Loading...
Dr. Rajendra Prasad Central Agricultural University, Pusa
In the imperial Gazetteer of India 1878, Pusa was recorded as a government estate of about 1350 acres in Darbhanba. It was acquired by East India Company for running a stud farm to supply better breed of horses mainly for the army. Frequent incidence of glanders disease (swelling of glands), mostly affecting the valuable imported bloodstock made the civil veterinary department to shift the entire stock out of Pusa. A British tobacco concern Beg Sutherland & co. got the estate on lease but it also left in 1897 abandoning the government estate of Pusa.
Lord Mayo, The Viceroy and Governor General, had been repeatedly trying to get through his proposal for setting up a directorate general of Agriculture that would take care of the soil and its productivity, formulate newer techniques of cultivation, improve the quality of seeds and livestock and also arrange for imparting agricultural education. The government of India had invited a British expert. Dr. J. A. Voelcker who had submitted as report on the development of Indian agriculture. As a follow-up action, three experts in different fields were appointed for the first time during 1885 to 1895 namely, agricultural chemist (Dr. J. W. Leafer), cryptogamic botanist (Dr. R. A. Butler) and entomologist (Dr. H. Maxwell Lefroy) with headquarters at Dehradun (U.P.) in the forest Research Institute complex.
Surprisingly, until now Pusa, which was destined to become the centre of agricultural revolution in the country, was lying as before an abandoned government estate. In 1898. Lord Curzon took over as the viceroy. A widely traveled person and an administrator, he salvaged out the earlier proposal and got London’s approval for the appointment of the inspector General of Agriculture to which the first incumbent Mr. J. Mollison (Dy. Director of Agriculture, Bombay) joined in 1901 with headquarters at Nagpur
The then government of Bengal had mooted in 1902 a proposal to the centre for setting up a model cattle farm for improving the dilapidated condition of the livestock at Pusa estate where plenty of land, water and feed would be available, and with Mr. Mollison’s support this was accepted in principle. Around Pusa, there were many British planters and also an indigo research centre Dalsing Sarai (near Pusa). Mr. Mollison’s visits to this mini British kingdom and his strong recommendations. In favour of Pusa as the most ideal place for the Bengal government project obviously caught the attention for the viceroy.
Browse
129 results
Search Results
ThesisItem Open Access Morphological and bio chemical characterization of Winged bean Rhizobium and its impact as seed Inoculant either singly or Combinedly with PGPR towards Yield Attributing Characters and Yield of Winged Bean (Psophocarpus tetragonolobus)”(Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur, 2018) Kumari, Shweta; Yadav, R.C.A laboratory investigation for morphological and bio-chemical characterization and physiological properties of winged bean Rhizobium with a purified culture; and a field experiment consisting of four seed-inoculation treatments [No inoculation, Rhizobium, PGPR(Bacillus) and Rhizobium+PGPR(Bacillus)] and three nitrogen levels [0, 20 and 40 kg ha-1] in RBD (factorial) with three replications for evaluation of two winged bean germplasm IC-17002 and DLN-9, during crop season in the year 2014-15 and with better-performing IC-17002 in 2015-16 to find out impact of the Rhizobium as seed inoculant alone or in combinedly with PGPR on yield attributes and yield of winged bean were conducted at Tirhut College of Agriculture, Dholi Farm of RPCAU, Pusa (Bihar). The pure-culture of winged bean Rhizobium isolate was Gram-negative rod-shaped with a dimension of 0.59 μm x 1.81μm. The Rhizobium tested vis-à-vis (pea, gram, lentil and moong-rhizobia) showed a wide diversity in tolerance for its growth at different pH values, though neutral pH being the optimum. The winged bean-Rhizobium isolate produced 64.6 μg mL-1 of IAA at 72h of incubation with antibiotic-resistance and carbon-utilization test, the Rhizobium isolate was found to be resistant to vancomycin (30μg) and utilized a wider group of carbon sources than other rhizobia. The Fatty-Acid-Methyl-Ester (FAME) profiling confirmed that it belonged to Rhizobium-miscellany group. Seed inoculation and nitrogen levels greatly influenced nodule-parameters, nitrogenase activity and yield attributes, value of which were the highest with the combined inoculation of Rhizobium+PGPR (Bacillus), followed by seed inoculation of Rhizobium alone, and differed significantly among themselves at both 60 and 120 days after sowing (DAS). Nitrogen application @ 20 kg ha-1 recorded significantly higher nodule parameters over the control. The inoculation x nitrogen interaction effect was found to be significant with respect to nodule parameters. The combined inoculation of Rhizobium+PGPR (Bacillus) at 20 kg N ha-1 appeared significantly superior to other treatment combinations. S, Fe and Mo in the nodule recorded higher at 60 DAS than at 120 DAS. Rhizobium inoculation alone caused higher S and Mo content in nodule, whereas combined application of Rhizobium and PGPR recorded the highest Fe content at both growth stages. Nitrogen application @ 20 kg ha-1 increased Fe content significantly over no-nitrogen application at 120 DAS. The highest nitrogen content in pod was noted by combined inoculation of Rhizobium and PGPR treatment, followed by Rhizobium alone. Nitrogen @ 20 kg ha-1 was found to be superior to the higher level of N application. The combined inoculation of Rhizobium and PGPR at 20 kg N ha-1 proved better than the other treatments. The treatments recorded greater N and P content of winged bean plant at 60, 120 DAS and at harvest. In seed, Rhizobium and PGPR inoculation in combination recorded more N content than other treatments and was found to be at par with Rhizobium inoculation alone. Nitrogen application @ 20 kg ha-1 recorded higher N, P and K in seed of winged bean in first year only. Protein content in green edible pod, seed and tuber was significantly increased by combined inoculation of Rhizobium and PGPR over the control and was at par with the treatment of Rhizobium inoculation alone, whereas basal nitrogen @ 20 kg ha-1 favoured more protein content than higher level of nitrogen. After harvest of winged bean, the highest available soilnitrogen build-up was recorded with the treatment of Rhizobium inoculation alone. Seed-oil content (33.76 %) recorded the highest in the treatment with seed inoculation of PGPR at zero nitrogen application. The study proved the multiple-utilitarian potential of winged bean including its role in soil fertility improvement which was best with seedinoculation of Rhizobium-miscellany and PGPR either alone or in combination with basal dose of 20 kg N ha-1.ThesisItem Open Access Candidate gene markers based molecular profiling for grain zinc accumulation in rice(Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur, 2018) Kumari, Kumkum; Sharma, V.K.A study was conducted to determine the genetic variation and divergence in relation to grain zinc accumulation amongst rice varieties using candidate gene based panel of reported primers and to examine the genetic importance of zinc transporter candidate gene based panel of designed primers in discrimination for differential zinc accumulation amongst rice varieties. Twenty-eight locally adapted varieties and advanced breeding lines of rice were evaluated in randomized block design with three replications and the seeds collected after harvesting of the crop were utilized for determination of zinc content in unpolished grains. Eighteen entries selected from the two extremes of grain zinc distribution range constituted the final experimental material and utilized during molecular characterization. Genomic DNA was extracted from two to three weeks old seedlings of purposefully selected set of 18 varieties and then targeted amplification of the genomic DNA was achieved by using a panel of 14 candidate gene specific 14 reported primers and 14 designed primers. Exploitable extent of variability was observed with respect to grain zinc accumulation amongst the set of 28 rice varieties initially evaluated as experimental materials. Zinc content, which varied from 8.18 ppm to 21.53 ppm, was found to be considerably higher in unpolished grains of RAU 3036, Sanwal Basmati, Rajendra Nilam and Rajendra Bha gwati. Using a panel of 14 candidate genes specific 14 reported primer pairs, reproducible amplification was successfully achieved with 12 primer pairs amongst which only eight primer pairs generated polymorphic amplified products. Successful amplification with two candidate genes specific reported primers, namely, OsNAC and OsNRAMP6a was notachieved. Contrarily, each of the 14 designed primer pairs exhibited reproducible amplification, but polymorphic amplified products were generated with only eight primer pairs. Appearance of amplified products in the form of bands at different positions on the gel revealed differential migration due to differences in overall size of the products generated from targeted amplification of specific region of genome. Molecular level genetic polymorphism among the entries was recognized on the basis of variation in respect of position of bands. Ample genetic differentiation and divergence was revealed at the molecular level amongst the rice varieties subjected to molecular characterization using the candidate genes specific and polymorphic panels of reported as well as designed primer pairs. Results from reported primers and designed primers based analysis were in well agreement with each other. Furthermore, hierarchical classification pattern of rice varieties was almost completely corroborated by principal coordinate analysis based spatial distribution pattern of genetic profiles of rice varieties. Hierarchical cluster analysis as well as principal coordinate analysis based on a combination of polymorphic and informative eight reported and eight designed primer pairs provided better expression of differentiation and divergence amongst the rice varieties subjected to molecular characterization. Thus, the use of 14 candidate genes specific 16 polymorphic markers in the genetic analysis exhibited a remarkably higher level of genetic polymorphism, which allowed unique genotyping of eighteen entries included in the analysis. Hence, these markers can be effectively and efficiently utilized for grain zinc accumulation related discrimination of rice genotypes and selection of parental genotypes for genetic improvement in relation to grain zinc biofortification. Microsatellites were detected within the candidate genes and within the amplicons, thereby providing a basis to deduce that the variation present in candidate genes, as observed in terms of differences in the molecular size of the genomic regions spanned by the primer pairs, may be a role player in the differential grain zinc accumulation in rice varieties. Single marker analysis established the association of four markers, namely, OsNACK, OsZIP1-1, OsNRAMP7 and OsNRAMP7K with grain zinc accumulation. These four markers can be effectively used in marker-assisted selection program for grain zinc biofortification in rice. Inter-crossing diverse genotypes from different clusters can lead to successful pyramiding of desirable alleles through molecular breeding program. Parental genetic diversity will undoubtedly increase the probability of identifying desirable recombinants during screening for improvement in relation to grain zinc biofortification.ThesisItem Open Access Activities of Krishi Vigyan Kendra in Samastipur district of Bihar: an evaluative study(Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur, 2018) Kumar, Bipul; Shekhar, DibyanshuThe ICAR has established KVKs all over the country by making provision for multidisciplinary team of scientists. The institution is playing strategic role in technology back stopping, knowledge management and advisory to the stake holders like farmers, farm women, rural youths and extension personnel. The number of KVKs has risen to 645 and 106.more KVKs are to be established in the newly created districts and some larger districts. The KVK scheme is 100% financed by Govt. of India. The KVK, Samastipur was established in 2004 in 50 Acres of land at Birauli. which is 8 km away from the University headquarters and 15 km from the Samastipur district town. The KVK, Samastipur has under taken many interventions like training, OFT, FLD and other capacity building programmes to enhance the well being of farmers through enhancing production, productivity and profitability. The Krishi Vigyan Kendra, Birauli Samastipur has been bestowed with the award of “Best KVK (Zone-II)” by Hon‟ble Agriculture & Food Processing Industry Minister, Sri Sharad Pawar at the 8th National Conference on KVK organized at University of Agriculture Science, Bangaluru from 23rd-25th October, 2013.In view of the same, effort was made to undertake a study entitled. “Activities of Krishi Vigyan Kendra in Samastipur District of Bihar: An Evaluative Study” with the following specific objectives: To determine the coverage area through activities of Krishi Vigyan Kendra, Samastipur. To find out the socio-economic characteristic of the beneficiaries and non-beneficiaries. To explore the knowledge level and Extent of adoption of beneficiaries and non-beneficiaries farmers related with prime activities undertaken by the Krishi Vigyan Kendra. To assess the effect of different activities on the socio-economic development of beneficiaries. To examine the association between the selected independent variables and dependent variables. During 2016-17, all together 2842 farmers were trained by KVK, Birauli where as 759 farmers had benefited through different FLDs. More than Rs.8.0 lakh was earned by KVK through selling of planting materials, vermicmpost and publication where as 5000 farmers were benefited from other extension activities like Kisan Mela, Gosthi, diagnostic or field visit, advisories etc. more than 10 on farm trail were conducted for assessment and refinement. Majority of the respondents (88.89 per cent) belonged to middle to old age group having agriculture along with dairy and business as their major occupation. Further it was found that the annual income of majority of the respondents was in between 1 to 2 lakh per annum and most of the selected respondents had an educational attainment of intermediate and above. The majority (84.44 per cent) of the respondents belonged to small to marginal land holding category with a family size of more than 5 family members. In terms of farm mechanization, contact with extension agency, economic motivation the EDP beneficiaries‟ ranked top among others (i.e. FLD and non beneficiaries). In case of use of available source of information only 50% of total selected respondents used available source of information, out of them EDP beneficiaries again possess the major proportion. However, the EDP beneficiaries were the most frequently in contact with extension agency. It was also revealed during the study that EDP beneficiaries were found more aware related with knowledge of the activity of KVK undertaken in this study i.e., Mushroom production technology and vermicompost technology. While in case of knowledge level of FLD beneficiaries was having more knowledge about seed variety, package and practices of crop production than non-beneficiaries. Further in terms of adoption the vermicompost technology was most adopted by the beneficiaries and the impact of vermicompost training activities on the socio-economic development of beneficiaries possess high. During the study, it was found that age, land and source of income of the respondents were significantly associated with the knowledge level of the farmers. also, social participation improved technology.ThesisItem Open Access Cause effect relationship and genetic divergence study in cowpea [Vigna unguiculata (L.) Walp.](Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur (Bihar), 2018) Gupta, Ramesh Kumar; PramilaThe present investigation was carried out with twenty-seven genotypes of cowpea entitled "CAUSE-EFFECT RELATIONSHIP AND GENETIC DIVERGENCE STUDY IN COWPEA [Vigna unguiculata (L.) Walp.]” at Vegetable Research Farm of Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur, Bihar in Randomised Block Design with three replications during Zaid-2017. The quantitative traits under study were seed germination percentage, plant height, number of branches per plant, days to first flowering, first flowering node, number of nodes on main stem, peduncle length, number of pods per cluster, days to first picking, pod length, pod diameter, average pod weight, number of pods per plant, number of seeds per pod, test weight, pod borer infestation percentage and pod yield per plant. Statistical analysis were carried out to study Analysis of variance, Genotypic and Phenotypic coefficient of variation, Heritability, Genetic advance, Correlation coefficient analysis, Path analysis and Genetic diversity. Analysis of variance revealed highly significant differences among the genotypes for all the studied characters and also showed the presence of an ample amount of genetic variability among the genotypes. The phenotypic coefficient of variation was slightly higher than the corresponding genotypic coefficient of variation in magnitude. Indicating the role of genotype in the manifestation of these characters. High heritability coupled with high genetic advance as per cent of mean were recorded for all the traits except seed germination percentage and days to first flowering, indicating the preponderance of additive gene action suggesting that selection in an early generation will be effective. Plant height, number of branches per plant, number of nodes on main stem, number of pods per cluster, pod length, average pod weight and number of pods per plant had given positive and significant correlation comprising their positive and direct effect with pod yield per plant. Suggesting that during the course of selection these traits may be considered as prime traits for enhancing the yield in cowpea. The maximum inter-cluster distance was recorded between cluster II and V so, to have the heterotic recombinant for the seed germination percentage, days to first flowering, pod length, average pod weight, number of seeds per pod, number of pods per cluster, peduncle length, and pod diameter, for all these traits, genotypes present in these clusters may be used in crossing.ThesisItem Open Access Physiological response of lentil (Lens culinaris Medik.) genotypes to salinity stress and its mitigation through microbial inoculation(Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur (Bihar), 2018) Rani, Ediga Usha; KavitaLentil is an important legume crop which is highly sensitive to salinity and is adversely affected by salt stress in terms of growth and yield. Inoculation of plants with microbes such as Trichoderma viride, Bacillus, and Pseudomonas can enhance plant growth during salt stress, which is an eco-friendly approach to sustainable agriculture. The present investigation entitled “Physiological response of lentil (Lens culinaris Medik.) genotypes to salinity stress and its mitigation through microbial inoculation” was conducted with two objectives viz., to identify contrasting sets of lentil genotypes against salinity stress on the basis of physiological traits, and to study physiological response of microbes application in contrasting genotypes under salinity stress. For the identification of contrasting sets of lentil genotypes to salinity stress, experiment was performed in Petri dishes with seventeen genotypes. All genotypes were subjected to control and salinity stress (4.0 and 8.0 dSm-1) for 7 days and physiological parameters viz. germination percentage, seedling length, seedling dry weight, germination relative index, vigour index-І and vigour index-II were observed. Results showed a significant decrease in values of these parameters under salinity stress compared to control condition. Among genotypes, the least reduction in germination percentage, seedling length, seedling dry weight, germination relative index, vigour index-І and vigour index-II value were observed in genotypes RLG-234 and RLG-254 while the maximum percent reduction was observed in LL-931 and SJL 7-2. For the second objective, contrasting set of genotypes i.e., tolerant (RLG-234 and RLG-254) and susceptible genotypes (LL-931 and SJL 7-2) were sown in CRD with three replications in plastic pot filled with soil having EC 4.0 dSm-1. The contrasting genotypes were primed with different commercial formulation of microbes (Trichoderma viride, Pseudomonas fluorescence, Bacillus subtilis, Trichoderma viride + Pseudomonas fluorescence, Trichoderma viride + Bacillus subtilis, Pseudomonas fluorescence + Bacillus subtilis and Trichoderma viride + Pseudomonas fluorescence + Bacillus subtilis) and morpho-physiological and biochemical response under salinity stress was studied. Results showed that morphological (root length, shoot length, root dry weight, shoot dry weight) , physiological (relative water content, membrane stability index, total chlorophyll content) biochemical (proline, soluble protein, total free amino acids) and antioxidant enzymes (catalase, peroxidase, superoxide dismutase) were significantly increased in all the treatments over control (without microbial inoculation), whereas lipid peroxidation and protease activity was significantly decreased in all the treatments. Sodium content was decreased while potassium content was significantly increased in both root and shoot. Potassium- sodium ratio was higher in shoot than in root. On the basis of present study it was concluded that, salinity stress adversely affected morpho-physiological, biochemical attributes and nutritional content (Na and K) in lentil genotypes. These parameters were improved under salinity stress with the combined application of Pseudomonas fluorescence, Bacillus subtilis and Trichoderma viride showing synergistic response in ameliorating salinity stress.ThesisItem Open Access Physiology of mustard (Brassica juncea L. Czern and Coss) genotypes under salinity and high temperature stress at seedling stage(Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur (Bihar), 2018) Prasad, Satya Narayan; KavitaRapeseed-mustard is considered to be the second largest edible oilseed crop in the world after soybean. However, this is sensitive to salinity stress that adversely affects growth and yield. The crop also faces high temperature at seedling stage when sown late. The present investigation was carried out to screen mustard genotypes against salinity and high temperature stress conditions and to study physio-biochemical response in two contrasting genotypes under salinity and high temperature stress condition at seedling stage. The salt solution was prepared by using NaCl: CaCl2 in the ratio of 7:2 (w/v) and its electrical conductivity of different salinity levels (4.0 dSm-1 and 6.0 dSm-1) were maintained by direct reading conductivity meter. Twenty one genotypes viz., CS-52, CS-56, CS2002-61, CS2002-189, CS2002-195, CS2004-105, CS2004-106, CS2004-114, CS2004-191, CS2005-124, CS2005-125, CS2009-105, CS2009-145, CS2009-256, CS2009-261, CS2009-332, CS2009-347, CS2013-10, CS2013-19, CS2013-27 and CS1013-41 were subjected to primary screening at different stresses sown in seedling trays filled with soil. The treatments comprised of (a) Control (1.2 dSm-1) (b) 1.2 dSm-1 + High temperature (40℃) (c) Salinity of EC = 4.0 dSm-1 (d) Salinity of EC = 6.0 dSm-1 (e) Salinity (EC = 4.0 dSm-1) + high temperature (40℃) (f) Salinity (EC = 6.0 dSm-1) + high temperature (40℃) that were compared with control (1.2 dSm-1). The contrasting set of genotypes identified were used to study the physio-biochemical changes in 15-day old seedlings. On the basis of physiological parameters viz., survival percent, seedling length, dry weight of seedlings, vigour index-I and vigour index-II of contrasting set of genotypes were identified. Survival percent, seedling length, dry weight of seedlings, vigour index-I and vigour index-II declined in all the genotypes in different treatments over control, however, the decline was less in genotypes CS2009-347 and CS-52 whereas it was more in CS2009-256 and CS2009-145. The genotypes CS2009-347 and CS-52 were identified as relatively tolerant, whereas CS2009-256 and CS2009-145 were found to be susceptible genotypes. Stress application resulted in decline in membrane stability index, relative water content, chlorophyll content and soluble protein content; however, the decline was less in tolerant genotypes. Contrary to this, carotenoids content, proline content, catalase activity, peroxidase activity were found to increase, the increase being more pronounced in tolerant genotypes. Total free amino acids and protease activity also enhanced with the percent enhancement being more in susceptible genotypes. Results of this study will be helpful for finding salinity and high temperature tolerant genotypes for mustard improvement programme.ThesisItem Open Access Physiology of wheat (Triticum aestivum L.) under salt and high temperature stress condition(Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur (Bihar), 2018) Neelambari; Singh, Ajay KumarUnder natural conditions, plants are often subjected to a combination of different stresses such as salt stress and heat shock. Wheat crops are slightly salinity tolerant but as a cool season crop it is sensitive to heat stress at both vegetative and reproductive stages. Recent studies revealed that the response of plants to a combination of two different stresses is specific and cannot be deduced from the stresses applied individually because increased transpiration rate associated with high temperature stress worsen the harmful effects of salinity stress. Therefore, the present investigation entitled “Physiology of wheat (Triticum aestivum L.) under salt and high temperature stress condition” was conducted with three main objectives (1) Screening of wheat genotypes for combined salt and high temperature stress to identify the contrasting sets of wheat genotypes on the basis of physiological traits. (2) To study physiological and biochemical mechanisms of tolerance of wheat genotypes subjected to independent, and combined salt and high temperature stress conditions. (3) To quantify the changes in yield and yield attributes of wheat genotypes grown under independent, and combined salt and high temperature stress conditions. In the present study, two independent experiments were conducted. The first experiment was done in laboratory condition where 46 wheat genotypes were screened for individual and combined salinity and high temperature stress condition at seedling stage. Wheat seeds were sown in petri plate in three replications for germination. A saline solution of composition NaCl:CaCl2: Na2SO4 (7:2:1) with salinity levels at 4.0 and 8.0 dS m-1 were used as irrigation solution for giving salt stress treatments. High temperature treatment, was given by keeping the petri dishes in incubator at temperature 37 ± 2°C, and combined salinity and high temperature stress was given by transferring salinity stressed plant to incubator at high temperature (37 ± 2°C). The physiological parameters were measured in 10-day-old seedling. Physiological parameters (germination percentage, shoot length, seedling length, shoot fresh weight, root fresh weight, seedling fresh weight, root dry weight, shoot dry weight, seedling dry weight, vigour index I, vigour Index II and SPAD value) were measured and found to be reduced, except root length which was found to be increased in some genotypes and decreased in others under salinity, high temperature and combined stress. However the reduction was more pronounced under combined stress. On the basis of physiological data, the contrasting set of wheat genotypes (tolerant genotypes, i.e., KRL-1-4, KRL-19 and HD-2733, and susceptible genotypes, i.e., HT-8, HI-1563 and HD-2987) were identified for further study. The genotypes identified from screening experiment were used for a pot culture experiment to study the physiological and biochemical mechanism of stress tolerance and yield/yield associated parameters. Three replications of pot were arranged in factorial completely randomized design. Four treatments were given, i.e., control (wheat genotypes were sown timely in soil with ECe 1.3 dS m-1), salinity stress treatment (wheat genotypes were sown timely in natural saline soil with ECe 7.4 dS m-1), high temperature stress treatment (wheat genotypes were sown late in soil with ECe 1.3 dS m-1) and combined salinity and high temperature stress treatment (wheat genotypes were sown late in natural saline soil with ECe 7.4 dS m-1). The physiological and biochemical parameters were measured in flag leaf at anthesis period and yield parameters were measured at physiological maturity. The result revealed a decrease in physiological parameters in all wheat genotypes, while in tolerant group the decrease was less in all parameters, i.e., relative water content (25.0-29.6%), membrane stability index (23.9-27.2%), total chlorophyll (41.1-42.4%) and carotenoids content (14.9-17.2%), chlorophyll stability index (30.8-36.2%) and SPAD value (26.1-28.7%) as compared to susceptible group of genotypes in which percentage change in relative water content ranged from 37.7 to 41.2%, membrane stability index (37.9-44.5%), total chlorophyll (52.6-60.2%) and carotenoids content (15.1-33.9%), chlorophyll stability index (44.6-49.9%) and SPAD value (33.5-40.9%) under combined salinity and high temperature stress treatments. However, lipid peroxidation was increased in all the genotypes with minimum percentage increase in tolerant group which ranged from 58.6 to 60.3% and maximum percentage increase in susceptible group ranged from 71.4-88.3% under combined salinity and high temperature stress treatments. Greater antioxidant enzyme activities were induced in tolerant group, i.e., peroxidase (61.8-69.8%) and superoxide dismutase activity (75.4-86.9%). Similarly, proline content (61.1-68.1%), total phenol content (59.9-71.9%), total soluble sugar (42.5-48.9%), and free amino acids (43.3-50.9%) were higher in tolerant types, in comparison to susceptible group, i.e.,, peroxidase (40.6-49.2%), superoxide dismutase activity (50.7-56.3), total soluble protein (12.2-24.4%), proline content (37.6-47.4%), total phenol content (43.8-49.0%), and free amino acids (21.1-32.2%) under combined stress condition. However, total soluble protein content was found to be decreased less in tolerant group (19.6-21.6%) then in susceptible group (36.6-28.4%) under combined stress condition. Irrespective of the genotypes, combined stress reduced the yield and yield components in all genotypes. However, the reduction was minimum in tolerant genotypes, i.e., plant height (25.8-30.6%), number of grain per ear (28.6-34.1%), test weight (29.2-31.9%), harvest index (36.7-39.0%) and yield per plant (37.6-43.6%) as compared to susceptible genotypes in which reduction in plant height ranged from 36.9 to 49.6%, number of grain per ear 44.4 to 49.4%, test weight 38.7 to 43.4%, harvest index 47.3 to 50.3% and yield per plant 53.5 to 58.3% under combined stress treatments. However, floret sterility index was increased in all the genotypes with minimum percentage increase in tolerant group which ranged from 67.0-74.1% and maximum percentage increase in susceptible group ranged from 96.3 to 101.2% under combined salinity and high temperature stress treatments. From the present investigation it was evident that tolerant genotypes, i.e., KRL-19, KRL-1-4 and HD-2733 had the potential to cope up with the adverse effect of given stress treatments and they performed well under stress condition with least reduction in physiological, yield and yield associated parameters and greater increase in biochemical parameters. Among all parameters, antioxidants enzyme activity (SOD), phenol and proline content were highly correlated with yield per plant and showed maximum induction under combined salinity and high temperature stresses. Hence, the higher percentage increase in these parameters may be the reason for the tolerance of genotypes, HD-2733, KRL-19 and KRL-1-4. Possibly, some of these indices might prove useful for improving wheat genotypes to withstand combined salinity and high temperature.ThesisItem Open Access Physiological response of Mung bean [Vigna radiata L. (Wilczek)] genotypes to application of salicylic acid and trichoderma under salinity stress(Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur (Bihar), 2018) Kumar, Suresh; KavitaThesisItem Open Access Stability study in spring planted early maturing sugarcane clones for higher yield(Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur (Bihar), 2018) Kumar, Praveen; Pandey, S.S.Fifteen early maturing sugarcane genotypes were planted in spring season at two different locations viz. Pusa and Madhopur for two consecutive years (2016 and 2017) to assess their stability across four environments viz. E1 – Pusa‟2016, E2 – Madhopur‟2016, E3 – Pusa‟2017 and E4 – Madhopur‟2017, in Randomized Block Design with three replications for yield and attributing traits. The genotypes showed high amount of variability present in individual (E1, E2, E3 and E4) and pooled over environments for most of the characters. Genotypes viz. CoP 16437, CoP 16436, CoP 11437 and CoP 16438 showed superiority for most of the traits including sugar and cane yield. PCV was found higher than GCV revealed environmental influence, sugar yield exhibited high heritability coupled with high GAM revealing preponderance of additive effects of gene while cane yield at harvest showed high heritability coupled with moderate GAM. Significant G × E interaction including environment (linear), linear component of G × E interaction and pooled deviation (non-linear) were significant indicating considerable genetic variability for most of the studied traits. Role of the environmental variation was observed for most of the attributes except for the number of shoots at 120 days, plant height (PH) at 240 days, single cane weight (SCW), number of milliable cane (NMC) and cane yield (CY) at harvest revealing no effect of environment or similar behaviour and response of studied genotypes. As per estimated stability parameters, six genotypes were found stable viz. CoP 16437, CoP 11438 and BO 153 stable for CY and SY in rich environment. CoP 16436 stable in poor environment for CY while in rich environment stable for SY. CoP 16438 stable in poor and rich environment for SY and CY respectively whereas CoP 11437 was stable for CY in rich environment. In AMMI analysis, G × E interaction was found highly significant for most of the traits, IPCA I and IPCA II were also found highly significant for most of the traits. IPCA I and IPCA II cummulatively contributed more than 80 percent of the total G × E interaction for all traits. All four environments E1, E2, E3 and E4 were identified as favourable for cane yield and sugar yield at harvest and fall into two groups i.e. one group (E2 and E4) and another group (E1 and E3) were preferred for most of the traits and had similar effects on genotypes. Biplot analysis revealed that genotypes CoP 16437, CoP 16436, CoP 11437, CoP 16438, BO 153 and CoP 11438 were found the most desirable and favourable across different environments. As per both the stability model the genotypes viz. CoP 16437, CoP 16436, CoP 11438, CoP 16438 and CoP 11437 were identified as stable one for yield and yield attributing traits in their respective environments.