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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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20201
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Subject: Biotechnology and molecular Biology × Author: Devi, Bandna × Start date: 2010 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    ASSESSMENT OF GENETIC FIDELITY OF IN VITRO RAISED PLANTS OF GLORIOSA SUPERBA L. THROUGH MOLECULAR MARKERS
    (COLLEGE OF HORTICULTURE AND FORESTRY, DR Y S P UHF, NERI, HAMIRPUR, 2020-06-22) Devi, Bandna; Sharma, Sneh
    The present investigation on the “Assessment of genetic fidelity of in vitro raised plants of Gloriosa superba L. through molecular markers” was carried out in the Department of Biotechnology. Gloriosa superba L. is an important endangered medicinal plant belongs to family Colchicaceae. It is a perennial tuberous herb extensively scattered in tropical and sub-tropical parts of India. Due to the medicinal value, these plants are collected from wild as raw material for large scale medicinal industry, leading to overexploitation and it is the main reason to include this plant species in the Red Data Book. Hence, to overcome this problem micropropagation is an alternate and effective method. An efficient in vitro regeneration protocol has been established using shoot tips and tuber explants. Explants were sterilized using different concentrations (0.05-1.0 w/v) of mercuric chloride for varying time exposure. Explants were cultured on MS medium containing different concentrations and combinations of cytokines (BAP, NAA and Kn). The best shoot induction was observed on MS medium supplemented with 2.0 mg/l BAP in case of tuber explants and in case of shoot tips explants best shoot induction was observed on MS medium supplemented with 1.5mg/l BAP and 0.5 mg/l NAA. Best rooting was obtained from shoots cultured on half-strength of MS medium fortified with 2.0 mg/l IBA. In vitro raised shoots were transferred to hardening media containing different combinations of substrates. Among all the combinations, sand: soil: vermicompost (1: 2: 1) produced the highest survival rate of 73.33%. A large number of plants can be produced in vitro under aseptic conditions, but there is always a danger of producing somaclonal variants by tissue culture technology. Thus, the genetic fidelity of micropropagated clones was evaluated by using random amplified polymorphic DNA (RAPD) and inter specific sequence repeat (ISSR) analysis. During the study a total of 17 primers were screened, out of which 2 RAPD and 2 ISSR primers produced clear, distinct and reproducible amplicons. Fragments were scored for the presence of band as 1 (band present) and absence of band as 0 (band absent) and thus a matrix was obtained and further analyzed with NTSYS-pc software by using Jaccard’s coefficient to calculate the similarity matrix for mother and in vitro raised plants. Dendrogram was plotted using UPGMA between mother plant and in vitro plants of both, RAPD and ISSR, markers. A preliminary phytochemical analysis among the mother and in vitro raised plants showed a higher yield of secondary metabolites. The in vitro regeneration protocol provides a basis for germplasm conservation and also harnesses the various secondary metabolites of medicinal importance