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Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola

Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola was established on 20th October, 1969 with its head-quarter at Akola. This Agricultural University was named after the illustrious son of Vidarbha Dr. Panjabrao (alias Bhausaheb) Deshmukh, who was the Minister for Agriculture,Govt. of India. The jurisdiction of this university is spread over the eleven districts of Vidarbha. According to the University Act 1983 (of the Government of Maharashtra), the University is entrusted with the responsibility of agricultural education, research and extension education alongwith breeder and foundation seed programme. The University has its main campus at Akola. The instructional programmes at main campus are spread over in 5 Colleges namely, College of Agriculture, College of Agricultural Engineering & Technology, College of Forestry, College of Horticulture and Post Graduate Institute. At this campus 4 degree programmes namely B.Sc.(Agri.) B.Sc. (Hort.), B.Sc. (Forestry) and B.Tech. (Ag. Engg.) , two Master’s Degree Programmes viz. M.Sc.(Agri.) and M.Tech. (Agri.Engg.) and Doctoral Degree Programmes in the faculties of Agriculture and Agril. Engineering are offered. The University has its sub-campus at Nagpur with constituent College, College of Agriculture which offers B.Sc.(Agri.) and M.Sc.(Agri.) degree programmes. The Nagpur Campus is accomplished with a garden, surrounded by its natural beauty and a well established Zoo which attract the general public and visitors to the city. A separate botanic Garden is being maintained on 22 hectares with a green house for the benefit of research workers. In addition there are 2 affiliated grant-in-aid colleges and 14 private non-grant-in-aid colleges under the umbrella of this University A Central Research Station is situated at the main Campus which caters to the need of research projects undertaken by Crop Scientists of the principle crops of the region are Cotton, Sorghum, Oilseeds and Pulses.

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20221
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Subject: Agricultural Biotechnology × Author: Authors : KHODE, VAIBHAVI. × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Title : ASSESSMENT OF SILICON EFFECT IN RESPONSE TO CHARCOAL ROT IN SOYBEAN (Glycine max L.).
    (Publisher : Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2022-10-04) Authors : KHODE, VAIBHAVI.; Advisor : JADHAV, Dr. P. V.
    Abstract : The present study entitled “Assessment of silicon effect in response to charcoal rot in soybean (Glycine max L.)” was carried out at Department of Agricultural Botany, Dr. PDKV, Akola during the academic year 2020-21 to evaluate the effect of silicon in response to charcoal rot disease in soybean genotypes. Considering the severity of the disease, potassium silicate (K2SiO3) which is known for activating defense mechanism and enhancing the morphological characters in plants. To identify effect of K2SiO3, mass culture of M. phaseolina was prepared for in-vitro screening under sick pot condition for charcoal rot tolerance in soybean genotypes. Silicon gets accumulated in higher concentration in the epidermal cells and has been detected to benefit the plants that are exposed to biotic stresses. The susceptible genotype, TAMS-38 in charcoal rot infected condition showed disease symptoms whereas resistant genotype AMS-MB-5-18 showed normal growth. But in both the treatments 0.7mM and 1.7mM of K2SiO3 (K2SiO3 + C.R. infected), TAMS-38, AMS-MB-5-18 and JS-335 showed tolerance to charcoal disease as well as healthy growth. To identify the amount of accumulation of silicon in the susceptible and resistant genotypes, SEM-EDX analysis was done. The result revealed higher deposition of the silicon in the trichomes of the susceptible genotype. Accumulation of Si in the genotypes and plant response against charcoal rot disease with different morphological observations was recorded. To assess the in-vitro effect of silicon at phenotypic level various morphological characters were recorded such as plant height (cm), number of branches, 50% flowering (D), days to maturity (D), number of pods per plant, grain yield per plant (g) and 100 seed weight (g). The analysis of variance showed significant difference between the genotypes of different concentrations of Si treated pots (0.7mM and 1.7mM of K2SiO3) with charcoal rot, control pot and Si control. The phenotypic screening of soybean genotype against charcoal rot disease revealed that K2SiO3 leads to better adaptability in soybean genotype under charcoal rot disease condition. The effect of Si was also studied at molecular level by using cDNA-RAPD profiling which revealed due to K2SiO3 treatments various genes were activated in response to charcoal rot disease. Each cluster represents different response towards charcoal rot in each individual genotype. Out of the total screened alleles 75 were monomorphic alleles with an average of 3.75 and 69 were polymorphic alleles with an average of 3.45. Results showed an average of 47.68 polymorphism percent. The IDVs (intensity derived values) were calculated to study expression of varying size of polymorphic amplicons. The highest IDV (241002) value was recorded for primer OPH-12 in 1.7 mM of K2SiO3 treated plant of susceptible genotype TAMS-38. The study revealed that both the treatments 0.7mM and 1.7mM of K2SiO3 showed healthier growth in all three soybean genotypes. 1.7mM of K2SiO3 treatment showed superior results at morphological as well as molecular level.