomic DNA extraction by Swati et al. (2016) as described by Doyle and Doyle, (1987) was used in the present study.The concentration of genomic DNA from juvenile leaves of Melia dubia with absorbance ratio (A260/A280) ranged from 1.71 to 2.30. The highest average expected genetic diversity (h) (0.47) was obtained with primer C31 and lowest (0.35) with primer MAC69.The highest polymorphism (PIC) was reported with primer C31 (0.48) and lowest (0.35) with primer MAC69. The selected plus trees were amplifiedusing 15 SSR markers, of which 5 primers were found to be polymorphic. The UPGMA dendrogram was generated, which resulted in the formation of 8 clusters, where the biggest cluster was found in cluster 1 with 15 different genotypes. The genetic dissimilarity between pair-wise comparisons of the genotypes using NTSYS. The overall dissimilarity values among the selected plus trees ranged from 0.00 to 0.85. Though overall correlation between matrices was low (r=-0.04426, p=0.2212), both morphological and molecular dendogram clustered various progenies on least similar patterns.