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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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2016 - 201912020 - 20211
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Author: SHARMA, PUSHPINDER × Start date: 2016 ×
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    ThesisItemOpen Access
    ISOLATION AND SCREENING OF INDUSTRIALLY IMPORTANT MICROBES FOR THE PRODUCTION OF BIOMOLECULES WITH AN EMPHASIS ON BIOSURFACTANTS
    (UHF,NAUNI, 2021-01) SHARMA, PUSHPINDER; SHARMA, NIVEDITA
    ABSTRACT Biosurfactant research as well as its development and commercialization have beheld a great significance in recent years. Biosurfactants are considered as amphiphilic surface-active compounds produced by various microbes that harbor numerous unique properties. Oil contaminated sites are highly probable source of oil degrading microorganisms and was utilized as a source for isolation of biosurfactant producing microorganisms. In present study, 40 strains were isolated from biosurfactant producing bacteria from various oil-contaminated sites of Himachal Pradesh. Among them, four strains PS9, PS23, PS26 and PS37 were selected for the further study which were identified as B. tequelensis PS9 |MN 197768|, B. cereus PS23 |197769|, B. subtilis PS26 |197770| and Escherichia fergusoni PS37 |1997771|. Antibacterial and antifungal activity of the strain was also examined. Optimization of culture conditions was done on the basis of various process parameters and medium components viz. media types, temperature, pH, carbon sources, nitrogen sources and media additives firstly using classical One Variable at a Time (OVAT) approach followed by statistical optimization by utilizing Central Composite Design (CCD) of Response Surface Methodology (RSM). Hydrophobicity was found to be maximum at 40°C temp in 1% carbon and nitrogen source in both the strains but at 7.0 and 9.0 pH in B. tequelensis and B. subtilis respectively. Furthermore, 283mg of dried biosurfactant was produced. The extraction and purification was done followed by quantitification of biosurfactant formed by B. tequelensis PS9 which was done by using High Performance Liquid Chromatography (HPLC). The quantity of the biosurfactant came out to be 85.657ppm. Further characterization of purified biosurfactant was done and it was found to be effective at 40°C temperature, 7.0 pH, 1% NaCl concentration and quite stable at 4°C for 9 days while at room temperature upto 5 days. The maximum of 98.25μg/ml protein content was observed. In oildisplacement activity, an impressive zone of 2.6cm was observed and emulsification index was equal to 37.5%. Molecular and structural compositions of the purified biosurfactant were then evaluated by FTIR, NMR and MS/MS and a cyclic lipopeptide {C14 Surfactin (L/I4, D6), [M+H]+ and C15 Surfactin (V4,D6), [M+H]+} by Bacillus tequelensis PS9 were identified. In case of other analytical techniques, FTIR data revealed lactone and peptide form of surfactin, NMR showed glycosidic linkage of sugar moiety and methoxy functional groups indicating the surfactin. The study has a great implication as the biosurfactants are considered safer alternative to synthetic or chemical surfactants as they are less toxic, eco-friendly and of course have low ecological impact. The present study provides further evidence that biosurfactant produced from Bacillus tequelensis PS9 has undoubtedly turned out to be a potential candidate in bioremediation as well as can be recommended for industrial use as a laundry detergent
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    ThesisItemOpen Access
    EXPLORATION OF MICROBIAL DIVERSITY OF HIMACHAL PRADESH FOR PRODUCTION AND OPTIMIZATION OF LIPASE AND ITS APPLICATION
    (UHF,NAUNI, 2016) SHARMA, PUSHPINDER; SHARMA, NIVEDITA
    ABSTRACT Enzymes are considered as nature’s catalysts. Lipases are a class of enzymes which catalyze the hydrolysis of long chain triglycerides into polar lipids. Oil contaminated sites are highly probable source of lipogenic microorganisms and was utilized as a source for isolation of lipase producing microorganisms. In total, 50 lipoltytic bacteria have been screened from oil contaminated sites of Himachal Pradesh. Among them hyper lipolytic strains PS3 and PS4 were selected and identified as Bacillus methylotrophicus PS3 KX758632 and Brevibacterium halotolerans PS4 KX671556. Cultural conditions and process parameters viz. media types, pH, temperature, inoculum size, incubation time, substrate concentration, divalent ions and surfactants etc. were optimized firstly through classical one variable at a time (OVAT) followed by statistical optimization by employing central composite design of response surface methodology. The enzymes obtained from both the strains were purified to homogeneity by following a sequential purification approach. B. methylotrophicus PS3 lipase was purified to a final purification fold of 2.90 and had a molecular weight of  31.5 kDa whereas B. halotolerans PS4 lipase was purified to a final purification fold of 2.94 and had a molecular weight of  80 kDa. Lipase activity was found to be maximum at 40oC and pH 7.0 for both the strains. Applicability of purified lipase from both the strains was assessed by utilizing it for the removal of stains from the clothes. Both these strains along with branded detergents had significantly improve the cleansing of grease, butter, vegetable oil, olive oil and grease (white) within 30min as compared to the control. The high efficiency of lipase in enhancing the washing quality of detergents by destaining makes them a potential candidate for use in detergent industry.