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Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola

Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola was established on 20th October, 1969 with its head-quarter at Akola. This Agricultural University was named after the illustrious son of Vidarbha Dr. Panjabrao (alias Bhausaheb) Deshmukh, who was the Minister for Agriculture,Govt. of India. The jurisdiction of this university is spread over the eleven districts of Vidarbha. According to the University Act 1983 (of the Government of Maharashtra), the University is entrusted with the responsibility of agricultural education, research and extension education alongwith breeder and foundation seed programme. The University has its main campus at Akola. The instructional programmes at main campus are spread over in 5 Colleges namely, College of Agriculture, College of Agricultural Engineering & Technology, College of Forestry, College of Horticulture and Post Graduate Institute. At this campus 4 degree programmes namely B.Sc.(Agri.) B.Sc. (Hort.), B.Sc. (Forestry) and B.Tech. (Ag. Engg.) , two Master’s Degree Programmes viz. M.Sc.(Agri.) and M.Tech. (Agri.Engg.) and Doctoral Degree Programmes in the faculties of Agriculture and Agril. Engineering are offered. The University has its sub-campus at Nagpur with constituent College, College of Agriculture which offers B.Sc.(Agri.) and M.Sc.(Agri.) degree programmes. The Nagpur Campus is accomplished with a garden, surrounded by its natural beauty and a well established Zoo which attract the general public and visitors to the city. A separate botanic Garden is being maintained on 22 hectares with a green house for the benefit of research workers. In addition there are 2 affiliated grant-in-aid colleges and 14 private non-grant-in-aid colleges under the umbrella of this University A Central Research Station is situated at the main Campus which caters to the need of research projects undertaken by Crop Scientists of the principle crops of the region are Cotton, Sorghum, Oilseeds and Pulses.

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20112
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Subject: Horticulture × Author: PAITHANKAR, DINESH HARIDAS × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    GENETIC DIVERSITY STUDIES IN BRINJAL (Solanum spp.) USING MORPHOLOGICAL, BIO-CHEMICAL AND MOLECULAR MARKERS
    (Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2011-04-29) PAITHANKAR, DINESH HARIDAS; Nagre, Dr. P. K
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    ThesisItemOpen Access
    GENETIC DIVERSITY STUDIES IN BRINJAL (SOLANUM SPP.) USING MORPHOLOGICAL, BIO-CHEMICAL AND MOLECULAR MARKERS,
    (Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2011-04-29) PAITHANKAR, DINESH HARIDAS; Nagre, Dr. P. K
    The present investigation enti tled "Genetic diversity studies in brinjal (Solanum spp.) using morphological, bio-chemical and molecular markers" was undertaken with the objectives to estimate the diversity among 26 genotypes of brinjal (Solamun spp.) using morphological. biochemical and molecular markers and find the suita bil ity of different marker systems to study the genetic diversity in brinjal for their uti liza tion in hybridization programme. xii The analysis of variance revealed significant differences among the genotypes for all the characters under tudy. Canonical analysis indicated about 92.44 per cent of total variation was accounted by the four canonical roots. Fruits per cluster (vector 1), plant height (vector II), days to 1" harvest (vector Il l) and fruit length (vector IV) were important sources of variation on respective vectors and also confirmed by cluster mean for different characters. Seed protein profi le was studied by SDS-PAGE and esterase as well as peroxidase profile using Native PAGE technique. RAPD technique was used to assess genetic diversity by means of molecular markers. A total 173 polymorphic amplified products were obtained from 20 decamer primers which discriminated all the accession. All 26 genotypes were grouped on the basis morphological markers (three clusters), protein markers (seven clusters), esterase markers (four clusters), peroxidase markers (three clusters) and RAPD markers (nine clusters). Clustering done by morphological marker were not in agreement with biochemical and molecular markers. Diagnostic RAPD's were identified which discriminate among the Solanum species. Two brinjal accessions misclassified based on morphological traits were reassigned individual cluster using RAPD's. Similarly, protein markers next to the molecular marker could class ify Solanum species to the different cluster precisely. Different cross combinations were suggested on the basis of protein markers (81) esterase markers (146) and RAPD markers (133).However, the common cross combinations in protein , esterase and RAPD markers reduced the number of cross combinations to twenty five to expect relatively good amount of heterosis in F, and to get high frequency of transgressive segregants in subsequent generation which would be further utilized in breeding programme.Using RAPD marker, a total 15 unique bands were observed in a set of brinjal genotypes which could be uti lized for the identification of genotypes. RAPD primers OPA-04, OPA-05, OPA-16, OPB 10, OPB 12, OPH 02 , OPH 04 and OPH 05 shown highest polymorphism in a set of brinjal genotypes, hence these primers were most useful for the discrimination of brinjal genotypes. The present study indicates usefulness of assessing genetic diversity at DNA level using RAPD markers and its potential to determine the similarity between genotypes more precisely as compared to biochemical and morphological markers.