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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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Subject: Animal Biotechnology × Start date: 1985 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    STUDIES ON SOMATIC EMBRYOGENESIS IN PAPAYA CV. SURYA WITH REFERENCE TO ENDOPHYTIC BACTERIAL ASSOCIATION
    (University of Agricultural Sciences GKVK, Bangalore, 40625) PRABIR KUMAR, DAS; PIOUS, THOMAS
    Papaya (Carica papaya L.) is an important fruit crop world over. Micro propagation of shoot buds approach has not been very successful for commercial propagation purpose. Feasibility of somatic embryogenesis from immature zygotic embryos is also suggested in papaya. A protocol has now been optimized for somatic embryogenesis from immature zygotic embryos of papaya cv. Surya. Immature zygotic embryos were established on half-strength MS medium with supplements (MS vitamins, 6 per cent sucrose, 10 mg l-1 2, 4-D, 400 mg l-1 glutamine and 0.8 per cent agar (pH 5.7) giving high frequency and repetitive embryogenesis after 3-4 subculture passages. Continuous proliferation of somatic embryos was achieved on maturation and regeneration medium containing half-strength MS constituents, 3 per cent sucrose, 400 mg l-1 glutamine, 0.2 mg l-1 BAP and 0.1 mg l-1 NAA and 0.8 per cent agar. Medium containing a combination of 0.5 mg l-1 IAA and 0.5 mg l-1 IBA was ideal for root development. Endophytes are generally known to originate from the rhizosphere or phyllosphere and seeds are generally considered to be free from endophytes. Tissue and medium indexing on visibly clean embryogenic cultures initiated from immature seeds showed the association of cultivable bacteria in a small section (1.4 per cent) of cultures after three subculture passages. One bacterium was isolated from such cultures, which is identified as Brachybacterium rhamnosum based on 16S rRNA gene sequence homology analysis. PCR-screening of visibly clean and index negative cultures using universal bacterial 16S rRNA gene primers indicated the presence of non-cultivable bacteria in index-negative cultures. This was confirmed by microscopy of tissue homogenate revealing viable bacteria in considerable number.