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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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Subject: Biotechnology × Author: K. KRISHNAMURTHY × Start date: 2006 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    POST-TRANSCRIPTIONAL GENE SILENCING (PTGS) OF TOMATO LEAF CURL VIRUS (ToLCV) IN TOMATO
    (University of Agricultural Sciences GKVK, Banglore, 2007-08-30) K. KRISHNAMURTHY; P. U. KRISHNARAJ
    Tomato, an economically important crop in many countries is plagued by many viral diseases including leaf curl caused by Tomato leaf curl virus (ToLCV) belonging to the genus begomovirus. Begomoviruses are small circular, single stranded DNA plant viruses. Yield losses usually vary from 28-92% making tomato cultivation unprofitable. Genetically engineering resistance is a viable alternative is to genetically engineer tomato for protection against ToLCV. PTGS/RNAi is a novel gene regulatory mechanism that limits the transcript level by either suppressing transcription or by activating a sequence-specific RNA degradation process. We have cloned and characterized ToLCV-coat protein (TCP), replicase (TRP) and suppressor of PTGS (TRS) genes from a Dharwad local isolate. Constructs have been developed using all the three genes for the available gene silencing strategies viz., sense (s), antisense (as), ihp (sas) and HUTR (heterologous 3'-untranslated region). Coat protein (TCP) gene has been cloned and expressed in a prokaryotic system. As a comparative study plant expression vectors carrying TCP, TRP and TRS gene following different strategies were used for transgenic development through Agrobacterium. Analysis of putative To-transgenics showed positive for PCR, GUS. Dot blot and Southern blot analysis. Semi-quantitative PGR analysis of plants from TRP constructs showed drastic reduction in the virus inoculum compared to non-transgenic plants. The Ti-generataion transgenic plants obtained from TRP constructs were positive for PCR and Dot blot analysis. Among different strategies tested for resistance to ToLCV in transgenics, those with sas/ihp construct showed significant resistance against ToLCV followed by HUTR, antisense (as) and sense (s). Similarly, among the three different genes tested, silencing was more in TRS constructs followed by TRP and TCP.