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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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20121
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Subject: Plant Pathology × Author: ANIL, KUMAR × End date: 2019 × Start date: 2010 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    CHARACTERIZATION OF A VIRUS CAUSING MOSAIC DISEASE IN CUCUMBER (Cucumis sativus L.)
    (2012) ANIL, KUMAR; THAKUR, P.D.
    ABSTRACT Surveys during 2011-12 revealed the incidence of mosaic disease in cucumber (Cucumis sativus L.) varying from 0-66 per cent in Solan and Sirmour districts of Himachal Pradesh. The characteristic symptoms exhibited by present isolate both under natural and glass house conditions were vein clearing, vein banding, mild mosaic to yellow mosaic, blistering on leaves along with stunted growth of vines bearing small sized distorted fruits. Three isolates representing distant locations were found to be similar as far as their reaction on certain indicator hosts (Nicotiana glutinosa L., N. tabacum var. White Burley, N. debneyii L., N. occidentalis “37B”, Chenopodium album L., C. amaranticolor Coste and Reyn, C. quinoa Wild, Datura metel L., and D. metel var. festuasa) and serological identity with potyvirus group specific antibodies is concerned. An isolate was then further selected for partial characterization. The isolate was easily transmissible through sap as well as by aphid vectors Myzus persicae and Aphis craccivora and it was not seed and soil transmissible. The host range studies revealed that the virus isolate could infect Amaranthus caudatus L., Capsicum annuum L. (chilli), Capsicum annuum L. var. “California Wonder”, Chenopodium album L., Chenopodium amaranticolor Coaste and Reyn., C. quinoa Wild, Callistephus chinensis L. var. Sushank, Citrullus lanatus (Thunb), Cucurbita pepo L. var. Australian Dark Green, Cururbita pepo L var. Pusa Alar, C. moschata (Duch.) Poir,Cucumis melo L. var. “Hara Madhu” Cucumis sativus L., Gomphrena globosa (L.), Datura metel L., Datura metel L. var. festuosa, Lagenaria siceraria (Molina) Standl., Luffa acutangula (L.) Roxb., Luffa cylindrica Roem., Momordica charantia L., Nicotiana glutinosa L., N. tabacum L. var. “White Burley”, N. debneyii Dowin., Nicotiana occidentalis “37B”, Phaseolus vulgaris L. var. “Jwala”, Phaseolus vulgaris L.var. Pinto, Physalis floridana Rydb., Pisum sativum L., Petunia hybrida Vilm, Raphanus sativus L., Vicia faba L., Vigna sinensis Savi, Zinnia elegans Jacq, species. However, it was not found to infect Abelmoschus esculentus Monech, C. murale L., Lycopersicon esculentum Mill var. “Solan Gola”, Solanum nigrum L., Solanum tuberosum L., Solanum melongena L. and Tagetes erecta L. var. Pusa Narangi plant species. Biophysical properties of the virus isolate included TIP of 65-70°C, DEP of 10-3-10-4 and LIV of 4 days at room temperature (22-26°C) and 8 days under refrigeration (4+1oC). Serological identification of virus isolate through DAC and DAS-ELISA showed strong positive serological relationship with WMV-2 antiserum besides a mild reaction with ZYMV antibodies. Morphologically, virus isolate had flexuous rod shaped particles and ultrathin section studies revealed the presence of scroll inclusions and virus particles in cytoplasm. The present virus isolate was also successfully detected in RT-PCR assay by using potyvirus group specific primers (CPUP & P9502) and resulted into a desired product of ~725 bp. Hence, based on the results of identification parameters particularly host range, transmission, particle morphology, serological relationship and RT-PCR detection, the present virus isolate had been identified to be similar to WMV-2 belonging to potyvirus genus in family potyviridae. Further studies on molecular characterization will confirm its exact identity and homology with other WMV-2 reported from different parts of the world. In screening of eighteen varieties/hybrids and twenty five local collections of cucumber under field conditions revealed that eight local collections of cucumber were immune to the present virus isolate as no symptoms were observed on these collections.