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Dr. Rajendra Prasad Central Agricultural University, Pusa

In the imperial Gazetteer of India 1878, Pusa was recorded as a government estate of about 1350 acres in Darbhanba. It was acquired by East India Company for running a stud farm to supply better breed of horses mainly for the army. Frequent incidence of glanders disease (swelling of glands), mostly affecting the valuable imported bloodstock made the civil veterinary department to shift the entire stock out of Pusa. A British tobacco concern Beg Sutherland & co. got the estate on lease but it also left in 1897 abandoning the government estate of Pusa. Lord Mayo, The Viceroy and Governor General, had been repeatedly trying to get through his proposal for setting up a directorate general of Agriculture that would take care of the soil and its productivity, formulate newer techniques of cultivation, improve the quality of seeds and livestock and also arrange for imparting agricultural education. The government of India had invited a British expert. Dr. J. A. Voelcker who had submitted as report on the development of Indian agriculture. As a follow-up action, three experts in different fields were appointed for the first time during 1885 to 1895 namely, agricultural chemist (Dr. J. W. Leafer), cryptogamic botanist (Dr. R. A. Butler) and entomologist (Dr. H. Maxwell Lefroy) with headquarters at Dehradun (U.P.) in the forest Research Institute complex. Surprisingly, until now Pusa, which was destined to become the centre of agricultural revolution in the country, was lying as before an abandoned government estate. In 1898. Lord Curzon took over as the viceroy. A widely traveled person and an administrator, he salvaged out the earlier proposal and got London’s approval for the appointment of the inspector General of Agriculture to which the first incumbent Mr. J. Mollison (Dy. Director of Agriculture, Bombay) joined in 1901 with headquarters at Nagpur The then government of Bengal had mooted in 1902 a proposal to the centre for setting up a model cattle farm for improving the dilapidated condition of the livestock at Pusa estate where plenty of land, water and feed would be available, and with Mr. Mollison’s support this was accepted in principle. Around Pusa, there were many British planters and also an indigo research centre Dalsing Sarai (near Pusa). Mr. Mollison’s visits to this mini British kingdom and his strong recommendations. In favour of Pusa as the most ideal place for the Bengal government project obviously caught the attention for the viceroy.

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Subject: Agricultural Biotechnology × Author: Kumari, Priti × End date: 2017 × Start date: 2017 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Characterization of wheat (Triticum aestivum L.) genotypes for disease reaction against spot blotch pathogen
    (Dr. Rajendra Prasad Central Agricultural University, Pusa (Samastipur), 2017) Kumari, Priti; Kumar, Rajeev
    Wheat (Triticum aestivum L.) is the largest consumed cereals and most important element in the diet of the poor. Successful production of the wheat in the warmer regions of South Asia is constrained by several biotic and abiotic stresses. Spot blotch of wheat caused by Bipolaris sorokiniana is one of the most significant biotic stresses which limits the production and productivity of the crop in the region. Under present investigation eighty-eight genotypes of wheat were evaluated for the purpose of twelve morpho-metric traits and reaction against spot blotch disease in randomized block design (RBD) during rabi, 2015 Molecular characterization using 17 SSR markers (associated with tolerance against spot blotch disease) was also undertaken to as certain the genetic diversity among the studied genotypes Observations on 1000-grain weight, grain filling duration (GFD), no. of tiller per plant, canopy temperature, yield per plot, heading, days to greenness, disease severity and area under disease progress curve (AUDPC), lesion size, leaf tip necrosis, biomass per square meter were recorded. Significant differences were observed among the genotypes for all the traits under consideration. Genotype × environment interactions were found non-significant. Molecular characterization work revealed a total of 232 alleles in 19 genotypes using 17 SSR markers. The number of alleles per locus varied from four in Xgwm371 to thirty in Xgwm 445 with an average of 11.04 alleles per locus. Total 165 unique alleles were observed at 21 SSR loci, with an average of 7.85 unique alleles per locus. The number of unique alleles per locus ranged from one in Xgwm 371 to twenty six in Xgwm 445. Out of 17 SSR primers 10 showed null alleles. The maximum 4 genotypes (Ariana, Dharwad Dry, Ciano T 79 and HD 2967) exhibited null alleles for the primer pair Xgwm 120. Stutter bands were also detected in the present investigation. Such bands were observed in the case primer pairs, Xgwm-611, Xgwm-159 Xgwm 132, Xgwm 445, Xgwm 371, Xgwm146, Xgwm 425. The PIC values varied from 0.435 in the case of Xgwm120 to 0.959 in the case of Xgwm 146 with an average of 0.876 across the primer, indicating that the markers were highly informative (PIC>0.5). The use of SSR markers in the present analysis exhibited a remarkably higher level of genetic polymorphism and allowed unique genotyping of the genotypes included in the analysis. The nineteen genotypes classified into five different clusters or groups (I to V) at 22 phenon level based on cluster analysis using the UPGMA method based on Dice coefficients.