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Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola

Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola was established on 20th October, 1969 with its head-quarter at Akola. This Agricultural University was named after the illustrious son of Vidarbha Dr. Panjabrao (alias Bhausaheb) Deshmukh, who was the Minister for Agriculture,Govt. of India. The jurisdiction of this university is spread over the eleven districts of Vidarbha. According to the University Act 1983 (of the Government of Maharashtra), the University is entrusted with the responsibility of agricultural education, research and extension education alongwith breeder and foundation seed programme. The University has its main campus at Akola. The instructional programmes at main campus are spread over in 5 Colleges namely, College of Agriculture, College of Agricultural Engineering & Technology, College of Forestry, College of Horticulture and Post Graduate Institute. At this campus 4 degree programmes namely B.Sc.(Agri.) B.Sc. (Hort.), B.Sc. (Forestry) and B.Tech. (Ag. Engg.) , two Master’s Degree Programmes viz. M.Sc.(Agri.) and M.Tech. (Agri.Engg.) and Doctoral Degree Programmes in the faculties of Agriculture and Agril. Engineering are offered. The University has its sub-campus at Nagpur with constituent College, College of Agriculture which offers B.Sc.(Agri.) and M.Sc.(Agri.) degree programmes. The Nagpur Campus is accomplished with a garden, surrounded by its natural beauty and a well established Zoo which attract the general public and visitors to the city. A separate botanic Garden is being maintained on 22 hectares with a green house for the benefit of research workers. In addition there are 2 affiliated grant-in-aid colleges and 14 private non-grant-in-aid colleges under the umbrella of this University A Central Research Station is situated at the main Campus which caters to the need of research projects undertaken by Crop Scientists of the principle crops of the region are Cotton, Sorghum, Oilseeds and Pulses.

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20171
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Subject: Agricultural Biotechnology × Author: KAD, MISS. SNEHAL KRISHNA × End date: 2018 × Start date: 2017 ×
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    ThesisItemOpen Access
    ANALYSIS OF DNA METHYLATION PATTERN ASSOCIATED WITH FLORAL BUD DISTORTION IN SOYBEAN (Gylcine max L.).
    (Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2017-08-28) KAD, MISS. SNEHAL KRISHNA; Jadhav, Dr. P. V.
    The present investigation entitled, “Analysis of DNA methylation pattern associated with floral bud distortion in soybean (Glycine max L.)” was carried out during 2016-17 at Biotechnology Centre, Department of Agricultural Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola to investigate methylation pattern associated with floral bud distortion in soybean. DNA methylation is known to play an important role in regulating gene expression during biological development and tissue differentiation in eukaryotes. In the present attempt, cytosine methylation profile was determined in elite soybean (Glycine max L.) cultivar JS-335. Methylation-sensitive amplified polymorphism was used to generate genome-wide methylation profiles and compared the patterns of cytosine methylation in floral bud distorted and asymptomatic plant. It has been learned that soybean plant is affected by the disorder known as floral bud distortion (FBD), no pod formation take place at R6 stage which results in huge yield loss in symptomatic plant(s). The methylation-sensitive amplified polymorphism technique (MSAP) which is a modification of amplified fragment length polymorphism (AFLP) used to investigate the epigenetic changes associated with FBD in soybean. The samples of genomic DNA were double-digested with either HpaII or MspI and with EcoRI. To analyze epigenetic changes, 25 MSAP primer combinations detected a few DNA methylation patterns in the plant tissue of symptomatic and asymptomatic plant. A total of 378 and 392 clear and reproducible bands were amplified from symptomatic and asymptomatic samples, respectively. In the symptomatic plant, out of 378 MSAP sites, 273 (72.22%) were found un-methylated, 31 (8.20%) were hemi-methylated, 35 (9.25%) were fully methylated; and 39 (10.31%) hyper-methylated sites were amplified. Whereas, in asymptomatic plant, out of 392 MSAP sites, 315 (80.35%) found un-methylated, 21(5.35%) hemi-methylated, 29 (7.39%) fully methylated and 25 (60.37) hyper-methylated sites were amplified. An increased level of methylation was recorded in symptomatic plant (27.28%) than that of asymptomatic (19.13%). In fact, there was evidence of increase in fully methylated ratio to 19.57% in symptomatic plant and that in case of asymptomatic revealed decrease of 12.75%. These results indicated that there were greater differences of the genomic DNA methylation extent and also variation in the pattern of methylation between the symptomatic and asymptomatic plant. It revealed that the methylation status varied substantially during the development stage of flower. The polymorphic DNA methylation fragments that showed polymorphic bands were excised from Polyacrylamide gel and cloned in vector pTZ57R/T. However, total 10 polymorphic bands were excised varying from 150 to 250 bp, out of which only 2 bands were successfully purified from the PAGE. Further these methylated fragments were cloned in vector pTZ57R/T (2886 bp). These findings successfully generated extent and pattern of cytosine methylation in floral bud distorted and asymptomatic plants. In the present investigation, although limited numbers of methylation sensitive polymorphic fragments were obtained this helped to calculate the extent and pattern of methylation associated with floral bud distortion in soybean.