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Dr. Rajendra Prasad Central Agricultural University, Pusa

In the imperial Gazetteer of India 1878, Pusa was recorded as a government estate of about 1350 acres in Darbhanba. It was acquired by East India Company for running a stud farm to supply better breed of horses mainly for the army. Frequent incidence of glanders disease (swelling of glands), mostly affecting the valuable imported bloodstock made the civil veterinary department to shift the entire stock out of Pusa. A British tobacco concern Beg Sutherland & co. got the estate on lease but it also left in 1897 abandoning the government estate of Pusa. Lord Mayo, The Viceroy and Governor General, had been repeatedly trying to get through his proposal for setting up a directorate general of Agriculture that would take care of the soil and its productivity, formulate newer techniques of cultivation, improve the quality of seeds and livestock and also arrange for imparting agricultural education. The government of India had invited a British expert. Dr. J. A. Voelcker who had submitted as report on the development of Indian agriculture. As a follow-up action, three experts in different fields were appointed for the first time during 1885 to 1895 namely, agricultural chemist (Dr. J. W. Leafer), cryptogamic botanist (Dr. R. A. Butler) and entomologist (Dr. H. Maxwell Lefroy) with headquarters at Dehradun (U.P.) in the forest Research Institute complex. Surprisingly, until now Pusa, which was destined to become the centre of agricultural revolution in the country, was lying as before an abandoned government estate. In 1898. Lord Curzon took over as the viceroy. A widely traveled person and an administrator, he salvaged out the earlier proposal and got London’s approval for the appointment of the inspector General of Agriculture to which the first incumbent Mr. J. Mollison (Dy. Director of Agriculture, Bombay) joined in 1901 with headquarters at Nagpur The then government of Bengal had mooted in 1902 a proposal to the centre for setting up a model cattle farm for improving the dilapidated condition of the livestock at Pusa estate where plenty of land, water and feed would be available, and with Mr. Mollison’s support this was accepted in principle. Around Pusa, there were many British planters and also an indigo research centre Dalsing Sarai (near Pusa). Mr. Mollison’s visits to this mini British kingdom and his strong recommendations. In favour of Pusa as the most ideal place for the Bengal government project obviously caught the attention for the viceroy.

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20211
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Subject: Agricultural Biotechnology × Author: NARAYAN, SHINDE ANAND × End date: 2023 × Start date: 2021 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    MORPHO-MOLECULAR CHARACTERIZATION AND GENETIC DIVERSITY ANALYSIS FOR DROUGHT TOLERANCE IN CHICKPEA
    (DRPCAU, Pusa, 2021) NARAYAN, SHINDE ANAND; KUMAR, MITHILESH
    The present study was undertaken with twenty-eight germplasm accessions of Cicer arientinum L. having different geographical origin, obtained from germplasm collections at T.C.A., Dholi, The experiment was carried out at T.C.A., Dholi Research Farm of Dr. R.P.C.A.U., Pusa, Samastipur, Bihar, in a completely Randomized Block Design with three replications during kharif, 2020-21 for molecular characterization and diversity analysis in chickpea. Data were collected for twelve characters viz., Plant height, Days to 50% flowering, No. of Pods per plant, Main root length, Main root girth, Root volume, Root dry weight, No. of nodule per plant, Nodule dry weight, 100 seed weight and yield per plant. Statistical analysis carried out were, Analysis of variance, genotypic and phenotypic coefficient of variation, heritability, genetic advance and molecular characterization. Analysis of variance showed highly significant differences among the genotypes for all the twelve characters under study. Maximum range was found for the No. of nodule per plant followed by Days to 50% flowering. The phenotypic coefficient variance (PCV) was greater than genotypic coefficient variance (GCV), heritability and genetic advance as percent of mean were observed highest for Root volume, Root dry weight, No. of Nodule per plant and Nodule dry weight indicating that these traits can be used as selection indices for yield improvement in drought condition. Seventeen SSR primer pairs for molecular characterization of 18 chickpea genotypes were used which has generated 116 total alleles out of which 45 are unique alleles in the form of amplified products. The PIC values revealing allele diversity and frequency among the chickpea genotypes varied from 0.593 in ICCM0178 to 0.859 in ICCM0190a. Among all the primers, seven primers showed highest polymorphism according to the PIC that are ICCM0010b, H1A19, H1G11, H2A02, H2E13, H2I10, ICCM0190a with the value 0.803, 0.846, 0.815, 0.815, 0.821, 0.834, 0.859 respectively. The polymorphism per cent of the primer ranges from 20% to 57.14% with lowest in H1009 and highest in H1A06 respectively. According to the cluster analysis and the principle coordinate analysis, the eighteen chickpea genotypes were differentiated into four groups. Cluster I had the 7 genotypes, cluster II had 2 genotypes, cluster III had 4 genotypes and last cluster IV had 5 genotypes.