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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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20231
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Subject: Plant Biochemistry × Start date: 2023 ×
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Now showing 1 - 3 of 3
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    ThesisItemEmbargo
    STUDY ON ANTIFUNGAL ACTIVITY OF SOIL MICROBIAL METABOLITES AGAINST Fusarium solani AND Sclerotium rolfsii
    (2023-01-17) ARUNA, P. M.; MOHAN CHAVAN
    Research to develop biological control agents against the plant pathogens to replace the synthetic agrochemicals gained the considerable attention. The present study was conducted to assess the potential antagonistic bacteria against fungal pathogens from three different regions of Karnataka viz. Yana caves, Dandeli and GKVK campus Bengaluru. Sixty isolates were screened against Fusarium solani and Sclerotium rolfsii, among them one showed antagonism against Sclerotium rolfsii and three were showing antagonism against Fusarium solani. Further, morphological, biochemical and molecular characterization revealed that Bacillus subtilis strain OTG009 showed inhibition (55.55 %) against Sclerotium rolfsii, similarly Bacillus subtilis strain JC43, Bacillus subtilis strain P and Pseudomonas aeruginosa DSM 50071 showed inhibition (45.55 %, 43.33 %, 63.33 % respectively) against Fusarium solani. The isolates found that were able to produce siderophores(chelating agents).However, only Pseudomonas aeruginosa able to produce HCN which helps in defense against plant pathogens. This established the basis for further investigation. Spectrophotometer analysis showed that the extract showed maximum absorption from 200 nm to 400 nm indicated the present of active compounds. The qualitative analysis of compounds present in the ethyl acetate extract using GCMS revealed the presence of antimicrobial, antifungal, antibacterial compounds. Pseudomonas aeruginosa was effectively inhibiting the pathogen growth in vitro. These beneficial soil microbes can be used for the control of plant fungal diseases caused by Fusarium solani and Sclerotium rolfsii.
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    ThesisItemOpen Access
    Biochemical stuides on the development of aril browning inpomegranate
    (University of Agricultural Sciences GKVK, Bangalore, 40729) Hemlata Singh; Shivashankar, S
    Aril browning (AB) in pomegranate is a physiological disorder free of external symptoms. Browning of aril starts with a dark dot on the aril and spreads further to the entire aril. The incidence is at first observed at 50% fruit maturity near the calyx end just under the skin. Present studies showed that AB incidence was higher in panicles with increasing number of fruits and in fruits located on the lateral shoots as compared to those on main shoots. Fruits exposed to sun showed lesser incidence. AB incidence also increased with fruit maturity. Biochemical studies revealed that sugars, TSS, starch and pH were higher in AB affected aril as compared to healthy arils whereas anthocyanin, polyphenols, titrable acidity, protein and ascorbic acid were less in AB affected aril. Enzyme activities like amylase, total dehydrogenase activity in seed were reduced in seed of AB affected aril compared to healthy whereas enzyme activity like polyphenol oxidase was more in seed of AB affected aril as compared to seed of healthy aril. Healthy arils showed higher moisture content and the seed higher percentage and faster rate of germination as compared to seed of AB affected aril, revealing that seed of AB affected aril had lost moisture leading to reduction in seed viability. Field experiments with growth regulators showed that GA3 treatment reduced incidence of AB and PBZ treatment increased the incidence of browning as compared to control. These findings indicated that the development of AB in pomegranate is a result of combination of many factors like interfruit competition, biochemical and physiological changes in aril during fruit growth.
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    ThesisItemOpen Access
    PURIFICATION AND CHARACTERIZATION OF AN ANTIVIRAL PROTEIN FROM THE SILKWORM Bombyx mori L.
    (University of Agricultural Sciences GKVK, Bangalore, 41113) ASHISH, MARATHE; ANITHA, PETER
    Sericulture is an important agrobased industry. India stands second next to China among the silk producing countries of the world. Silkworm is susceptible to fungal, bacterial, viral and protozoan diseases. The Bombyx mori nucleopolyhedrosis virus (BmNPV) is the most harmful virus in the sericulture industry. The Antiviral Red Fluorescent Protein from the silkworm was purified from the digestive juice and the excreta of the silkworm. The red fluorescent protein (RFP) was isolated and purified by ammonium sulfate saturation, organic solvent precipitation and gel filtration chromatography on sephadex G-100 column. The fractions that fluoresce red in U.V were used for further analysis. The protein from the digestive juice and excreta were subjected to Native and Denaturating PAGE. The electrophoregram of the purified protein from the digestive in native PAGE has shown two bands and the SDS – PAGE pattern for the RFP from the gut fluid revealed the presence of four different bands. The electrophoregram of the purified protein form the feacal matter in native PAGE showed a single band which coincided with the first band in the protein from the gut juice. The SDS-PAGE pattern revealed two bands of which one was found to be around 28 kDa and the other band was smaller than 14.4 kDa. Dot Blot was carried out to detect the specificity between the antigen and the antibody. ELISA was also done to fix the titre of the antigen and antibody. The titre obtained was 1:25 of antigen and 1:2000 of primary and secondary antibody dilutions.