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Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar
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ThesisItem Restricted Studies on the effects of ochratoxin - A ,ascorbic acid and zinc sulphate on the pathology and pathogenesis of hydropericardium syndrome in broiler chicken(LUVAS, 2004) D. Madhuri; Verma, P.C.A study has been conducted to find out the effects of ochratoxin–a, ascorbic acid and zinc sulphate on the pathology and pathogenesis of hydropericardium syndrome in broiler chicken. A total of 320 , day old broiler chicks were divided into four groups of 80 chicks each. The chicks in group AC, ZC and OC were given ascorbic acid@330 ppm , zinc sulphate @700 ppm and ochratoxin A @1.5 ppm in the feed ,respectively from day 1 till the end of the experiment. Group XC served as control. At the age of 21, chicks in all the groups were further divided into 2 subgroups XC & XH, AC & AH, ZC & ZH and OC&OH respectively. The birds in groups XH, AH, ZH and OH were inoculated with 0.5 ml of diluted HPSL inoculum subcutaneously at day 21 of age . Except for decreased growth rate in OC group no other clinical signs were observed in control groups. Following infection with HPSL inoculum chicks in infected groups exhibited clinical signs which were more severe in group OH followed by XH, ZH and AH in descending order. A high mortality (60.60%) in group OH, moderate (30.30 %) in group XH and low mortality (12.12%&18.18%)was observed in groups AH and ZH. Significant decrease in body weights in group OC was observed as compared to other control groups. Within infected groups decrease in body weight was more in groups OH &XH as compared to groups AH & ZH. Serum enzymatic analysis showed significant decrease in TSP, albumin and globulins in OC group birds as compared to other control groups. A significant decrease in TSP, albumin and increase in AST, ALT &LDH activities was observed in all infected groups as compared to their respective control groups. Among infected groups incorporation of feed with ascorbic acid or zinc sulphate caused improvement in enzymatic activities whereas ochratoxin A caused increase in severity of disease. Immunological studies indicated decrease in humoral and cell mediated immune response in infected groups as compared to their respective control groups. Grossly, the lesions were similar in all infected groups though they persisted for long duration in OA treated birds .However ,less number of birds showed gross lesions in ascorbic acid and zinc sulphate supplemented groups and recovery from infection was also early in these groups. Significant increase in relative liver and spleen weight and decrease in bursa weight was observed in infected groups as compared to control groups. Histopatholgical alterations comprising basophilic I/N inclusion in the hepatocytes, depletion of lymphocytes in bursa, spleen, thymus ,degenerative changes in kidney tubules and necrosis of myocardium were more severe and persisted for longer duration in OH group birds whereas these were less severe in AH and ZH groups birds. In conclusion ochratoxin A enhanced the severity whereas ascorbic acid and zinc sulphate supplementation reduced the mortality, development of lesions in HPS infected birds.ThesisItem Restricted Etio-pathological studies on gastrointestinal tract disorders of sheep(LUVAS, 2005) Sharma, Vikas; Pruthi, A.K.The present investigation was carried out to study the clinico-haematological aspects and pathology of gastrointestinal tract disorders in sheep in the state of Haryana. . A total of 100 faecal and blood samples were analysed from 100 diarrhoeic sheep for clinico-haematological studies. Examination of faecal samples revealed the presence of Haemonchus contortus and Trichostrongylus spp.. Bacteriological examination of faecal samples revealed E. coli as most predominant bacterial organism followed by Proteus spp., Klebsiella spp. and Salmonella typhimurium 4, 5: i:1,2. E. coli strains belonged to 20 different serotypes. O88 was the most prominent serotype followed by O172, O44, O2, O153, O91, O80, O25, O132, O158, O1, O3, O5, O42, O73, O101, O109, O128, O147 and O156 Haematological analysis showed a significant decrease in values of haemoglobin, packed cell volume, total erythrocyte count, total leucocyte count, mean corpuscular volume, mean corpuscular haemoglobin concentration and mean corpuscular haemoglobin. Differential leucocyte count and absolute counts of leucocytes revealed a significant increase in values of neutrophils and eosinophils whereas a significant decrease in values of lymphocytes was observed. Serum biochemical examination revealed a significant decrease in values of total serum protein, serum albumin, albumin: globulin ratio, cholesterol and glucose whereas a significant increase in serum globulin was observed. An increase in the activities of alanine aminotransferase and alanine aminotransferase was observed in diarrhoeic sheep, beside this, a significant decrease in sodium and an increase in potassium levels was also observed. In pathological studies, 112 sheep carcasses revealed changes in gastrointestinal tract during postmortem examination. The gross lesions in oesophagus, intestines and abomasum were petechial haemorrhages and congestion of mucosa. Liver evinced petechial haemorrhages and congestion, yellow discolouration, abscess formation and necrotic foci. Gross lesions in other visceral organs along with gastrointestinal tract were congestion and consolidation of lungs, haemorrhages and congestion of spleen, heart and kidney and swollen mesenteric lymph node. Histopathological, lesions in intestines and abomasum were desquamation of epithelium, infiltration of neutrophils, lymphocytes, macrophages and plasma cells. Liver exhibited degenerative changes viz. cloudy swelling, fatty changes and hydropic degeneration along with infiltration of neutrophils, lymphocytes, macrophages and plasma cells in liver parenchyma. Coagulative necrosis and chronic hepatitis with connective tissue proliferation were also noticed in liver. Mesenteric lymph node was reactive and exhibited depletion of lymphocytes in lymphoid follicles. Spleen also revealed depletion of lymphocytes in lymphoid follicles in white pulp and reticular cell hyperplasia. Lungs exhibited congestion and infiltration of neutrophils and lymphocytes in alveoli along with presence of serous exudate. Heart showed congestion and sarcocysts in myocardium. Kidney revealed congestion and hyaline and epithelial cast in tubular region. Bacteriological studies of different organs of sheep carcasses revealed E. coli as most predominant bacterial organism followed by Proteus spp., Klebsiella spp., Salmonella typhimurium 4,5:i:1,2 Staphylococcus spp., and Pseudomonas aeruginosa. E. coli strains belonged to 14 different serotypes. O88 and O153 were most predominant serotypes followed by O158, O22, O25, O44, O172, O42, O156, O91, O132, O32, O64 and O70. Antibiogram pattern of different bacterial organisms isolated from faecal samples of diarrhoeic sheep and different organs of sheep carcass revealed that bacterial organisms were most sensitive to amikacin and gentamicin, whereas most resistant to tetracycline and nitrofurantoin. Mortality studies of sheep during period of six year (1999 to 2004) based on postmortem records revealed pneumonia and enteritis as major cause of death. Mortality was higher in lambs than adults. In female mortality was higher than that of male. In season wise mortality studies, mortality was maximum in summer season followed by rainy season and winter season.ThesisItem Restricted Etiopathomorphological studies of buffaloes/buffalo calves mortality(LUVAS, 2006) Seema; Jakhar, K.K.The present study was envisaged to understand the pathological, bacteriological and parasitological aspects of hepatic tract disorders in buffaloes. Data pertaining to thirty buffaloes /buffalo calves brought for post mortem to the of Department of Veterinary Pathology showing gross lesions in liver revealed that mortality was maximum in buffalo calves of age group up to 1 month. Regarding sex of buffalo calves, mortality was much higher in females. Systemwise disease noticed were hepatitis, gastritis, enteritis, gastroenteritis and pneumonia in buffalo/buffalo calves. Gross pathological changes in various organs noticed were congestion and haemorrhages in liver, necrotic foci on liver surface, hard indurated liver and hepatomegaly. In case of lungs there was congestion, haemorrhages, fibrinous pneumonia along with adhesions. In kidneys there was congestion, haemorrhages and adhesions. Congestion, thickened capsule and splenomegaly along with adhesions were seen in spleen. Heart revealed congestion, haemorrhages, hydropericardium and adhesions. Lymph nodes were hard. Congestion along with haemorrhages was evidenced in intestines. Histopathologically there was congestion, haemorrhages in liver. Hydropic degeneration, fatty changes, necrotic changes in parenchyma, infiltration of leucocytes, proliferation of fibroblast, bile duct hyperplasia and atrophy of hepatocytes were also seen. Lung revealed congestion, edema in pleura and parenchyma, thickened pleura due to fibroblast proliferation, pseudolobulation and leucocytic infiltration in interstitial tissue. Kidney showed congestion, haemorrhages, coagulative necrosis, fibroblast proliferation, leucocytic infiltration in interstitial tissue and atrophy of glomeruli. In spleen, there was congestion, thickening of capsule, depletion of lymphocytes in white pulp and reticulo endothelial cell hyperplasia. Heart showed edema congestion, haemorrhages, fragmentation and necrosis of muscle fibres and leucocytic infiltration. Lymph node revealed congestion in cortex and medulla, depletion of lymphocytes in germinal center of lymphoid follicle, necrosis and cystic structure formation and fibroblast proliferation. In intestine there were desquamation of mucosa. Congestion, goblet cell hyperplasia, infiltration of leucocytes and ulceration. Cultural isolation of suspected etiological agents from carcasses of buffalo/ buffalo calves revealed that E.coli was most prevalent bacteria followed by Pseudomonas spp., Klebsiella spp., Citrobacter divergens .E.coli serotype O9 was most prevalent serotype followed by O121, O20, O21, O132, O101 and many other less frequent were O12, O6, O8, O12, O73. A total of 21 strains were isolated, 5 strains were rough and 5 were untypable. Strains isolated from liver were O8, O9, O12, O19, O20, O101, O128, O132 and Rough. Strains isolated from liver along with other organs were O9, O20, O132, Rough.O8 strain was isolated only from liver. Parasitic examination of intestinal contents revealed infestation of Strongyle spp. along with Eimeria spp.ThesisItem Restricted Studies on effects of vitamin e supplementation on pathology and pathogenesis of experimentally induced hydropericardium syndrome in broiler chickens(LUVAS, 2007) Nehra, Vikas; Gupta, R.P.The present investigation was conducted to study the effect of Vitamin E supplementation on pathology and pathogenesis of Hydropericardium syndrome (HPS) in broiler chickens. One hundred and twenty (120) broiler chicks at the age of 14 days were divided randomly into two groups (group A and B) of sixty birds each. Group A birds were given feed mixed with Vitamin E @ 150mg/kg feed through out the experiment, while the birds of group B were kept on feed without Vitamin E supplementation. At the age of 21 days, blood samples were collected from five birds from each group which were then sacrificed and thereafter each group was further divided into two subgroups (group A into A1 and A2 and group B into B1 and B2) of 35 and 20 birds, respectively. Each bird of group A1 and B1 was injected with 0.5 ml of the 10-4 diluted HPS virus infected liver inoculum (ID50), subcutaneously. Haematological and biochemical alterations, gross and histopathological changes were studied on 2nd, 4th, 6th and 9th day post infection. Cell mediated immune response (CMI) was assessed by delayed type hypersensitivity (DTH) skin contact test. The clinical signs exhibited in HPS infected birds were anorexia, dullness, depression, ruffled feathers and chest resting posture with closed eyes. However, these clinical signs were of milder nature in Vitamin E supplemented HPS infected group. The mortality due to HPS infection was relatively less in Vitamin E supplemented HPS infected group as compared to HPS infected without Vitamin E supplemented group. There was no significant effect on body weight gain in both the infected groups. The haematological studies revealed a significant reduction in Hb and PCV in HPS infected without Vitamin E supplemented chickens from days 4 to 6 PI. A non significant reduction in TEC was observed in both the infected groups. There was a significant decrease in MCV values of HPS infected without Vitamin E supplemented group but difference in MCHC values between HPS infected without Vitamin E supplemented group as compared to control was non significant indicating microcytic normochromic anemia. Serum biochemical studies revealed a significant reduction in serum total protein concentration in both the HPS infected groups. This reduction was lower in magnitude in Vitamin E supplemented HPS infected group as compared to HPS infected without Vitamin E supplemented group. Significant reduction in serum albumin concentration was observed in HPS infected without Vitamin E supplemented group. There was significant increase in the serum ALT activity in both the infected groups that was of lower magnitude in Vitamin E supplemented HPS infected group. There was a non significant increased in serum AST activity in HPS infected without Vitamin E supplemented chickens. A significant increase in serum CPK activity was observed in HPS infected without Vitamin E supplemented chickens. The difference in the values of serum albumin concentration, AST and CPK between Vitamin E supplemented HPS infected group and control was non significant throughout the experiment. The gross lesions observed in HPS infected chickens were hydropericardium, congested and swollen liver with necrotic and petaechial haemorrhagic spots on the surface, oedematous lungs and congested thymus. The severity of these lesions was markedly reduced due to Vitamin E supplementation. Histopathological lesions observed in HPS infected chickens were congestion and hepatitis along with intranuclear basophilic inclusion bodies in liver; myocarditis, fragmentation of myocardial muscles; oedema in pericardium and haemorrhages in heart; perivascular oedema, congestion and interstitial pneumonia in lungs, lymphocytic depletion along with congestion and haemorrhages in bursa of Fabricius, spleen and thymus; focal interstitial nephritis in kidneys; congestion, pancreatitis along with necrosis and presence of intranuclear inclusion bodies in ascinar cells in pancreas. There was a marked reduction in the severity of these microscopic lesions in liver, heart, lungs and kidneys due to Vitamin E supplementation. No histopathological lesions were noticed in lymphoid organs and pancreas in Vitamin E supplemented HPS infected group. Percent protective effect due to Vitamin E supplementation in HPS infected group was indicating that Vitamin E supplementation has reduced the severity of pathological lesions. DTH response against DNCB antigen was significantly more in Vitamin E supplemented group as compared to control and HPS infected without Vitamin E supplemented group chickens indicating enhanced CMI response due to Vitamin E supplementation. It is concluded that supplementation of Vitamin E reduced the severity of HPS infection and enhanced the CMI response, suggesting the protective role of Vitamin E in limiting the pathology and pathogenesis of HPS infection in broiler chickens.ThesisItem Restricted Etio-pathological studies on calf mortality with special reference to hepatic disorders in bovine calves(LUVAS, 2008) Lehreena; Gupta, R.P.The detailed postmortem examination of 52 carcasses of buffalo-calves and cow-calves was conducted from May, 2007 to February, 2008 received in the Department of Veterinary Pathology. Mortality was maximum in the age group of 1-3 months in buffalo-calves and of less than 1 month in cow-calves. The bacterial organisms isolated were E.coli (11 cases), Klebsiella pneumonae (5), Proteus mirabilis (4), Pseudomonas aeruginosa (3), Staphylococcus aureus (3), Corynebacterium pyogenes (3), Streptococcus pyogenes (2), and Citrobacter divergens (1). Various serotypes of E.coli isolates were O60, O20, O114, O164, O4 and O12. In six isolates, serotypes were untyped. In-vitro drug sensitivity and resistance against different organisms revealed that E.coli isolates were most sensitive to ceftriaxone + sulbactum and most resistant against oxytetracycline and streptomycin. 100% isolates of Proteus and Pseudomonas were sensitive to ceftazidime and gentamycin, respectively, Klebsiella isolates to cefotaxime and furoxone, Corynebacterium isolates to ceftriaxone + sulbactum and kanamycin whereas Staphylococcus and Streptococcus isolates were 100% sensitive to the most of the antibiotics. Out of 19 E.coli isolates, 18 E.coli isolates possessed plasmids of molecular weights ranged from 4 to 20 Kb. The serotypes which were resistant to one or more than four antibiotics were found to possess plasmid of high molecular weight i.e. 20 Kb. The results of PCR revealed that 20 samples were found to be positive for E.coli as they gave an amplified product of 232bp. The examination of faecal samples collected from intestinal contents revealed two cases of Strongyles sp and three were of Eimeria sp. Gross and histopathological studies revealed that E.coli infected calves showed congestion and haemorrhages in various organs, hepatitis, serofibrinous pneumonia, catarrhal enteritis or haemorrhagic enteritis, focal interstitial nephritis, myocarditis and fibrinous pericarditis. Gram-negative red rod shaped bacilli were also demonstrated in hepatic and pneumonic lesions by Taylor’s stain. The characteristic lesions in Proteus infection were hepatitis, suppurative or fibrinous pneumonia, catarrhal or haemorrhagic enteritis, focal interstitial nephritis in kidneys, myocarditis and depletion of lymphocytes along with haemosiderosis. The characteristic lesions observed in Klebsiella pneumonae were suppurative bronchopneumonia, haemorrhagic enteritis or suppurative enteritis, mild fibrosis along with bile duct hyperplasia and fatty changes in liver and depletion of lymphocytes in spleen. Staphylococcus aureus infection was characterized by hepatitis with mild fibrosis in portal triad area in liver, acute interstitial pneumonia, haemorrhagic and catarrhal enteritis and acute interstitial nephritis. Streptococcus infection in calves was characterized by perivascular oedema in lungs, enteritis either haemorrhagic or catarrhal, pericarditis and suppurative myocarditis in heart and haemorrhages in liver and kidneys. Corynebacterium pyogenes infection was characterized by the presence of hepatitis and fibrous tissue proliferation in liver, bronchopneumonia, haemorrhagic enteritis and myocarditis. One case of cow-calf revealed tuberculosis granuloma in which red coloured coccobacilli were demonstrated by Ziehl Neelsen stain. Various mixed infections were also encountered like E.coli, Citrobacter divergens and Pseudomonas aeruginosa infection in buffalo-calf which was characterized by pyogranuloma in liver, serous pneumonia, catarrhal enteritis, fatty changes in myocardium, congestion and haemorrhages in kidneys. Another mixed infection was of E.coli and Pseudomonas aeruginosa, which was characterized by hepatitis, catarrhal enteritis, chronic myocarditis and interstitial nephritis. Mixed infection of Klebsiella pneumonae and Pseudomonas aeruginosa was characterized by thrombosis and hepatitis in liver, fibrinous pneumonia, mild myocarditis, catarrhal enteritis and congestion in kidneys. The results were also described on the basis of pathological conditions. Maximum cases observed were of haemorrhagic/catarrhal enteritis (37 cases) followed by hepatitis (35 cases), pneumonia (31 cases), myocarditis and pericarditis (14 cases) and interstitial nephritis (8 cases); nephrosis (5 cases)ThesisItem Restricted Studies on the efficacy of inactivated vaccines against hydropericardium syndrome in broiler chickens(LUVAS, 2005) Malik, Shakti; Jakhar, K.K.The present study was undertaken to study the efficacy of inactivated vaccines against hydropericardium syndrome in broiler chickens. At the age of five days, 251 broiler chicks were randomly divided into three groups viz. A, B and C comprising 81, 81 and 89 chicks, respectively and then vaccinated. Chicks of groups A and B were given oil emulsion and formalin killed vaccine, respectively @ 0.5ml s/c per bird whereas no vaccine was given in group C. At the age of 21 days, birds in groups A was divided into sub groups AVX (20) and AVH (53); B into BVX (20) and BVH (53) and C into CX (28) and CH (53). The chicks in groups AVH, BVH and CH were challenged with 0.5ml of diluted (10-3) of 30 per cent HPS inoculum (ID50) subcutaneously. The birds of all the six subgroups were observed for clinical signs, mortality and growth response. For biochemical studies, blood was collected from four randomly selected birds from each group at 0, 1, 3, 5, 7 and 11 days post challenge (DPC). For sequential pathological studies, four randomly selected birds from all groups were sacrificed at above mentioned intervals. Immune response was assessed by skin type hypersensitivity reaction and agar gel precipitation test. Clinical signs exhibited in group CH were anorexia, dullness, depression, ruffled feathers and abnormal posture with birds resting their chest on the cage floor. Eighteen birds (40%) died in group CH, whereas only four (8.8 %) and three birds (6.6%) died in groups AVH and BVH, respectively. There was significant decrease in body weight in group CH in comparison to control group. The severity of clinical signs, mortality and effect on body weight was maximum in group CH, followed by group AVH and least in group BVH. Total serum proteins, albumin and globulins decreased significantly in HPS challenged birds (group CH). In vaccinated challenged groups, the levels of these parameters were significantly more than those in group CH. The activities of serum alkaline phosphatase, lactate dehydrogenase, alanine aminotransferase and aspartate aminotransferase increased significantly in unvaccinated HPS challenged birds (group CH). The enzyme activities in vaccinated challenged groups (AVH and BVH) were significantly less when compared to unvaccinated challenged birds (group CH). Salient gross changes were hydropericardium, congested and swollen liver with necrotic and hemorrhagic spots, congested and oedematous lungs, atrophied bursa of Fabricius, congested thymus and kidneys and enlarged spleen. Histopathological lesions in unvaccinated challenged birds mainly included degenerative, necrotic and vascular changes in various organs and depletion of lymphocytes in different organs along with mononuclear cell infiltration. Average gross and microscopic lesion scores in unvaccinated challenged, formalin killed challenged and oil emulsion vaccinated challenged groups were 22.25, 6.00, 7.50 per cent and 40.50, 3.00 and 7.00 per cent, respectively. The pathological changes in group BVH were, in general, similar to group AVH but of lesser intensity and evidenced at later stage of the experiment. Delayed type hypersensitivity was maximum in group BVH followed by groups AVH, CH and was minimum in group CX. Agar gel precipitation test evidenced clear precipitation lines in group BVH, faint lines in group AVH and no precipitation line in control group (CX) throughout the experiment. On the basis of these studies, it is reasonable to conclude that the efficacy of formalin killed vaccine appeared to be comparatively more than the oil adjuvant vaccine.ThesisItem Restricted Etio-pathological studies on poultry mortality with special reference to gastrointestinal tract disorders(LUVAS, 2009) Hooda, Ashish Kumar; Mishra, S.K.The present investigation was undertaken to study etio-pathology with special reference to gastrointestinal tract disorders on poultry mortality. Data pertaining to the last five years (January 2004-December 2008) mortality in poultry revealed that mortality was maximum in adult age group (29.11%) and affections of digestive system accounted for highest mortality (30.88%). The detailed postmortem examination of all carcasses of poultry was conducted from July, 2008 to March, 2009 received in the Department of Veterinary Pathology and Department of Veterinary Epidemiology and Preventive Medicine, CCS HAU, Hisar. Representative tissue pieces from 65 chickens and 5 pigeons revealing changes in gastrointestinal tract were collected. Gross and histopathological studies revealed that E.coli infected birds showed fibrinous perihepatitis and pericarditis, catarrhal exudate in the lumen of intestines, serofibrinous exudate in lungs, appearance of cystic spaces with reticuloendothelial cell hyperplasia in bursa of Fabricius. In the cases of ascites syndrome too, E. coli was isolated indicating the role of same organism in pathogenesis of the disease. The characteristic lesions noticed in poultry having Salmonella spp. infection were presence of multiple necrotic foci in liver and spleen, red hepatisation in lungs and depletion of lymphoid tissue in bursa of Fabricius. The characteristic lesions in Proteus infection were consolidated and hemorrhagic lungs with serofibrinous exudates and hemorrhages on the epicardium. Citrobacter freundii infection in birds was characterized by the congestion and hemorrhages in lungs, heart and intestines. The characteristic lesions observed in Klebsiella pneumonae were severe congestion in lungs, fatty liver and atrophied bursa of Fabricius. Clostridium perfringens infection was characterized by septicaemic carcasses with presence of necrotic foci on liver and hemorrhagic enteritis. Corynebacterium pyogenes was also isolated along with E. coli and Salmonella Pullorum. Lesions present in this infection were presence of necrotic foci on liver and hemorrhagic lungs which may be induced by other bacteria. Lesions observed in birds affected with coccidiosis were hemorrhagic enteritis, thickened intestinal mucosa and ballooning of intestines. Histopathologically, lesions were mainly present in the intestines with different stages of coccidial life cycle in mucosal epithelial cells. Sarcocystosis was also observed in one case of pigeon with presence of Miescher’s tubes containing Rainey’s corpuscles in the myocardium. Isolation of bacteria was attempted from 50 carcasses of poultry. Different types of bacterial organisms isolated were Escherichia coli (39 cases), Salmonella Pullorum 9,12 (5), Salmonella Gallinarum 9,12 (9), Salmonella Enteritidis 9,12:g,m (5), Proteus vulgaris (9), Klebsiella pneumoniae (3), Citrobacter freundii (3), Clostridium perfringens (2) and Corynebacterium pyogenes (1). Mixed infection was found in 22 cases. Fourteen different serotypes [O2 (11), O4 (7), O7 (1), O20 (5), O22 (4), O24 (3), O56 (6), O71 (4), O78 (7), O83 (2), O101 (6), O120 (5), O139 (1) and O152 (3)] of E. coli were isolated. Five strains were untypable and 9 were rough. Antibiogram pattern revealed all bacterial agents were most resistant to bacitracin, lincomycin and oxytetracycilne. Majority of isolates were sensitive to ceftrioxone, cefuroxime, amoxyclav, chloramphenicol, gentamycin and amoxycillin. All 79 E.coli isolates possessed plasmids of molecular weights ranged from 3 Kb to 20 Kb. The results of PCR revealed that all 79 samples were found to be positive for E.coli as they gave an amplified product of 232bp. The examination of faecal samples collected from intestinal contents revealed that coccidiosis was the only parasitic disease condition diagnosed in 29 birds through faecal examination of the intestinal contents of the poultry.