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Dr. Rajendra Prasad Central Agricultural University, Pusa

In the imperial Gazetteer of India 1878, Pusa was recorded as a government estate of about 1350 acres in Darbhanba. It was acquired by East India Company for running a stud farm to supply better breed of horses mainly for the army. Frequent incidence of glanders disease (swelling of glands), mostly affecting the valuable imported bloodstock made the civil veterinary department to shift the entire stock out of Pusa. A British tobacco concern Beg Sutherland & co. got the estate on lease but it also left in 1897 abandoning the government estate of Pusa. Lord Mayo, The Viceroy and Governor General, had been repeatedly trying to get through his proposal for setting up a directorate general of Agriculture that would take care of the soil and its productivity, formulate newer techniques of cultivation, improve the quality of seeds and livestock and also arrange for imparting agricultural education. The government of India had invited a British expert. Dr. J. A. Voelcker who had submitted as report on the development of Indian agriculture. As a follow-up action, three experts in different fields were appointed for the first time during 1885 to 1895 namely, agricultural chemist (Dr. J. W. Leafer), cryptogamic botanist (Dr. R. A. Butler) and entomologist (Dr. H. Maxwell Lefroy) with headquarters at Dehradun (U.P.) in the forest Research Institute complex. Surprisingly, until now Pusa, which was destined to become the centre of agricultural revolution in the country, was lying as before an abandoned government estate. In 1898. Lord Curzon took over as the viceroy. A widely traveled person and an administrator, he salvaged out the earlier proposal and got London’s approval for the appointment of the inspector General of Agriculture to which the first incumbent Mr. J. Mollison (Dy. Director of Agriculture, Bombay) joined in 1901 with headquarters at Nagpur The then government of Bengal had mooted in 1902 a proposal to the centre for setting up a model cattle farm for improving the dilapidated condition of the livestock at Pusa estate where plenty of land, water and feed would be available, and with Mr. Mollison’s support this was accepted in principle. Around Pusa, there were many British planters and also an indigo research centre Dalsing Sarai (near Pusa). Mr. Mollison’s visits to this mini British kingdom and his strong recommendations. In favour of Pusa as the most ideal place for the Bengal government project obviously caught the attention for the viceroy.

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20201
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Subject: Agricultural Biotechnology × Author: Lalaso, Abhang Sachin × End date: 2020 × Start date: 2020 × Type: Thesis ×
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    ThesisItemOpen Access
    Amplification profiling of grain zinc accumulation related candidate genes in rice using TRAP markers
    (DRPCAU, Pusa, 2020) Lalaso, Abhang Sachin; Sharma, V.K.
    The present study was undertaken to examine the molecular level genetic polymorphism in relation to grain zinc concentration among 18 genotypes of rice using 12 candidate gene specific TRAP markers. The purpose of the present investigation was to evaluate the extent of genetic variability and diversity in respect of grain zinc concentration among these rice genotypes. Differential amplification profile was ascertained amongst the genotypes subjected to molecular characterization by employing 12 TRAP markers. Altogether 141 polymorphic bands representing easily recognizable different types of amplified products were categorized into 29 unique products and 112 shared products. Polymorphism information content of 12 TRAP markers diverged from 0.437 to 0.776 around an average measure of 0.611 across the markers. The combination of fixed forward primers OsNRAMP7K, OsZIP3K, OsZIP4a and OsZIP5K with an arbitrary reverse primer ME02 generated the lowest number of polymorphic products. Furthermore, the forward primer OsZIP7K in combination with the same arbitrary reverse primer yielded the highest number of polymorphic products. The forward primers OsZIP1-1, OsZIP7K, OsZIP4a, OsZIP5K, OsZIP4K, OsYSL14K and OsZIP5-3 along with ME02 yielded remarkably larger allelic size difference in descending order of magnitude. Numerical variation in polymorphism information content of the markers reflected their allelic diversity and distribution frequency among the genotypes, which diverged from 0.437 for the combination of primer OsZIP5-3+ME02 to 0.776 for the combination of primer OsZIP7-2+ME02 with an average of 0.611 across all the primer pair combinations. The fixed forward primers OsZIP7-2, OsZIP1-1 and OsZIP4K in combination with ME02 seemed to be extremely polymorphic and informative, whereas the remaining nine primer pair combinations were found less polymorphic. Similarity coefficients measuring the genetic similarities on the basis of the total count of shared polymorphic amplified products among pair-wise combinations of the genotypes revealed sizeable molecular level genetic variation among the genotypes. Experimental results clearly reflected the existence of plentiful diverseness at the molecular level among the 18 genotypes under evaluation in the present study. Hierarchical classification pattern based on target region amplification polymorphism related genetic similarity among rice genotypes was almost in complete agreement with principal coordinate analysis based two-dimensional dispersion pattern of molecular genetic profiles of rice genotypes. Using both the modules, the genotypes were conveniently grouped into four considerably discriminated multi-genotypic groups highly consistent with their grain zinc concentration. Candidate gene markers based analysis appeared remarkably effective in differentiation of rice genotypes in relation to their grain zinc concentration. Hence, these markers can be efficaciously and expeditiously employed for grain zinc concentration associated distinction of rice genotypes and extraction of rice genotypes for their utilization as parental lines during genetic enhancement in respect of grain zinc biofortification. Significance of zinc transporters based target region amplification polymorphism markers for differentiation and divergence analysis was established in relation to grain zinc concentration in rice genotypes.