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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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20221
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Subject: Biotechnology × Author: AMAN PREET SINGH × Start date: 2020 ×
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    ThesisItemOpen Access
    MORPHOLOGICAL, BIOCHEMICAL AND MOLECULAR EVALUATION OF TISSUE CULTURE RAISED CLONAL APPLE ROOTSTOCKS
    (UHF,NAUNI, 2022-11) AMAN PREET SINGH; THAKUR, MANISHA
    ABSTRACT Apple (Malus × domestica Borkh.) is an important cash crop and has been commercially grown in the temperate region of the world. For commercial apple production, seedling rootstocks have been used for propagation for a long time, but now clonal asexually propagated rootstocks have become popular because of their wider ecological range, early maturity, fruit quality, resistance to diseases and pests, and ability to adapt to different climates and soils. This makes them better than seedling rootstocks. It is also a cheaper and more effective way to make clonal rootstocks. To address certain issues related to grafting and performance of tissue culture raised apples the studies entitled “Morphological, biochemical and molecular evaluation of tissue culture raised clonal apple rootstocks” were conducted in an experimental field layout maintained by the Department of Fruit Science having different apple clonal rootstocks viz. M7, M9, MM106, MM111, and Merton 793 have been produced through in vitro propagation in the Department of Biotechnology, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh. The morphological parameters showed that there was no variation in leaf type and shape in apple trees raised on conventionally propagated and tissue culture raised clonal rootstocks. All the leaves were of Crenate, Crenate to Serrate, and Serrate type and V-shaped, concave, convex, flat with raised margins and flat shapes. Early flowering was observed in trees raised on tissue culture propagated rootstocks in comparison with conventionally propagated rootstocks. The maximum tree height (189.99cm) and inter-node length (4.6cm) were observed in trees raised conventionally propagated Merton 793 whereas, the maximum leaf area was observed in trees on tissue culture raised M9 (30.11cm2) rootstock. Maximum photosynthesis (13.18μmol/m²/s) was observed in trees raised on conventionally propagated MM111 rootstock whereas, maximum stomatal conductance (0.20μmol/m²/s) and transpiration (7.54μmol/m²/s) was recorded in trees raised on conventionally propagated Merton 793. The biochemical evaluation revealed maximum protein content (3.80mg/g) was recorded in trees raised on tissue culture raised Merton 793 rootstock. Trees raised on conventionally propagated MM111 rootstock depicted maximum total sugar (163.67mg/g), reducing sugar (116.91 mg/g), and non-reducing sugar (46.76mg/g). The maximum Total phenolic content (TPC) (327.74mg/g) in trees raised on tissue culture raised MM106 rootstock their molecular analysis using RAPD and SCoT primers to draw a similarity between tissue culture raised and conventionally propagated apple clonal rootstocks grafted with cv. Jeromine. Out of 33 RAPD primers, 18 primers were successful in DNA amplification resulting in 64 amplicons, out of which 58 were monomorphic. Nineteen out of 36 SCoT primers resulted in DNA amplification leading to 83 amplicons, out of which 36 were monomorphic and 47 were polymorphic. Maximum Polymorphic Information Content (PIC) value for RAPD and SCoT primers were found to be 0.207 and 0.225 respectively. Tissue culture raised and conventionally propagated apple rootstocks grafted with cv. Jeromine was significantly similar at morphological, biochemical, and molecular levels. Hence tissue culture raised clonal apple rootstocks can be recommended to the farmers for raising high density apple plantations for commercial gains