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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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Subject: Sericulture × Author: Jyoti, Biradar × End date: 2019 × Start date: 2010 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Molecular characterisation of root specific mapping population of mulberry by SSR markers and identification of QTLs Governing drought tolerance traits
    (University of Agricultural Sciences, Bengaluru, 2013-09-20) Jyoti, Biradar; T. K, Narayanaswamy
    Mulberry leaf is the only food source for the silkworm Bombyx mori L, hence drives the silk industry in India. There is an intimate connection between Mulberry and the silkworm. India is the second largest silk producer by contributing to 18% of the world silk requirement. But, the industry faces major constraints between demand and supply due to the lack of improved varieties. The advancement of sericulture industry depends upon the quantitative and qualitative improvement of mulberry varieties. It is strongly opined that application of genomic tools would be mandatory to achieve this. DNA based markers have proved to be the choice of the system. Among them SSRs found to be more promising because of their co-dominance, multiallelic capabilities. Though SSR markers are potential, their availability in mulberry is very much limited. The present study focused to develop more number of SSR markers in mulberry and construct a linkage map for molecular breeding applications. Pre cloning enrichment strategy was adopted for the marker development. A total of 222 genomic and 136 genic markers were developed out of 126 Mb of the mulberry genome. Out of 358 markers, 188 were found locus specific and were characterized in diverse mulberry species and genotypes. The transferability of these markers was also assessed in three other related species belonging to the family Moraceae viz., Ficus, Fig and Jackfruit. All the markers showed single locus amplification in morus species and genotypes and 79.25% were transferable to cross species. The phylogenetic relationship of the 14 mulberry species and genotypes belonging to the family Moraceae were revealed according to their evolutionary pedigree. The locus specific markers were further used for mapping using 150 F1 population generated by the cross between high rooting genotype Dudia white and low rooting genotype UP105. The linkage map was constructed based on the segregation pattern of the markers using Mapmaker V 3.0 software. A total of 82 out of 150 markers segregating in 1:1 ratio could aligned in 14 linkage groups covering 3330.9cM of the mulberry genome. All the linked markers were randomly aligned over the linkage groups accounting an average of 40.6cM between two markers. Of the 14 linkage gropus LG1 and LG4 were dense with maximum number of markers. The linkage map was further used to identify markers associated with quantitative trait loci (QTL) for rooting traits in mulberry. The segregating population was screened for four quantitative traits contributing to root and water use efficiency and their association with the linked markers. The mapping population showed a normal distribution pattern for all the traits. Significant association between marker and traits were established for root length, root weight, total leaf area and total dry matter by single marker regression (SMR) on the basis of analysis of variance (ANOVA) at p<0.05 significance level. The present study was an important effort towards the improvement of genomic wealth of mulberry.