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Birsa Agricultural University, Ranchi

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  • ThesisItemOpen Access
    PROPAGATION AND GROWTH PERFORMANCE OF GERMPLASM OF Tinospora cordifolia (Willd.) Hook. f. & Thoms. from JHARKHAND
    (Birsa Agricultural University, Ranchi, 2019) Kumar Ranjan, Dinesh; Kumar, Kaushal
    Tinospora cordifolia (Willd.) Hook. f. and Thoms. Commonly known as ‘Giloy’ belonging to family Menispermaceae and their Ayurveda names are ‘Guduchi’, ‘Amrita’ etc. It is a diverse, large, deciduous climbing shrub with greenish yellow typical flowers, with corky, grooved bark with adventitious aerial root with simple alternate leaves. Stem of this plant is rather succulent with long, filiform, fleshy and climbing in nature, bark is creamy white to grey, and aerial roots are long filiform, threadlike. It is distributed throughout in tropical India subcontinent up to an altitude of 300m. The stem of the plant is high valued for potential medicinal properties for the treatment of fever, diabetes , arthritis, inflammation and as anti-oxidant, anti-allergic, anti-stress, anti-malarial, immunomodulatory etc. The studies have been carried out with following Objectives: 1) To study plant morphology of ‘Giloy’ collected from different Agro- climatic zones in Jharkhand. 2) To evaluate growth performance of the above collected plants samples (germplasm) cultivated through stem cutting. 3) To estimate ‘Giloy - Satva’ from the stems of plants. Stem cuttings are the best planting material for raising commercial crop. The plant can be grown in almost all climates but prefers warm climate. It grows well in almost any type of soils but medium black soil or red soil is the best for the cultivation of Tinospora cordifolia. The plant can be propagated by seeds and also stem cuttings which is the best and suitable methods. The cuttings of the small finger thickness with 6 to 8 inch length of long stem having two nodes are used. This species can grow in organic manures like Farm Yard Manure (FYM), Vermi- Compost, Green Manure etc. as per requirement of the species. The field after plantation should be irrigated periodically mostly required weekly. Mature stems of the plants are collected, cut into small pieces and dried in shade and processed for obtaining ‘Giloy-satva’ which are utilized commercially in herbal Industry. There are several products in the market based on Giloy are Giloy- Ghanvati, Giloy Powder, Giloy Juice, Giloy Capsule etc.
  • ThesisItemOpen Access
    Studies on impact of organic and inorganic fertilizers on growth and yield of Ashwagandha [Withania somnifera (L.)Dunal]
    (Birsa Agricultural University, Ranchi, 2019) ROUTRAY, AISHWARYA; Kumar, Kaushal
    Ashwagandha [Withania somnifera (L.) Dunal] is a medicinal plant belongs to family Solanaceae. The roots of the plant is highly useful in the classical system of medicine mainly Ayurveda. There are a large of number of herbal product in ayurveda and other systems of medicine based on Ashwagandha due to their therapeutic, curative and potent action of pharmalogical activities. There are some active principles particularly somniferin, withanin etc, has found highly effective as potency, immuno-modulator and to treat the disability of nervous system. However, as per demand in the global market, there are less researches has been carried out on cultivation practices particularly for specific reason. In Jharkhand there are need of promotion of cultivation practices of Ashwagandha. Therefore, to understand the growth performances and yield, the present study has been attempted to “Studies on impact of organic and inorganic fertilizers on growth and yield of Withania somnifera (L.) Dunal (Ashwagandha)”. The applications of fertilizer doses have direct impact on productivity status of medicinal and aromatic plants. Standardization of fertilizer application is necessary for remunerative cultivation and one of the primary aims of scientific cultivation of medicinal & aromatic plants. Moreover, economics is directly related to input and output of production system. Therefore, optimization of fertilizer doses becomes important aspect for productivity estimation research activities. In Jharkhand, because of the presence of acidic soil micro and macro nutrients are less available to plant so the growths are being affected. The application of fertilizer is helpful to improve the physical and chemical properties of soil which are very much important to cope with different environmental factor and improve production and productivity.In some cases particularly in potassic fertilizers also helps to fight against different pest and diseases with the different plants under open condition are liable to pest.Keeping these facts, it is necessary to find suitable doses of fertilizers which will help the farmers to enhance their production and to get proper financial return from the crop with the following objectives:-i) to screen out optimum dose of organic and inorganic fertilizers on growth and yield of Ashwagandha.ii) to study the impact of fertilizer combination on reproductive parameters of the plant iii) to screen out selection indices having significant impact on main yield parameter.
  • ThesisItemOpen Access
    Genetic Divergence Analysis of Mucuna pruriens (L.) DC. through its Quantitative and Reproductive Traits
    (Birsa Agricultural University, Ranchi, 2019) THAKUR, ISHA; Kumar, Jai
    Assessment of genetic diversity through quantitative and qualitative traits in Mucuna pruriens germplasm is particularly important as the species is a self-pollinating crop and therefore vulnerable to environmental effects. No systematic information is available regarding the evaluation and screening of high yielding germplasm of Mucuna pruriens, hence screening of viable germplasm is essential for the recommendation of its commercial cultivation. Keeping in view the importance of divergence study in Mucuna pruriens a systematic research trial was undertaken to quantify the extent of divergence of different Mucuna pruriens germplasm, an experiment was conducted at AICRP (M&APs) farm, BAU, Ranchi with the following objectives: 1.To study the genetic diversity analysis among selected Mucuna pruriens germplasm 2. To screen out superior Mucuna pruriens germplasm on the basis of quantitative parameters 3. To screen out early, medium or late variety of Mucuna pruriens on the basis of reproductive parameters 4. To study the traits association pattern between different quantitative parameters of Mucuna pruriens. Research trial was laid out in Randomised Block Design with 24 treatments and 4 replications of Mucuna pruriens collected from 3 different places i.e. IIHR, DMAPR, Ranchi. Qualitative parameters studied were flower colour, bearing habit, colour of immature pod, leaf colour, seed coat colour, seed coat pattern, seed coat brilliance, pod shape, seed colour, seed shape, pod pubescence intensity. Quantitative parameters studied were Growth parameters: plant length, collar diameter, leaflet length, leaflet breadth, number of pods/bunch, number of seeds/pod, pod length, pod width, seed size (length, breadth); Reproductive parameters: Days to first flowering, days to 50% flowering, inflorescence length, no. of flowers/ inflorescence, days to complete pod maturity; Yield parameters: Weight of dried pods,100 seed weight, pod yield/plant, pod yield/ha, seed yield/plant, seed yield/ha; Biochemical parameters: L-Dopa content of seeds, L-Dopa yield/ha. Data collected on all quantitative traits were subjected to ANOVA. Genetic diversity of 24 germplasm of Mucuna pruriens was estimated by multivariate analysis of genetic divergence of Mahalanobis “D2” statistics. Relative contribution of different qualitative traits towards genetic divergence was calculated based on the magnitude of the D2 value due to each trait. All germplasms was grouped into a number of clusters, according to the methods described by Tocher. Data on different growth, reproductive, yield and biochemical parameters was also subjected to correlation analysis and path value analysis.
  • ThesisItemOpen Access
    Genetic divergence and traits association studies in Rauvolfia serpentina (L.) Benth. Ex Kurz (Sarpgandha)
    (Birsa Agricultural University, Ranchi, Jharkhand-6, 2018) BAKHLA, RASHMI; Kumar, Jai
    Keeping in view the importance of divergence study, a systematic research trial was undertaken to quantify extent of divergence of different Sarpgandha germplasm through its qualitative and quantitative traits at AICRP (M&APs) farm, BAU, Ranchi with the following objectives. 1. To estimate the extent of genetic divergence and clustering pattern among selected germplasm of Sarpgandha 2. To screen out superior germplasm of Sarpgandha on the basis of quantitative traits 3. To find out percentage contribution of quantitative traits towards its genetic divergence 4. To study trait association pattern with regards to yield to facilitate selection of traits 5. To determine heritability and genetic advance of quantitative traits useful for improvement. Research was laid out in RBD with 25 treatments replicated thrice. Experimental material consisted of 25 germplasm of Sarpgandha (BRS1 to BRS25) collected from different districts of Jharkhand. Different qualitative and quantitative parameters were measured as per NBPGR guidelines. The replicated data of all the 25 germplasm for quantitative traits was subjected to genetic divergence analysis, analysis of variance, correlation matrix, path value analysis and principal component analysis. 15 germplasm of Sarpgandha showed semi-erect plant growth habit, while 10 had erect. Three types of leaf arrangement were found namely verticilite (16), alternate (5) and opposite (4). The root colour of 17 germplasm was pale brown while 8 germplasm had grayish brown root colour. Two type of inflorescence were found namely corymbose (14) and umbellate (11). Flower colour of 11 germplasm was pink and rest 14 germplasm were white. The fruit shape of six germplasm was globose, 9 were ovoid and 10 were sub-globose. Two type of fruit colour were noticed, and out of 25 germplasm, 19 had dark purple colour and six had brownish colour. Seed shape of 13 germplasm was elliptical and 12 were obovate. Significant differences were noticed in the plant height and maximum value was recorded for BRS2 (67.66cm) and minimum by BRS20 (37.33). Significant differences were noticed in stem diameter and maximum value was noticed for BRS15 (11.63mm), and minimum for BRS4 (7.30mm). In case of number of primary branches/plant, maximum value was noticed for BRS1 (7.00), and minimum for BRS7 (2.33). Significant differences were observed for number of leaves/plant and its maximum value was recorded for BRS23 (69.33) and least by BRS20 (24.33). Significant difference was also observed for number of nodes/plant and maximum value was recorded for BRS2 (13.66) and minimum for BRS12 (7.66). Significant differences were observed in case of length of inter-nodes and its maximum value for recorded BRS8 (10.85cm) and minimum in BRS7 (6.42cm). Maximum crown spread in N-S and E-W directions was recorded for BRS7 (33cm and 30.66cm) respectively and its minimum value observed for BRS25 (19.16cm and 20.16cm) respectively. Significant differences were also observed in case of inflorescence length and maximum value observed for BRS16 (10.31cm) and minimum value BRS21 (6.24cm). Maximum leaf length was recorded for BRS25 (13.17cm) which varied significantly from its minimum value recorded for BRS6 (9.50cm). In case of leaf width significant difference was observed with its maximum value for BRS10 (4.65 cm) and minimum for BRS7 (3.06 cm). Significant difference was observed in the number of inflorescence/plant and its maximum value was recorded for BRS2 (13.66) and minimum for BRS6 (2.33). Number of flower/inflorescence was also varied significantly and its maximum value was recorded for BRS18 (94.66) and minimum for BRS7 (17.66). In case of number of fruits/inflorescence, significant differences were observed with its maximum value BRS18 (88.66) and minimum for BRS7 (12.66). Number of seeds/fruit was also varied significantly with its maximum value BRS2 (1.72) and minimum for BRS11 and BRS22 (1.05). Maximum root length was recorded for BRS2 (88.16 cm) which varied significantly with its minimum value of BRS20 (68.03 cm). Significant differences were observed in root diameter with its maximum value in BRS4 (17.31 mm) and minimum in BRS18 (13.05 mm). As regards to dry root yield/plant, maximum value was recorded for BRS9 (83.32 g) which varied significantly with minimum value recorded for BRS20 (42.85 g). In case of seed yield/plant, maximum value recorded for BRS23 (21.89 g) which varied significantly with its minimum value recorded for BRS5 (1.51 g). Among the sources of genetic diversity in Sarpgandha germplasm, maximum percentage of contribution was shown by seed yield/plant (51.33%) followed by inflorescence length (19.33%) and number of flower/inflorescence (11.67%). Seven clusters were formed through genetic divergence analysis, out of which cluster I contains maximum 9 number of germplasm. Maximum inter cluster distance was observed between cluster III and cluster VI (13.59) followed by cluster II and cluster III (12.46). Correlation matrix of different plant characters showed plant height had highly significantly positively correlated with dry root yield/plant (0.594) and significant positive correlation between root length and dry root yield/plant (0.414). Path analysis of different growth parameter affecting seed yield/plant indicated maximum direct positive impact by no. of flowers/ inflorescence (0.658) and maximum direct negative impact by no. of fruits/inflorescence (-0.486). In case of dry root yield/plant, maximum direct positive effect was shown by plant height (0.590) and maximum direct negative effect was by root diameter (- 0.472). Maximum heritability was shown by seed yield/plant (97.34%) followed by inflorescence length (88.42%). However, maximum genetic advance was also shown by seed yield/plant (129.13%) followed by no. of fruits/inflorescence (79.59%). So to conclude, as regards to estimate the extent of genetic divergence and clustering pattern among selected germplasm of Sarpgandha, seven clusters were formed, out of which cluster I consisted of 9 germplasm, followed by cluster II (8 germplasm) and cluster III (4 germplasm). Maximum inter cluster divergence was found between cluster III and cluster VI (13.59) followed by cluster II and cluster III (12.46) and cluster III and VII (10.44). So parents may be selected for hybridization program from these clusters i.e. cluster (III & VI), (II & III) and (III & VII). The extent of genetic divergence was ranged between 3 to 30% which may be considered as low genetic divergence. As regards to screen out superior germplasm of Sarpgandha on the basis of quantitative traits, on the basis of dry root yield/plant, three germplasm namely BRS9 followed by BRS23 & BRS5 may be selected as superior germplasm because they produced maximum 83.32, 78.25 & 77.97 g dry root yield/plant respectively, which was significantly superior to rest of the germplasm. On the basis of seed yield /plant, three germplasm namely BRS23 followed by BRS21 & BRS12 may be selected as superior germplasm because they produced maximum 21.89, 21.06 & 20.79 g seed yield/plant respectively, which was significantly superior to rest of the germplasm. As regards to find out percentage contribution of quantitative traits towards its genetic divergence, maximum genetic divergence was shown by seed yield/plant (51.33 %), inflorescence length (19.33%) & number of flowers /inflorescences (11.67 %). As regards to study trait association pattern with regards to yield to facilitate selection of traits, plant height showed high significant positive correlation with dry root yield/plant (0.594) & significantly positively correlated with root length (0.414). Again maximum direct positive effect on dry root yield/plant was shown by plant height (0.590), followed by number of leaves/plant (0.224) & root length (0.135). So plant height & root length may be selected as suitable traits for improving dry root yield/plant in Sarpgandha. As regards to determine heritability and genetic advance of quantitative traits useful for improvement, maximum heritability was shown by seed yield/plant (97.34%) followed by inflorescence length (88.42%) & number of fruits/inflorescence (83.28%). Maximum genetic advance was shown by seed yield/plant (129.13%) followed by number of fruits/inflorescences (75.59%).
  • ThesisItemOpen Access
    Propagation studies in Rauvolfia serpentina (L.) Benth. ex Kurz and Gymnema sylvestre R. Br. stem cuttings through PGRs
    (Birsa Agricultural University, Ranchi, Jharkhand-6, 2018) KUMARI, JYOTI; Kumar, Jai
    Keeping the importance of vegetative propagation in ensuring mass multiplication of seedlings particularly of those species, which are difficult to germinate through seeds and to multiply the seedlings of uniform genetic composition, identical to parental type transmission, a systematic research trial was conducted to know the effect of PGRs on growth, rooting and survival of stem cuttings of Sarpgandha and Gudmar at AICRP (M&APs) farm, BAU, Ranchi with the following objectives (a) To standardize the dozes of PGRs needed to obtain seedlings from stem cuttings of Rauvolfia serpentina and Gymnema sylvestre (b) To study the effect of different PGRs on rooting and shooting behavior of Rauvolfia serpentina and Gymnema sylvestre stem cuttings (c) To study the effect of PGRs on the survival of Rauvolfia serpentina and Gymnema sylvestre stem cuttings. Research was laid out in Factorial Completely Randomized Design with 13 treatments replicated thrice. Parameters studied were rooting percentage, number of roots/cutting (primary & secondary), length of the root (primary & secondary), sprouting percentage, number of sprouts/cutting, length of shoot, number of leaves/cutting, number of days taken for initiation of shoot emergence, number of days taken for completion of shoot emergence, basal diameter of shoot, area of leaves and survival percentage of cuttings. After two and half months from transplanting the cuttings into polythene tubes, data was collected for different root and shoot parameters and statistically analyzed to draw meaning inferences. Minimum number of days taken for initiation of shoot emergence in Sarpgandha was observed in 1000 ppm IAA and 1000 ppm NAA (2.33 days), while in case of Gudmar, it was 3.00 days in 7 PGRs treatments. Maximum number of days taken for completion of shoot emergence in Sarpgandha was observed in 250 ppm IBA (20.33 days), while in case of Gudmar, it was 16.00 days in 250 ppm IAA. Maximum rooting percentage (83.33 and 45.00%) was observed in Sarpgandha and Gudmar respectively under the treatment 750 ppm NAA and 750 ppm IBA. Maximum sprouting percentage of Sarpgandha was observed in 750 ppm NAA (86.67%), while in case of Gudmar, it was 61.67% in 750 ppm IBA, which was at par with 500 ppm IBA, 1000 ppm IBA and 750 ppm NAA. Maximum number of primary roots (3.20) in Sarpgandha cuttings was observed in 750 ppm IAA and in case of Gudmar; it was 3.43 in 750 ppm IAA, at par with 750 ppm IBA and 750 ppm NAA. Maximum number of secondary roots (119.73) in Sarpgandha cuttings was observed in 750 ppm IBA, and in case of Gudmar, it was 76.40 in 750 ppm IAA. Maximum length of primary roots (4.69 cm) in Sarpgandha cuttings was observed in 750 ppm IAA, which was at par with 750 ppm IBA and 750 ppm NAA and in case of Gudmar, it was 10.31 cm in 750 ppm IBA, which was at par with 1000 ppm IBA. Maximum length of secondary roots (1.75 cm) in Sarpgandha cuttings was observed in 750 ppm NAA, and in case of Gudmar, it was 2.23 cm in 750 ppm IBA. Maximum number of sprouts (2.29) in Sarpgandha cuttings was observed in 750 ppm IBA, and in case of Gudmar, it was 2.35 in 750 ppm IBA. Maximum length of shoots (4.60 cm) in Sarpgandha cuttings was observed both in 500 ppm IAA and 750 ppm IAA, and in case of Gudmar, it was3.05 cm in 750 ppm IBA. Maximum number of leaves (12.45) in Sarpgandha cuttings was observed in 750 ppm IBA, and in case of Gudmar, it was 17.52 in 750 ppm NAA. Maximum basal diameter of shoots (2.59 mm) in Sarpgandha cuttings was observed in 750 ppm NAA, which was at par with 9 PGR treatments and in case of Gudmar, it was 1.97 mm in 1000 ppm IBA. Maximum area of leaves (13.50cm2) in Sarpgandha cuttings was observed in 750 ppm NAA, which was at par with 6 PGR treatments and in case of Gudmar, it was 4.82 cm2in 750 ppm IBA, which was at par with 9 treatments. Maximum survival percentage (70.00%) in Sarpgandha cuttings was observed in 500 ppm NAA, which was at par 250 ppm NAA (65.00%) and 750 ppm NAA (66.67%) and in case of Gudmar, it was 45.00% in 750 ppm NAA, which was at par with 4 treatments. Sprouting trend in Sarpgandha cuttings indicates that maximum sprouting was occurred between 17-19 days. Average sprouting percentage under PGR treatment varied from 70-80%. Sprouting trend in Gymnema sylvestre cuttings indicates that maximum sprouting was occurred between 19-20 days. Average sprouting percentage under PGR treatment varied from 40-45%. Correlation matrix among traits of stem cuttings of Sarpgandha indicated that survival percentage is highly significantly positively correlated with 11 traits and among them maximum value is shown by sprouting percentage (0.962). In case of Gudmar, survival percentage is highly significantly positively correlated with 8 traits and among them maximum value is shown by number of leaves/cutting (0.949). Path analysis of traits affecting survival percentage of Sarpgandha cuttings indicated that six traits had direct positive effect, out of which maximum direct positive effect was shown by number of primary roots/cutting (2.120) and maximum direct negative effect (-1.878) was shown by number of secondary roots/cutting. Path analysis of traits affecting survival percentage of Gudmar cuttings indicated that nine traits had direct positive effect, out of which maximum direct positive effect was shown by number of leaves/cutting (0.810) and maximum direct negative effect (-0.942) was shown by length of secondary roots. Most of the traits having highly significant positive relation with survival percentage of Sarpgandha cuttings in 750 ppm NAA, while in case of Gudmar, it is with 750 ppm IBA. So, 750 ppm NAA and 750 ppm IBA is the standardized doze of PGR to obtain maximum seedlings from stem cuttings of Rauvolfia serpentina and Gymnema sylvestre respectively. Maximum rooting (83.33%) and sprouting percentage (86.67%) of Sarpgandha cuttings was observed under 750 ppm NAA treatment, while maximum rooting (45.00%) and sprouting percentage (61.67%) of Gudmar cuttings was observed under 750 ppm IBA treatment. 750 ppm NAA treatment is best for Sarpgandha as well as Gudmar cuttings for its maximum survival. It could be concluded that to obtain uniform, healthy and vigorous seedlings of Rauvolfia serpentina, stem cuttings of length 15 cm, thickness 6-7 mm, having 4-5 bud should be dipped up to 5 cm in 750 ppm NAA for two minutes and transplanted in rooting medium. In case of Gymnema sylvestre, stem cuttings of length 15 cm, thickness 4-5 mm, having 4-5 bud should be dipped up to 5 cm in 750 ppm IBA for two minutes and transplanted in rooting medium.
  • ThesisItemOpen Access
    SURVEY AND DOCUMENTATION OF ETHNOMEDICINAL PLANTS IN TWO BLOCKS (ANGARA AND NAMKUM) OF RANCHI DISTRICT.
    (Birsa Agricultural University, Ranchi, Jharkhand-6, 2017) Suman, Kanchan Kumar; Abbas, S.G.
    Study of ethnomedicinal plants is the traditional management of human ailments. The study covered systematically selected of two blocks in Ranchi district. Within the selected two blocks, personal contacts were done. With thorough discussion with the help of Discussion, interviews and through field observations were done to collect data, and to take the a local contact person. Collected data was entered into Excel spreadsheet and summarized. The informations regarding Indegenous Technical Knowledge (I. T. K) were entered in a prescribed proforma which is mandatory under the IPR norms, all such proforma with their due signature are annexed in the thesis. All the data are summarized plant wise, plant parts and their uses in diseases. Under enumeration, the recent botanical names, family and vernacular names , habit, plant parts used in diseases, method of herbal formulation as used by traditional healers, are documented. Asparagus racemosus (Sataver), Mimosa pudica (Lajwanti), Madhuca indica (Mahua), Milletta pinnata (Karanj), Zingiber officinale (Adharakh), Heritiera fomes (Sundhari plant), Cajanus cajan (Arhar), Justicia adhatoda (Basak), Nyctantus arbortristis (Harsingar), Opuntia dillenii (Nagphani), Butea monosperma (Plash) were given. The people of the study areas and surroundings areas visit the traditional healers for various health problems, and the local healer treat their ailments by preparing medicine (formulations) from medicinal plants present in any around of their habitat and they charge very nominal fee. During field visit, it was observed that some healers hesitate to reveal the formulation or even not ready to divulge the name of particular plants and wants some many for sharing their I.T.K. Their reservation is also due to that if outside people will know this, they will eradicate them for their economical gain, and the plant will lose its effectiveness. They transfer the knowledge only to the main juni or family members, who will continue this practice after him. They dout want to transfer their knowledge to other seet or east or other veligion and consider this knowledge as gift of their family datei kuldevta. Transfer of knowledge among the community in the study areas has mainly been taking place from the father to the trusted eldest son or selected well behaved son through long time exposure and experience learned and gained from his father. Yet this knowledge could not be transferred freely outside blood relationship of traditional healers.
  • ThesisItemOpen Access
    EVALUATION OF WILD AND CULTIVATED GERMPLASM OF Withania somnifera (L.) DUNAL (ASHWAGANDHA)
    (Birsa Agricultural University, Kanke, Ranchi, Jharkhand, 2013) LAL, MOTI; Abbas, S.G.
    The present experiment entitled “Evaluation of Wild and Cultivated germplasm of Withania somnifera (L.) Dunal (Ashwagandha)” was conducted during the year 2011 at nursery site of the Faculty of Forestry, Birsa Agricultural University, Kanke, Ranchi to carry out systematic research trial on wild and cultivated trial of the species with the objectives a) To evaluate the comparative growth pattern of wild and cultivated germ plasm of Ashwagandha (Withania somnifera). b) To evaluate comparative yield potential of wild and cultivated germplasm of Ashwagandha (Withania somnifera) and c) To evaluate the comparative alkaloid content of wild and cultivated germplasm of Ashwagandha (Withania somnifera). In the present study, the wild and cultivated germplasm of Withania somnifera (L.) Dunal (Ashwagandha) are taken for experiment. The wild germplasm collected from Khunti forest area and the cultivated germplasm are JA-20, JA–134, Poshita. The experiment was conducted in Randomized Block Design (RBD) to know the growth and yield parameters of different germplasm of the Ashwagandha. The experiment was replicated five (5) times. The total numbers of treatments were four (4). The total area of the plot was 4m X 3m and number of plant in each plot was 100. Number of plants to be used for collection of data from each plot was ten (randomly). The growth parameters of Ashwagandha like Plant height (cm), Number of main branches, Number of berries per plant, Leaf length (cm), Days to flower initiation (days), Days to seed harvest (days), and the yield parameters like Number of seeds per berry, Seed yield per plant (gm) are recorded as per NBPGR format. The yield parameters of Ashwagandha like Main root length (cm), Main root diameter (cm), Number of secondary roots per plant, Fresh root weight per plant (gm), Dry root weight per plant (gm) were taken on final harvesting. The alkaloid content (%) and Fiber content (%) in root are estimated through HPLC method in the laboratory. Average value of pH, organic carbon, available nitrogen, phosphorus, and potassium of the soil of research area was estimated before plantation of Ashwagandha. The recorded value of different chemical parameters of soil are as follows pH (6.80), organic carbon (0.78), available nitrogen (345.25 kg/ha), available phosphorus (120.50 kg/ha) and available potash (252.60 kg/ha). The soil of the sites is sandy loam having water holding capacity (%) as 40.50 and moisture content (%) as 10.82. Maximum plant height among the germplasm was found in Poshita i.e. 98.00 cm whereas minimum height obtained in wild variety i.e. 68.4 0cm. Number of primary branches at full bloom stage varied from 4.00 to 5.00. Increasing order of the number of main branches is as follows: T1