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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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Subject: Biotechnology × Author: LAKSHMANA REDDY, DC × Start date: 2006 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Induction of Programmed cell death in tobacco Bright Yellow-2 cell lines and Saccharomyces cerevisiae strainYPH-500 against various apoptotic stimuli
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU, 2008-02) LAKSHMANA REDDY, DC; Mathew, M K
    Programmed cell death (PCD) is a fundamental cellular process conserved in metazoans, plants and yeast. Our results suggested that hydrogen peroxide, heat and salinity induces PCD in tobacco bright yellow-2 cell lines and yeast strain YPH 500; these results are comparable to earlier reports. In tobacco BY-2 cell lines, hydrogen peroxide induces programmed cell death at 10mM concentration and typical features of PCD like membrane blebbing, DNA fragmentation, ROS rise and MMP loss were observed. Vacuole morphology varied during PCD process against H2O2, heat and salinity stresses. l. PCD observed in yeast against H2O2, heat and salinity stresses with typical hallmarks like DNA fragmentation, ROS rise and MMP loss. The concentration of H2O2 required in yeast is lower than the tobacco cells. In case of salinity, yeast requires higher (1.5M) as against 200mM in tobacco cells; there is no difference in induction of PCD with respect to heat stress in both systems. The death percentages were reduced by pre incubating yeast cells with calcium and ROS chelator and scavengers respectively. In yeast cells, elevation of ROS levels was observed against NaCl and heat stress and the elevation of cytosolic calcium levels was observed against H2O2 and NaCl stress. The rise of calcium levels was reduced by pre incubation with EGTA. The rise of calcium levels is less in H2O2 stress compare to saline stress. The reduction in percentage of cell death was observed in yeast cells against H2O2, Heat and NaCl by preincubation with scavengers. TEMPOL and Glutathione reduce cell death against H2O2 and Heat, where as EGTA reduces cell death against H2O2, Heat and NaC In our study, results conclude, both tobacco BY-2 cell and yeast cells exhibit substantially similar PCD characters against H2O2, Heat and NaCl stresses