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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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201018
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Subject: Biotechnology × End date: 2010 × Start date: 2010 × Type: Thesis ×
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Now showing 1 - 9 of 18
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    ThesisItemOpen Access
    Identification Of Quantitative Trait Loci For Resistance To Xanthomonas Campestris Pv. Campestris In Brassica Oleracea Var. Capitata
    (Dr Yashwant Singh Parmar University of Horticulture and Forestry;Solan, 2010) Saxena, Bhawna; Kaur, Rajinder
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    ThesisItemOpen Access
    Studies On Agrobacterium-Mediated Insect Resistance Gene Transfer In Tomato (Lycopersicon Esculentum Mill)
    (Dr Yashwant Singh Parmar University of Horticulture and Forestry;Solan, 2010) Sharma, Chhaya; Srivastava, D K
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    ThesisItemOpen Access
    “Studies on@-amylase inhibitor (@-AI) in some grain legumes”
    (UHF,NAUNI,SOLAN, 2010) TAANK, RICHA; NATH, AMARJIT K.
    ABSTRACT Eighteen different grain legume cultivars of Himalayan region were analyzed for -amylase inhibitor activity. -Amylase unit inhibited per gram seed weight was maximum in Hans cultivar of Phaseolus vulgaris L. -Amylase inhibitor from seeds of Hans cultivar of Phaseolus vulgaris L. was purified using ammonium sulphate precipitation, gel filtration chromatography (Sephadex G-200) and ion exchange chromatography (DEAE-Sephadex). The inhibitor was purified to homogeneity as judged by native PAGE with 34.42 fold purification and 69.35 per cent recovery. The purified inhibitor had a molecular weight of 16,218 daltons and was found to be monomer as revealed by SDS-PAGE. It was found to be heat stable upto 300C-400C and had two pH optima of 5.0 and 6.9. Nature of inhibition was found to be of non competitive type as determined by Lineweaver burk plot. The purified inhibitor was found to have inhibitory activity against -amylases extracted from larvae of Callosobruchus chinensis, Tribolium castaneum and mid gut of Corcyra cephalonica. Purified -amylase inhibitor was also found to inhibit human salivary - amylase.
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    ThesisItemOpen Access
    Effect of antibiotics on shoot regeneration and suppression of Agrobacterium growth in apple rootstock
    (UHF,NAUNI,SOLAN, 2010) CHAUHAN, ARJUN; MODGIL, MANJU
    Among 24 combinations of BA/TDZ and NAA/IAA, 1 mg/l of IAA and 4.0 mg/l of BA proved better and resulted in direct shoot regeneration (22.13%) of leaf explants. Antibiotics viz. cefotaxime, carbenicillin, timentin, augmentin and rifampicin were used to see their effect on callus/shoot regeneration, growth of bacteria in suspension cultures & for suppressing Agrobacterium tumefaciens growth during genetic transformation studies in apple rootstock MM111 using strain LBA4404 harboring the chitinase gene of rice within the vector pCAMBIA chi 11 with hygromycin phosphotransferase (hpt) as a selectable marker. All the antibiotics increased the callus induction with the increase in their concentration upto certain extent and declined at higher concentration. However, browning of leaf explants increased with the increase in concentration. Maximum callusing (85 – 93%) was observed in 300 mg/l Carb., 200 mg/l Aug. & 100-200 mg/l Tim., but no browning of leaf explants occurred only on Tim. Cefo. & Rif. showed reduced callus formation. It was also found that cfu/ml and OD values at 540 nm showed decrease with the increase in concentration of antibiotics added in bacterial suspension culture. Among all the antibiotics, Tim. & Aug. comparatively showed rapid decrease in OD values as the concentration increased of. No colony was observed in Tim. at 200 – 500 mg/l and Carb. & Aug. at 500 mg/l. Tim.(200 mg/l) and Aug.(300 mg/l) completely controlled the bacteria after co-cultivation even after 1st blot. At 500 mg/l, Cefo. showed the Agrobacterium growth in 25.07% of explants, whereas Carb. and Rif. showed no growth after 1st blot. But these antibiotics showed deleterious effects on the leaf explants at this concentration. Therefore, timentin(100-200 mg/l) and augmentin(300 mg/l) can be recommended for suppression of Agrobacterium growth during transformation experiments in apple rootstock MM111.
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    ThesisItemOpen Access
    STUDIES ON DATAMINING FOR DEVELOPMENT OF dbEST – SSRs FOR GENOTYPE IDENTIFICATION IN CAULIFLOWER (Brassica oleracea var. botrytis)
    (UHF,NAUNI,SOLAN, 2010) VAIDYA, ERA; KAUR, RAJINDER
    ABSTRACT The present investigation on cauliflower were carried out to develop EST – SSR markers from the EST database and to characterize different genotypes using genomic and dbEST – SSRs. EST sequences belonging to Brassica oleracea var. botrytis, Brassica oleracea var. capitata, Brassica oleracea var. italica, Brassica oleracea var. gemmifera and Arabdopsis thaliana, available on the EST database on the NCBI website (www.ncbi.nlm.nih.gov/nucest) were screened for SSR motifs using two programs i.e. MISA and SSRIT. 29 cauliflower, 165 Arabidopsis and 194 broccoli EST sequences were detected to contain SSRs and out of these, 16 sequences (two of cauliflower, 10 of broccoli and four of Arabidopsis thaliana) were selected for primer designing using PRIMER3 software. For polymorphism studies using 18 genomic SSR and 16dbEST – SSR primers, DNA from fresh, young leaves of 20 genotypes of cauliflower was isolated by CTAB method (Doyle and Doyle, 1987). Out of the 18 genomic SSRs, 16 gave amplification, eight of which were found to be polymorphic, revealing 65.85% polymorphism among the cauliflower genotypes. On the other hand, 13 out of 16 dbEST – SSRs amplified the genomic DNA, 11 primers being polymorphic, revealing 52.35% polymorphism. PIC (Polymorphism Information Content) value for all the primers was calculated and was found to range from 0.09 – 0.80 for genomic SSRs and from 0.09 – 0.60 for dbEST – SSRs. Similarity matrices and dendrograms were generated using NTSys ver.2.02h and dissimilarity matrices and rooted trees were generated using DARwin5 ver.5.0.155, for polymorphism data for both the primer sets. The dendrograms and rooted trees generated using UPGMA and Neighbour Joining algorithms, respectively, generated for both the SSR marker sets, divided the cauliflower genotypes into two main clusters, while genotype ‘US Agri Seeds’ was singled out from rest of the genotypes. Seven unique markers were given by three genomic SSRs and a single unique marker by one EST – SSR. These unique makers could identify three cauliflower genotypes.
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    ThesisItemOpen Access
    STUDIES ON in vitro PROPAGATION AND CONSERVATION OF Viola pilosa Blume
    (UHF,NAUNI,SOLAN, 2010) SONI, MADHVI; KAUR, RAJINDER
    Abstract A protocol for in vitro propagation and conservation was developed for Viola pilosa, a valuable medicinal plant The sterilized explants (buds) cultured on MS medium supplemented with 1 mg/l BA, 1 mg/l TDZ and 0.50 mg/l GA3 gave best results for in vitro shoot bud establishment. Although the problem of shoot vitrification occurred on this medium but this was overcome by transferring the vitrified shoot on MS medium supplemented with 1 mg/l BA and 0.25 mg/l Kn. The same medium was found out to be the best medium for in vitro shoot multiplication. Also effect of different types of solidifying agents on the growth of in vitro multiplying shoots was studied and out of three different solidifying agents viz. agar agar, agarose and gelrite gellan gum, the gelrite gellan gum containing medium was found out to be the best medium for in vitro shoot multiplication. Callus was obtained as a by product from shoots on MS medium supplemented with 1 mg/l BA, 1 mg/l TDZ and 0.50 mg/l GA3 and this callus was further multiplied on medium supplemented with 1.5 mg/l NAA. The shoot regeneration was obtained from the callus on MS basal medium supplemented with 0.1% activated charcoal. 100% root induction from in vitro grown shoots was obtained on half strength MS medium supplemented with 1 mg/l IBA. In vitro conservation was successfully demonstrated by using two different approaches namely slow growth at low temperature and cryopreservation following vitrification. In slow growth at low temperature studies the in vitro shoots incubated at 10oC, though showed 100% retrieval, however, shoots became etiolated, while the shoots incubated at 4oC showed retrieval of 85.7% with no morphological variation during incubation. In cryopreservation - vitrification studies the vitrified shoot buds gave maximum retrieval of 41.66% when they were precooled at 4oC while only 16.66% vitrified shoots were retrieved from those precooled at 10oC. In vitro formed plantlets were hardened and transferred to soil with 55.55% survival.
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    ThesisItemOpen Access
    GENETIC IDENTIFICATION OF APPLE GENOTYPES BY USING MOLECULAR MARKERS
    (2010) PATHANIA, PARDEED; MODGIL, MANJU
    ABSTRACT Random amplified polymorphic DNA (RAPDs) and simple sequence repeats (SSR) were used to test the genetic variability and relatedness among 20 cultivars of apple. 28 RAPD and 5 SSR primers were used to amplify the DNAs of all 20 cultivars. RAPD generated 107 polymorphic bands with 65.6% polymorphism while SSR generated 15 polymorphic alleles with 71.4% polymorphism. The average PIC value was 0.70 for RAPD and 0.64 for SSR and the PIC value was the highest in OPA-16(RAPD) and IISRS-3; IISRS-18(SSR) primers. RAPD and SSR revealed different genetic similarities among 20 apple cultivars. The highest similarity was detected between ‘Fuji’ and ‘Fuji Rekaki’ (0.859) followed by ‘Thanedar early flowering’ and ‘Fuji’ by RAPD while between ‘Galaxy’ and ‘Jonadel’ (1.000) followed by ‘Neomi’ with ‘Arlet’, ‘Fuji’ and ‘Royal Gala’; ‘Fuji Rekaki’ with ‘Thanedar early flowering’ and ‘Reinette-Du-Canada’ by SSR. The dendrogram generated from RAPD data grouped ‘Fuji’ and ‘Fuji Rekaki’ in one cluster and ‘Jonadel’ and ‘Jonagold’ in other cluster, while dendrogram derived from SSR data grouped ‘Jonadel’ and ‘Jonagold’ in one cluster and ‘Arlet’ and ‘Royal Gala’ in other cluster, which is generally in accordance with the recorded pedigree information
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    ThesisItemOpen Access
    DEVELOPMENT OF MASS MULTIPLICATION PROTOCOL FOR ASPARAGUS ADSCENDENS ROXB.
    (2010) SHARMA, ANISH KUMAR; BHARDWAJ, S.V.
    ABSTRACT The present investigations entitled ‘‘Development of mass multiplication protocol for Asparagus adscendens Roxb.” were conducted to obtain plantlets through direct regeneration from juvenile nodal segments and rhizome explants from mature plants. Nodal segments obtained after excising spines and cladodes while tuberous roots from rhizome explants were carefully removed using scalpel blade without damaging the explants. Nodal segments were surface sterilized with 0.525% NaOCl for 15 minutes followed 0.2% bavistin for 15 minutes and finally 0.1% HgCl2 for 1.5 minutes and resulting in 91.33% survival. The rhizome explants were sterilized using 3% NaOCl for 2 minutes followed by 2% bavistin + 2% Mancozeb for 20 minutes and finally 0.1% HgCl2 for 2 minutes achieving 88.3% survival of explants. Maximum establishment of cultures from both explants was obtained on MS medium supplemented with 0.2 mg/l BAP and 0.2 mg/l Kn. In nodal explants maximum shoot proliferation rate (5.50) on MS medium containing 0.05 mg/l NAA and 0.3 mg/l Kn was obtained, while in rhizome explants multiplication rate of 3.50 was obtained on MS medium with 0.2 mg/l BAP and 0.2 mg/l Kn after 8 weeks on multiplication medium. Regenerated shoots of appropriate size (3- 4cm) were transferred to 14 different media combinations tried for root induction but failed to get any success which can attributed due to genetic variation in the concerned species. On the basis of this study it is concluded that a reliable protocol for in vitro shoot multiplication of Asparagus adscendens has been developed which can be used for conservation of this endangered plant species.
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    ThesisItemOpen Access
    In Vitro selection and regeneration of hybrid lily against the culture filtrate of Fusarium oxysporum f. sp. lilii
    (2010) SHARMA, Anshu; SHARMA, S.K.
    ABSTRACT The reproducible protocol was optimized for in vitro selection and regeneration of hybrid lily against culture filtrate of Fusarium oxysporum f.sp. lilii. Callus initiation was recorded on medium AS19 (MS basal medium +2 mg/l NAA +1.5 mg/l BA, 5% sucrose when incubated under dark condition. Calli were subjected to different concentrations of culture filtrate of Fusarium oxysporum f. sp. lilii where selective dose of culture filtrate was found to be 15 per cent on which 6.6 per cent cell survival of lilium cv. ‘Casa Blanca’ was recorded. The best medium for bulblet regeneration was ASR4 (MS basal medium + 2mg/l NAA+1.5 mg/l BA and 3% sucrose) on which 75.0 per cent calli regenerating bulblets were recorded. Per cent survival of the bulblets regenerating from the selected calli when tested in vitro with spore suspension was 10 per cent respectively.