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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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Subject: Biotechnology × Author: KASSAHUN BANTTE BISETEGN × Start date: 2006 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    MAPPING OF SIMPLE SEQUENCE REPEATS (SSRS) AND MARKER-ASSISTED INTROGRESSION OF QUANTITATIVE TRAIT LOCI (QTLS) FOR STAY-GREEN IN SORGHUM [SORGHUM BICOLOR (L). MOENCH]
    (University of Agricultural Sciences GKVK, Banglore, 2007-08-30) KASSAHUN BANTTE BISETEGN; Dr. M.S. KURUVINASHETTI
    With the objective of increasing the marker density in the linkage map of the stay-green mapping population, ninety four individuals from N13 x E36-1 sorghum recombinant inbred population were genotyped with 78 EST-SSR makers and 55 markers (70%) gave reliable and scorable amplification. The genotypic data from these markers was integrated with the genotypic data of 164 previously mapped RFLP, AFLP, RAPD and SSR markers to produce the genetic maps. The genetic map constructed has a total length of 2838 cM. The number of EST-SSR markers mapped per linkage group ranged from 2 (linkage group H) to 9 (linkage group F). Some of the markers such as Xisep0841, Xisep0733, and Xisep0938 mapped in the stay-green QTL regions making them good candidate markers to be used in fine mapping of the QTLs and marker-assisted selection of the stay-green trait. In a marker-assisted backcrossing programme, four stable stay-green QTLs {StgB, Stgl, Stg3 and Stg4) were selected to be transferred from the slay-green donor parent (B35) to the senescent recurrent parent (R16). After two additional marker-assisted backcrossing and two selling generations, four backcross families with single {Stgl, Stg3, Sg4 and StgB) and five backcross families with double putative QTL^ {Stg1+Stg3, Stg1+Stg4, Stg3+Stg4, Stg3-hStgB and Stg4+StgB) have been selected. Previously generated, early generation stay-green introgression lines (BC2F2/BC1F3) were evaluated for their agronomic and stay-green related characters under well watered and water-stress conditions both in field (in the post rainy season) and greenhouse condition for two seasons. Among the introgression lines, RSG 04001/RSG 05001 was found to be the most promising line both in terms of its grain yield and stay-green related characters. Besides high % green leaf area, the stay-green genotypes had high chlorophyll content and leaf nitrogen concentration compared with the senescent lines showing the relationships among these traits. The correlations of relative grain yield to relative % GLA in the stress environments was highly significant (R^= 0.47 in 2004-05 and R^= 0.77 in 2005-06) indicating the contribution of stay green to grain yield.