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Birsa Agricultural University, Ranchi

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20132
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Subject: Animal Genetics and Breeding × End date: 2019 × Start date: 2013 × Type: Thesis × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    GENETIC ARCHITECTURE OF BLACK BENGAL GOAT IN CONTEXT TO ITS FECUNDITY
    (Birsa Agricultural University, Kanke, Ranchi, Jharkhand, 2013) KUMAR, VIVEK; Singh, L. B.
    The present study was carried out on Black Bengal goats which is prolific meat purpose breed of India. Animals were maintained at Instructional Small Ruminant Farm, Ranchi Veterinary College, B.A.U., Ranchi. In this investigation, goat population was screened for polymorphism of FecB gene. A study of association of different variants with different reproductive traits and absolute body weights at different stages of growth was also done. With the primers having forward and reverse base sequence as 5'-CCAGAGGACAATAGCAAAGCAAA-3' and 5'-CAAGATGTTTTC ATGCCTCATCAACAGGTC-3', PCR products obtained were subjected to polyacrylamide gel electrophoresis for the detection of Single Strand Conformational Polymorphism (SSCP). As a result, three different SSCP variants were found which were designated as AA, AB and BB. The highest genotype frequency was observed for AB (0.38), which was followed by BB (0.33) and AA (0.29). Least-square analysis of variance showed significant (P<0.01) effect of genotype on litter size at birth. The result revealed that highest litter size at birth was found to be 02.06±00.08 for genotype BB, which differed significantly (P<0.01) from that of genotype AA (01.14±00.08) and genotype AB (01.27±00.07). Further, it was found that genotype had significant (P<0.01) effect on litter size at weaning. The highest litter size at weaning was found to be 01.81±00.06 for genotype BB, which differed significantly from that of genotype AA (01.00±00.08) and genotype AB (01.12±00.06). There was non-significant effect of genotype on age at first kidding, service period, kidding interval and gestation period. Moreover, there is significant (P<0.01) effect of genotype on body weight at birth. The least square mean of body weights at birth for genotype BB (01.17±00.03 kg) was significantly lower than that of genotype AA (01.43±00.03 kg) and genotype AB (01.36±00.02 kg). The different genotype were non-significantly associated with absolute body weight at 4-week, 8-week, 12-week, 24-week, 36-week and 48-week. Nucleotide sequences of the allelic variants were also analyzed. The DNA sequence showing polymorphism observed were used to identify SNPs. Principal SNP was found at 78 position of gene sequence, which shows transition of Adenine (AA genotype) to Guanine (BB genotype). On the basis of this investigation, following conclusion were observed: 1. The PCR-SSCP analysis of FecB gene revealed the polymorphic pattern of genotypes in Black Bengal Goat. It suggests that flow of FecB mutation was present in Black Bengal Goat. 2. There was significant association of different polymorphic variants of FecB gene with the litter size at birth and at weaning and body weight at birth.
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    ThesisItemOpen Access
    GENETIC ARCHITECTURE OF CHHOTANAGPURI SHEEP IN CONTEXT TO ITS FECUNDITY
    (Birsa Agricultural University, Kanke, Ranchi, Jharkhand, 2013) ORAON, THANESH; Singh “Dron”, D. K.
    The present study was conducted to study polymorphism of FecB genes and ascertain genotypes of Chhotanagpuri lambs on the basis of alleles of FecB gene with objectives to estimate the effects of genotypes and some non- genetic factors on growth in terms of absolute body weights of lambs from birth to 52-week of age as well as variation in growth rate in terms of average daily gain in weight during different periods of growth. Attempts were also made to study relationship of ewe’s weight at lambing with growth of their lambs as well as among body weights at different stages of growth from birth to 52-week of age. Regression equations with their coefficient of determination were also developed to predict body weight at later stages of life on the basis of earlier weights. The findings of this study are being summarized as follows: (1) Six single strand conformation polymorphism variants of FecB gene were obtained at Locus-1 and Locus-2. (2) AT Locus-1, the genotype frequency of AA was highest (0.424) followed by AB (0.326) and BB (0.250) .revealed that the frequency of A and B alleles were 0.587 and 0.413, respectively. (3) Locus-2, the genotype frequency of CD was higher (0.402) followed by CC (0.381) and DD (0.217), indicating that the frequency C and D were 0.587 and 0.418, respectively. (4) Genotypes at Locus-1 had significant effect on body weights of lambs from 8th week of age to 52nd week of age, though the variations in birth weight and weight at 4th week due to it was not significant. (5) Lambs with BB genotype at Locus-1 had better growth rate up to 52-wk of age than those with AA and AB genotypes. (6) Growth of lambs remains unchanged due to genotypes at Locus 2, though lambs of CC genotype had better growth than CD and DD genotypes. (7) Body weights of lambs from 4th week onwards varied significantly due to dam’s weight at lambing. (8) Lambs of heavier dams continued to weigh more than those of lighter dams till 52- wk of age. (9) Birth weight of lambs had significant effect on their growth up to 52-week of age. Lambs born with higher birth weight at grow faster rate than those born having lower birth weight particularly during pre-weaning period i.e up to 12-week of age. (10) Average Daily Gain (ADG) in body weight of lambs was maximum (81.64±3.83 g/day) irrespective of genotypes. (11) ADG declined with the advancement in age. (12) Lambs with BB genotype at Locus had better growth rate in terms of ADG than those of AA and AB at this locus (13) Lambs with higher birth weight had better ADG particularly during pre-weaning period. (14) Dam’s weight at lambing had positive and significant phenotypic association with body weight of their lambs up to 52 – week of age. (15) Phenotypic association among body weights at various stages of growth were positive and significant, though the magnitude of relationship declined with the increase in gap between two weights taken. Regression study revealed that the body weight at growth could be predicted with moderately higher accuracy on the basis of preceding weight The following conclusions have been drawn: (1) Chhotanagpuri lambs with BB genotypes should be selected for better growth. (2) Breeding program should be designed to increase the frequency of ‘B’ allele among individuals in a flock of Chhotanagpuri sheep. (3) Lambs with high birth weight should be cared properly particularly after weaning at 12-wk of age for better post-weaning growth. (4) Keeping in view the positive association of dam’s weight at lambing on growth of their lambs up to 52-week of age the ewes particularly during advance stage of pregnancy should be fed properly and cared properly for improving birth weight of lambs and its growth. (5) Lambs could be selected on the basis of weight at at birth and 12-wk of age for better growth and more meat yield. (6) Polymorphism of FecB gene should be studied with a large number of primers to derive valid conclusion in context of fecundity.