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Assam Agricultural University, Jorhat

Assam Agricultural University is the first institution of its kind in the whole of North-Eastern Region of India. The main goal of this institution is to produce globally competitive human resources in farm sectorand to carry out research in both conventional and frontier areas for production optimization as well as to disseminate the generated technologies as public good for benefitting the food growers/produces and traders involved in the sector while emphasizing on sustainability, equity and overall food security at household level. Genesis of AAU - The embryo of the agricultural research in the state of Assam was formed as early as 1897 with the establishment of the Upper Shillong Experimental Farm (now in Meghalaya) just after about a decade of creation of the agricultural department in 1882. However, the seeds of agricultural research in today’s Assam were sown in the dawn of the twentieth century with the establishment of two Rice Experimental Stations, one at Karimganj in Barak valley in 1913 and the other at Titabor in Brahmaputra valley in 1923. Subsequent to these research stations, a number of research stations were established to conduct research on important crops, more specifically, jute, pulses, oilseeds etc. The Assam Agricultural University was established on April 1, 1969 under The Assam Agricultural University Act, 1968’ with the mandate of imparting farm education, conduct research in agriculture and allied sciences and to effectively disseminate technologies so generated. Before establishment of the University, there were altogether 17 research schemes/projects in the state under the Department of Agriculture. By July 1973, all the research projects and 10 experimental farms were transferred by the Government of Assam to the AAU which already inherited the College of Agriculture and its farm at Barbheta, Jorhat and College of Veterinary Sciences at Khanapara, Guwahati. Subsequently, College of Community Science at Jorhat (1969), College of Fisheries at Raha (1988), Biswanath College of Agriculture at Biswanath Chariali (1988) and Lakhimpur College of Veterinary Science at Joyhing, North Lakhimpur (1988) were established. Presently, the University has three more colleges under its jurisdiction, viz., Sarat Chandra Singha College of Agriculture, Chapar, College of Horticulture, Nalbari & College of Sericulture, Titabar. Similarly, few more regional research stations at Shillongani, Diphu, Gossaigaon, Lakhimpur; and commodity research stations at Kahikuchi, Buralikson, Tinsukia, Kharua, Burnihat and Mandira were added to generate location and crop specific agricultural production packages.

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20231
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Subject: Agricultural Biotechnology × Author: BORDOLOI, SUBHROTA × End date: 2023 × Start date: 2023 × Type: Thesis ×
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    ThesisItemOpen Access
    MOLECULAR ANALYSIS FOR TRAITS RELATED TO DROUGHT ADAPTATION IN RICE (Oryza sativa L.)
    (2023) BORDOLOI, SUBHROTA; Baruah, Akhil Ranjan
    Drought is a major abiotic constraint for rice production worldwide. Model based studies revealed reproductive stage drought stress (RSDS) and intermittent drought stress (IMDS) are expected to reduce yield significantly in Sali and Ahu season, respectively. The present study was conducted with the aim to detect the genetic effects of water stress through quantitative trait locus (QTLs) associated with drought tolerance in rice. The donor and the recipient parent were selected based on the phenotypic evaluation and gene expression analysis using five drought responsive genes in two drought tolerant cultivar (ARC10372, Koimurali) and two susceptible cultivar (Disang, IR-64). Based on the results, Koimurali was crossed with a drought susceptible cultivar, Disang to obtained true F1 seeds. Subsequently the single F1 seeds was selfed for and advanced to subsequent generations (F5:6) using single seed descent (SSD) method to develop the mapping population consisting of 247 RILs. Parental polymorphism study was conducted by screening 600 SSR markers; among which 98 SSR markers were found to be polymorphic (16.33%). The RILs along with their parents were genotyped using the 98 polymorphic SSR markers and a linkage map was constructed covering a distance of 2495.31cM spanning across the 12 linkage groups using the software JoinMap 4.0. The phenotyping score of two different stress condition (reproductive stage drought stress stress and intermittent drought stress condition) were compared with the genotypic data for identification of QTLs. The QTL analysis detected eight QTLs at reproductive drought stress located on chromosome number 3(qGY3LOD 3.54, qYSI3LOD3.54); 4(qGY4LOD 2.57, qTOL4 LOD 2.56, qYSI4 LOD 2.56, qYSI4 LOD 2.59, qRYR4 LOD 2.58, qDSI4, LOD 2.62) and 11 QTLs at intermittent drought stress located on chromosome1 (qFLW1, LOD 2.23, qNOC1 LOD 2.7, qNOG1 LOD 1.83); 3(GLW3 LOD 2.55), 4(qGY4 LOD 2.7, qRYR4 LOD 2.35, qTOL4 LOD 3.5, qTOL4 LOD 2.2, qYSI4 LOD 2.35), 7(qNOG7 LOD 2.91, qTNG7 LOD 2.84). The detected QTL regions were scanned to identify putative genes associated with drought tolerance in rice in both the stress conditions. The putative gene in reproductive drought stress were used to check the expression in four extreme breeding line of different drought tolerant capacities. QTL comparision between both the stress conditions were evaluated and one QTL was found common and few shared the same chromosome but in different marker intervals indicating different genetics might control the underlying stress conditions. The putative QTLs and candidate genes identified in the present study need to be further validated, which will be helpful for the improvement of drought tolerance in rice.